Phenotypes associated with this allele

• Allele Symbol: Pck1^tm1Rwh
• Allele Name: targeted mutation 1, Richard W Hanson
• Allele ID: MGI:3514160
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pck1^tm1Rwh/Pck1^tm1Rwh	involves: 129P2/OlaHsd * C57BL/6	MGI:3833936

Genotype
MGI:3833936

hm1

• Allelic Composition: Pck1^tm1Rwh/Pck1^tm1Rwh
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:145210 )

• born in expected numbers

• no obvious phenotype at birth and suckle normally

• die within 2-3 days of birth

• mortality is not rescued by ip administration of glucose

growth/size/body

abnormal postnatal growth ( J:145210 )

• fail to gain weight by day 1 after birth

adipose tissue

decreased percent body fat/body weight ( J:145210 )

• half the normal level of body fat 1 day after birth

homeostasis/metabolism

abnormal circulating amino acid level ( J:145210 )

• elevated levels of non-essential amino acids in plasma

increased circulating ammonia level ( J:145210 )

• ammonia levels increased 3 fold

increased blood urea nitrogen level ( J:145210 )

• 2 fold increase

decreased circulating glucose level ( J:145210 )

• blood glucose 60% of control levels 1 day after birth

• blood glucose down to 12.3 mg/dl by 3 days after birth

increased circulating ketone body level ( J:145210 )

• beta-hydroxybutyrate elevated 3 fold

increased circulating lactate level ( J:145210 )

• lactate levels increased 2.5 fold

abnormal circulating lipid level ( J:145210 )

increased circulating triglyceride level ( J:145210 )

• 50% increase in triglycerides in blood (36.3 mg/dl vs 23.7 for controls)

decreased liver glycogen level ( J:145210 )

• liver glycogen reduced by 10% one day before birth

• marked reduction of liver glycogen 2 days after birth

liver/biliary system

abnormal liver morphology ( J:145210 )

• liver malate levels increased 10 fold

• liver lactate levels increased 2.5 fold

decreased liver glycogen level ( J:145210 )

• liver glycogen reduced by 10% one day before birth

• marked reduction of liver glycogen 2 days after birth

hepatic steatosis ( J:145210 )

• high levels of lipid in liver

• very high level of triglyceride (9.58 umoles/g vs 4.17 in controls)