All Phenotypes Mgat1<tm2Jxm> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	\C1galt1^tm1.1Rpmc/\C1galt1^tm1.1Rpmc \Mgat1^tm2Jxm/\Mgat1^tm2Jxm \Tg(Zp3-cre)1Gwh/?	involves: 129S1/Sv * 129S1/SvImJ * 129X1/SvJ * C57BL/6	MGI:3714054
cn2	\C1galt1^tm1.1Rpmc/\C1galt1^tm1.1Rpmc \Mgat1^tm2Jxm/\Mgat1^tm2Jxm \Tg(Zp3-cre)1Gwh/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5816473
cn3	\Mgat1^tm2Jxm/\Mgat1^tm2Jxm \Tg(Stra8-icre)1Reb/0	involves: 129S1/Sv * 129X1/SvJ * FVB/NJ	MGI:6888114
cn4	\Mgat1^tm2Jxm/\Mgat1⁺ \Tg(Stra8-icre)1Reb/0	involves: 129S1/Sv * 129X1/SvJ * FVB/NJ	MGI:8213331
cn5	\C1galt1^tm1.1Rpmc/\C1galt1^tm1.1Rpmc \Mgat1^tm2Jxm/\Mgat1^tm2Jxm \Tg(Stra8-icre)1Reb/0	involves: 129S1/Sv * 129X1/SvJ * FVB/NJ	MGI:8213357
cn6	\Mgat1^tm2Jxm/\Mgat1^tm1Pst \Tg(Zp3-cre)1Gwh/0	involves: 129/Sv * C57BL/6 * SJL	MGI:3521575
cn7	\Mgat1^tm2Jxm/\Mgat1^tm2Jxm \Tg(Zp3-cre)1Gwh/0	Not Specified	MGI:3521574

Allelic Composition	\C1galt1^tm1.1Rpmc/\C1galt1^tm1.1Rpmc \Mgat1^tm2Jxm/\Mgat1^tm2Jxm \Tg(Zp3-cre)1Gwh/?
Genetic Background	involves: 129S1/Sv * 129S1/SvImJ * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
C1galt1^tm1.1Rpmc mutation (1 available); any C1galt1 mutation (25 available)
Mgat1^tm2Jxm mutation (1 available); any Mgat1 mutation (17 available)
Tg(Zp3-cre)1Gwh mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

decreased oocyte number ( J:122012 )

• superovulated females produce 50% less oocytes and following the termination of mating females did not produce any oocytes when superovulated

reproductive system

decreased oocyte number ( J:122012 )

• superovulated females produce 50% less oocytes and following the termination of mating females did not produce any oocytes when superovulated

abnormal ovarian follicle morphology ( J:122012 )

• fewer developing follicles in ovaries at 3 to 6 months

reduced female fertility ( J:122012 )

• only 3 of 10 females produced a litter and did not produce any subsequent litters

decreased litter size ( J:122012 )

• females produce smaller litters

endocrine/exocrine glands

abnormal ovarian follicle morphology ( J:122012 )

• fewer developing follicles in ovaries at 3 to 6 months

Allelic Composition	\C1galt1^tm1.1Rpmc/\C1galt1^tm1.1Rpmc \Mgat1^tm2Jxm/\Mgat1^tm2Jxm \Tg(Zp3-cre)1Gwh/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature ovarian failure ( J:173879 )

• females exhibit premature loss of fertility

reproductive system

abnormal zona pellucida morphology ( J:173879 )

• some oocytes within multiple-oocyte follicles at 3 weeks of age lack a zona pollucida and associated granulosa cells

abnormal ovary morphology ( J:173879 )

• stromal cells of 3 month old ovaries are luteinized and look more like the cells of corpus lutea than stroma

abnormal corpus luteum morphology ( J:173879 )

• the majority of ovaries lack normal corpora lutea at 3 months

abnormal ovarian follicle morphology ( J:173879 )

