Phenotypes associated with this allele

• Allele Symbol: Gabbr1^tm2Bet
• Allele Name: targeted mutation 2, Bernhard Bettler
• Allele ID: MGI:3512742
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gabbr1^tm2Bet/Gabbr1^tm2Bet	BALB/c-Gabbr1^tm2Bet	MGI:3717906
cn2	Gabbr1^tm2Bet/Gabbr1^tm2Bet Hdc^tm1.1(icre)Wwis/Hdc^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:5461822
cn3	Gabbr1^tm2Bet/Gabbr1^tm2Bet Tg(HCRT-cre)1Stak/0	involves: BALB/cA * C57BL/6 * DBA/1	MGI:3839426

Genotype
MGI:3717906

hm1

• Allelic Composition: Gabbr1^tm2Bet/Gabbr1^tm2Bet
• Genetic Background: BALB/c-Gabbr1^tm2Bet

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:94234 )

N • behavior is similar to wild-type littermates

Genotype
MGI:5461822

cn2

• Allelic Composition: Gabbr1^tm2Bet/Gabbr1^tm2Bet; Hdc^{tm1.1(icre)Wwis}/Hdc⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

behavior/neurological

behavior/neurological phenotype ( J:188103 )

N • mice appear indistinguishable from controls in health, behavior and appearance

N • EEG or sleep-wake parameters during the 24 hour cycle do not differ from controls

N • propofol-induced loss of righting reflex (LORR) is not different from controls

abnormal habituation to a new environment ( J:188103 )

• level of arousal (time spent in the wake phase) remains elevated for a longer period in mutants in response to a change of environment, compared to controls

nervous system

abnormal neuron physiology ( J:188103 )

• tuberomammillary nucleus neurons in brain slices from mutant animals are not hyperpolarized by propofol treatment, as observed in control neurons

Genotype
MGI:3839426

cn3

• Allelic Composition: Gabbr1^tm2Bet/Gabbr1^tm2Bet; Tg(HCRT-cre)1Stak/0
• Genetic Background: involves: BALB/cA * C57BL/6 * DBA/1

nervous system

nervous system phenotype ( J:146778 )

N • despite loss of Gabbr1 expression, no decrease in the number of orexin positive neurons is detected and spontaneous excitatory postsynaptic currents in orexin neurons are similar to controls

abnormal CNS synaptic transmission ( J:146778 )

• unlike in controls, treatment with bicuculline induces depolarization of orexin neurons

• membrane resistance of orexin neurons is significantly decreased

• glutamate and GABA-A agonists are less potent in inducing depolarizing currents in orexin neurons compared to controls

abnormal inhibitory postsynaptic currents ( J:146778 )

• increase in the frequency of fast spontaneous inhibitory postsynaptic currents in orexin neurons compared to control littermates

behavior/neurological

abnormal sleep pattern ( J:146778 )

• severe fragmentation of the sleep wake cycle with decreased awake, non-REM, and REM sleep episode durations and reduced REM latency

• fragmentation occurs in both the light and dark phases

• the delta power is significantly increased in non-REM sleep and the theta power is decreased during REM sleep compared to controls

• however, the overall amounts of wakefulness, non-REM and REM sleep are similar to controls