Phenotypes associated with this allele

• Allele Symbol: Ptpn11^tm5Bgn
• Allele Name: targeted mutation 5, Benjamin Neel
• Allele ID: MGI:3512153
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptpn11^tm5Bgn/Ptpn11^tm5Bgn	involves: 129S4/SvJae * C57BL/6J	MGI:3810967

Genotype
MGI:3810967

hm1

• Allelic Composition: Ptpn11^tm5Bgn/Ptpn11^tm5Bgn
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality at implantation, incomplete penetrance ( J:106624 )

• abundance of homozygotes at E3.5 is normal but few are found at E6.5 or E7.5

• the few implanted embryos are poorly organized, necrotic, and show little tissue differentiation

embryo

decreased trophectoderm cell proliferation ( J:106624 )

• trophoblast stem cells show reduced proliferation and colony formation in culture

abnormal endoderm development ( J:106624 )

• endodermal cell lines specified but either fail to proliferate or fail to survive

abnormal blastocyst morphology ( J:106624 )

• marked increase in apoptosis after 48 hours in culture

• hatch in culture at around 3 days with dramatically reduced cell numbers

• only a thin layer of epithelioid cells remain by day 5

abnormal trophectoderm morphology ( J:106624 )

• trophectoderm cell lines are specified but either fail to proliferate or fail to survive

reproductive system

impaired embryo implantation ( J:106624 )

♀ • few implanted embryos

♀ • embryos that do implant are poorly organized, necrotic, and lack tissue differentiation

cellular

decreased trophectoderm cell proliferation ( J:106624 )

• trophoblast stem cells show reduced proliferation and colony formation in culture