• joined follicles are present in 3 week old ovaries, as indicated by the fold of basal lamina and theca cells that partially separates two connected follicles

• by 6 weeks of age, females have more follicular abnormalities and have fewer follicles

• numerous oocytes lacking cumulus cells are seen in follicles at 6 weeks of age

abnormal granulosa cell morphology ( J:173879 )

• some oocytes within multiple-oocyte follicles at 3 weeks of age lack a zona pollucida and associated granulosa cells

• oocytes that lack associated granulosa cells but have a zona pellucida are also seen at 6 weeks of age

• granulosa cells are visible beneath the oocyte zona pellucida in follicles at 3 weeks of age, representing either abnormal follicle development or granulosa cell invasion

abnormal ovarian follicle number ( J:173879 )

• by 3 months of age, ovaries contain fewer growing follicles, with fewer secondary, preantral, and antral follicles

• however, the total number of follicles is similar to controls, due to increased numbers of primary follicles at the 3a stage

increased primary ovarian follicle number ( J:173879 )

• increase in numbers of primary follicles at the 3a stage

abnormal ovarian folliculogenesis ( J:173879 )

• ovaries have decreased follicle development at 3 months of age

abnormal tertiary ovarian follicle morphology ( J:173879 )

• large antral follicles at 6 weeks have an irregular follicle boundary and contain granulosa cells with an asymmetric distribution

decreased tertiary ovarian follicle number ( J:173879 )

• fewer antral follicles in ovaries at 3 months of age

polyovular ovarian follicle ( J:173879 )

• ovaries of 3 week old mice contain multiple-oocyte follicles

decreased ovary weight ( J:173879 )

• at 3 months of age

abnormal ovary secretion ( J:173879 )

• ovaries from 3 month old mice contain abnormal leuteinized-like cells that express 3beta-HSD, the enzyme required to convert pregnenalone to progesterone, indicating that these cells secrete progesterone

premature ovarian failure ( J:173879 )

• females exhibit premature loss of fertility

decreased superovulation rate ( J:173879 )

• at 7 weeks of age, the number of eggs ovulated by females in response to the superovulatory stimulus is less than 10% of controls and by 11 week, the ovulation rate falls to less than 1%

• ovulation of eggs is restricted to the period before 3 months of age

female infertility ( J:173879 )

• females are infertile at 3 months of age

reduced female fertility ( J:173879 )

• females exhibit decreased fertility at 6 weeks and are infertile at 3 months of age

endocrine/exocrine glands

abnormal ovary morphology ( J:173879 )

• stromal cells of 3 month old ovaries are luteinized and look more like the cells of corpus lutea than stroma

abnormal corpus luteum morphology ( J:173879 )

• the majority of ovaries lack normal corpora lutea at 3 months

abnormal ovarian follicle morphology ( J:173879 )

• joined follicles are present in 3 week old ovaries, as indicated by the fold of basal lamina and theca cells that partially separates two connected follicles

• by 6 weeks of age, females have more follicular abnormalities and have fewer follicles

• numerous oocytes lacking cumulus cells are seen in follicles at 6 weeks of age

abnormal granulosa cell morphology ( J:173879 )

• some oocytes within multiple-oocyte follicles at 3 weeks of age lack a zona pollucida and associated granulosa cells

• oocytes that lack associated granulosa cells but have a zona pellucida are also seen at 6 weeks of age

• granulosa cells are visible beneath the oocyte zona pellucida in follicles at 3 weeks of age, representing either abnormal follicle development or granulosa cell invasion

abnormal ovarian follicle number ( J:173879 )

• by 3 months of age, ovaries contain fewer growing follicles, with fewer secondary, preantral, and antral follicles

• however, the total number of follicles is similar to controls, due to increased numbers of primary follicles at the 3a stage

increased primary ovarian follicle number ( J:173879 )

• increase in numbers of primary follicles at the 3a stage

abnormal ovarian folliculogenesis ( J:173879 )

