Phenotypes associated with this allele

• Allele Symbol: Mtor^tm1.1Koz
• Allele Name: targeted mutation 1.1, Sara C Kozma
• Allele ID: MGI:3512186
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mtor^tm1.1Koz/Mtor^tm1.1Koz	either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * C57BL/6)	MGI:3512563
ht2	Mtor^tm1.1Koz/Mtor^+	either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * C57BL/6)	MGI:3512566

Genotype
MGI:3512563

hm1

• Allelic Composition: Mtor^tm1.1Koz/Mtor^tm1.1Koz
• Genetic Background: either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:94133 )

• no homozygous embryos are found at E11.5 or E12.5

embryo

abnormal developmental patterning ( J:94133 )

• at E5.5 the embryos appear disorganized with no clear extraembryonic or embryonic boundaries

embryonic growth arrest ( J:94133 )

• homozygous embryos arrest around E5.5 at the early egg cylinder stage and by E7.5 are disorganized and improperly oriented in the yolk sac cavity

embryonic growth retardation ( J:94133 )

• at E6.5 and E7.5 homozygous embryos are severely growth retarded

abnormal embryonic tissue morphology ( J:94133 )

inner cell mass degeneration ( J:94133 )

• the cells of the inner cell mass fail to proliferate in culture and no ES cell lines could be generated from homozygous mutants

abnormal extraembryonic tissue morphology ( J:94133 )

small ectoplacental cone ( J:94133 )

• the ectoplacental cone fails to elongate after E5.5

abnormal trophoblast layer morphology ( J:94133 )

• trophoblast outgrowth is impaired in culture

growth/size/body

embryonic growth retardation ( J:94133 )

• at E6.5 and E7.5 homozygous embryos are severely growth retarded

Genotype
MGI:3512566

ht2

• Allelic Composition: Mtor^tm1.1Koz/Mtor⁺
• Genetic Background: either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * C57BL/6)

normal phenotype

no abnormal phenotype detected ( J:94133 )

• heterozygotes are viable, fertile with no overt abnormalities and normal growth and proliferation of MEFs despite decreased protein levels and S6K1 phosphorylation