• ovaries have decreased follicle development at 3 months of age

abnormal tertiary ovarian follicle morphology ( J:173879 )

• large antral follicles at 6 weeks have an irregular follicle boundary and contain granulosa cells with an asymmetric distribution

decreased tertiary ovarian follicle number ( J:173879 )

• fewer antral follicles in ovaries at 3 months of age

polyovular ovarian follicle ( J:173879 )

• ovaries of 3 week old mice contain multiple-oocyte follicles

decreased ovary weight ( J:173879 )

• at 3 months of age

abnormal ovary secretion ( J:173879 )

premature ovarian failure ( J:173879 )

• females exhibit premature loss of fertility

homeostasis/metabolism

abnormal hormone level ( J:173879 )

• decrease in circulating inhibin A levels

decreased circulating testosterone level ( J:173879 )

• testosterone levels are decreased

• however, estradiol, progesterone, and leuteinizing hormone levels are normal

increased circulating follicle stimulating hormone level ( J:173879 )

cellular

abnormal zona pellucida morphology ( J:173879 )

• some oocytes within multiple-oocyte follicles at 3 weeks of age lack a zona pollucida and associated granulosa cells

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

abnormal male germ cell morphology ( J:185823 )

• at 7 weeks of age, testis sections show essentially no binding of the plant lectins Phaseolus vulgaris leucoagglutinin (L-PHA) and Griffonia simplicifolia agglutinin (GSA) to male germ cells, indicating the absence of complex N-glycans on germ cell glycoproteins

• in contrast, male germ cells in all tubules show binding of concanavalin A (Con A), a lectin that binds preferentially to high-mannose N-glycans

multinucleated giant male germ cells ( J:185823 , J:260130 )

• at 7 weeks of age, all seminiferous tubules contain multinucleated giant cells (MNCs) or symplasts in the region normally occupied by spermatids; MNCs are composed of either round or elongating spermatids, suggesting that germ differentiation proceeds to some extent (J:185823)

• at 22 weeks of age, small MNCs are occasionally detected in the epididymides (J:185823)

• at 24 and 25 dpp, fusion of cells adjacent to the lumen is observed in a few tubules (J:260130)

• at 28 dpp, ~14% of tubules contain multinucleated cells composed of fused spermatids (J:260130)

increased male germ cell apoptosis ( J:185823 )

• at 7 weeks of age, the % of tubules containing apoptotic cells is ~90% versus ~25% in control males, as determined by a TUNEL assay; the total number of apoptotic cells per TUNEL+ tubule is increased by ~3.5-fold

• however, parenchymal cells outside the tubules appear unaffected

abnormal seminiferous tubule morphology ( J:185823 )

• at 7 weeks of age, seminiferous tubule architecture is severely disrupted, with all tubules containing multinucleated giant male germ cells

decreased testis weight ( J:185823 )

• at 7 weeks of age, testis weight is reduced by ~33% relative to that in control males

• testis-to-body weight ratio is significantly decreased at both 7 and 22 weeks of age

abnormal testis physiology ( J:185823 )

• decapsulated testis extracts are completely sensitive to endoglycosidase H (Endo H), an enzyme that cleaves only high-mannose and hybrid N-glycans, but not complex N-glycans, indicating that male germ cells produce no detectable complex N-glycans

abnormal spermatogenesis ( J:185823 )

azoospermia ( J:185823 , J:260130 )

• 7-week-old males are unable to produce sperm; epididymides are completely devoid of spermatozoa at 22 weeks of age (J:185823)

• at 28 dpp, no mature spermatozoa are present in testis sections (J:260130)

decreased elongated spermatid number ( J:260130 )

• at 25 dpp, few elongated spermatids are observed

arrest of spermatogenesis ( J:260130 )

• starting at 24 and 25 dpp, fusion of cells adjacent to the lumen is observed in a few tubules

• at 28 dpp, number of tubules with elongated spermatids is significantly decreased and multinucleated germ cells, but no mature spermatozoa, are present

• premature upregulation of genes normally expressed later in spermatogenesis and spermiogenesis is observed at 22 and 23 dpp

• EGFR and PDGFRA transcripts and ERK1/2 signaling are decreased in 22 dpp male germ cells

• however, the number of Sertoli cells, spermatogonia, spermatocytes and stage VI and beyond tubules are relatively normal at 24-26 dpp

abnormal epididymis morphology ( J:185823 )

• at 22 weeks of age, epididymides are completely devoid of spermatozoa but occasionally contain small multinucleated giant cells (MNCs) or aberrant spermatids

• degenerate germ cells accumulate in the epididymides of adult males

male infertility ( J:185823 , J:260130 )

• all males fail to generate progeny after mating with two or more wild-type females (J:185823)

(J:260130)

growth/size/body

decreased body weight ( J:185823 )

• at 22 weeks of age, male body weight is significantly lower than in control males

• however, male body weight is normal at 7 weeks of age

cellular

abnormal male germ cell morphology ( J:185823 )

• in contrast, male germ cells in all tubules show binding of concanavalin A (Con A), a lectin that binds preferentially to high-mannose N-glycans

azoospermia ( J:185823 , J:260130 )

• 7-week-old males are unable to produce sperm; epididymides are completely devoid of spermatozoa at 22 weeks of age (J:185823)

• at 28 dpp, no mature spermatozoa are present in testis sections (J:260130)

decreased elongated spermatid number ( J:260130 )

• at 25 dpp, few elongated spermatids are observed

multinucleated giant male germ cells ( J:185823 , J:260130 )

• at 22 weeks of age, small MNCs are occasionally detected in the epididymides (J:185823)

• at 24 and 25 dpp, fusion of cells adjacent to the lumen is observed in a few tubules (J:260130)

• at 28 dpp, ~14% of tubules contain multinucleated cells composed of fused spermatids (J:260130)

increased male germ cell apoptosis ( J:185823 )

• however, parenchymal cells outside the tubules appear unaffected

endocrine/exocrine glands

abnormal seminiferous tubule morphology ( J:185823 )

• at 7 weeks of age, seminiferous tubule architecture is severely disrupted, with all tubules containing multinucleated giant male germ cells

decreased testis weight ( J:185823 )

• at 7 weeks of age, testis weight is reduced by ~33% relative to that in control males

• testis-to-body weight ratio is significantly decreased at both 7 and 22 weeks of age

abnormal testis physiology ( J:185823 )

Allelic Composition	\Mgat1^tm2Jxm/\Mgat1⁺ \Tg(Stra8-icre)1Reb/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * FVB/NJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mgat1^tm2Jxm mutation (1 available); any Mgat1 mutation (17 available)
Tg(Stra8-icre)1Reb mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

reproductive system phenotype ( J:185823 )

N	• male mice exhibit normal fertility and spermatogenesis with no differences in testicular and epididymal histology, intratubular structure or germ cell distribution relative to control males; epididymal ducts are full of sperm

Allelic Composition	\C1galt1^tm1.1Rpmc/\C1galt1^tm1.1Rpmc \Mgat1^tm2Jxm/\Mgat1^tm2Jxm \Tg(Stra8-icre)1Reb/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * FVB/NJ

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

multinucleated giant male germ cells ( J:185823 )

abnormal seminiferous tubule morphology ( J:185823 )

• male mice show the same testicular defects observed in sections from Mgat1^tm2Jxm/Mgat1^tm2Jxm> Tg(Stra8-icre)1Reb/0 males (MGI:6888114)

abnormal spermatogenesis ( J:185823 )

azoospermia ( J:185823 )

abnormal epididymis morphology ( J:185823 )

• male mice show the same epididymal defects observed in sections from Mgat1^tm2Jxm/Mgat1^tm2Jxm> Tg(Stra8-icre)1Reb/0 males (MGI:6888114)

male infertility ( J:185823 )

cellular

azoospermia ( J:185823 )

multinucleated giant male germ cells ( J:185823 )

endocrine/exocrine glands

abnormal seminiferous tubule morphology ( J:185823 )

• male mice show the same testicular defects observed in sections from Mgat1^tm2Jxm/Mgat1^tm2Jxm> Tg(Stra8-icre)1Reb/0 males (MGI:6888114)

Allelic Composition	\Mgat1^tm2Jxm/\Mgat1^tm1Pst \Tg(Zp3-cre)1Gwh/0
Genetic Background	involves: 129/Sv * C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mgat1^tm1Pst mutation (0 available); any Mgat1 mutation (17 available)
Mgat1^tm2Jxm mutation (1 available); any Mgat1 mutation (17 available)
Tg(Zp3-cre)1Gwh mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

abnormal oogenesis ( J:94224 )

• the number of eggs obtained from mutant females is about 50% that from control females lacking Tg(Zp3-cre)1Gwh and more mutant females failed to produce any eggs after superovulation

abnormal zona pellucida morphology ( J:94224 )

• the zona pellucida is thinner, less rigid, more loosely attached to the egg, lacks complex N glycans, and is stickier, however mutant eggs can still be successfully fertilized

thin zona pellucida ( J:94224 )

reduced female fertility ( J:94224 )

• mutant females crossed to heterozygous or wild-type males have more abnormal or underdeveloped embryos compared to control females

• 41% of mutant females fail to produce any embryos within 5 days of their first mating compared to about 12% of control females

decreased litter size ( J:94224 )

• average litter size for mutant females mated to wild-type males is only 3.8 pups compared to 7.1 pups for wild-type females

cellular

abnormal oogenesis ( J:94224 )

• the number of eggs obtained from mutant females is about 50% that from control females lacking Tg(Zp3-cre)1Gwh and more mutant females failed to produce any eggs after superovulation

abnormal zona pellucida morphology ( J:94224 )

• the zona pellucida is thinner, less rigid, more loosely attached to the egg, lacks complex N glycans, and is stickier, however mutant eggs can still be successfully fertilized

thin zona pellucida ( J:94224 )

Allelic Composition	\Mgat1^tm2Jxm/\Mgat1^tm2Jxm \Tg(Zp3-cre)1Gwh/0
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mgat1^tm2Jxm mutation (1 available); any Mgat1 mutation (17 available)
Tg(Zp3-cre)1Gwh mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

abnormal oogenesis ( J:94224 )

• the number of eggs obtained from mutant females is about 50% that from control females lacking Tg(Zp3-cre)1Gwh and more mutant females failed to produce any eggs after superovulation

abnormal zona pellucida morphology ( J:94224 )

• the zona pellucida is thinner, less rigid, more loosely attached to the egg, lacks complex N glycans, and is stickier, however mutant eggs can still be successfully fertilized

thin zona pellucida ( J:94224 )

reduced female fertility ( J:94224 )

• mutant females crossed to heterozygous or wild-type males have more abnormal or underdeveloped embryos compared to control females

• 41% of mutant females fail to produce any embryos within 5 days of their first mating compared to about 12% of control females

decreased litter size ( J:94224 )

• average litter size for mutant females mated to wild-type males is only 3.8 pups compared to 7.1 pups for wild-type females

cellular

abnormal oogenesis ( J:94224 )

• the number of eggs obtained from mutant females is about 50% that from control females lacking Tg(Zp3-cre)1Gwh and more mutant females failed to produce any eggs after superovulation

abnormal zona pellucida morphology ( J:94224 )

• the zona pellucida is thinner, less rigid, more loosely attached to the egg, lacks complex N glycans, and is stickier, however mutant eggs can still be successfully fertilized

thin zona pellucida ( J:94224 )

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