Phenotypes associated with this allele

• Allele Symbol: Tg(KRT14-HPV16E7)2304Plam
• Allele Name: transgene insertion 2304, Paul F Lambert
• Allele ID: MGI:3514313
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rb1^tm3Tyj/Rb1^tm3Tyj Tg(KRT14-cre)8Brn/0 Tg(KRT14-HPV16E7)2304Plam/0	involves: 129S4/SvJae * C57BL/6 * FVB	MGI:3607134
cx2	Rb1^tm1Fad/Rb1^tm1Fad Tg(KRT14-HPV16E7)2304Plam/0	involves: 129 * C57BL/6 * FVB	MGI:3607130
cx3	Rb1^tm1Fad/Rb1^+ Tg(KRT14-HPV16E7)2304Plam/0	involves: 129 * C57BL/6 * FVB	MGI:3607131
cx4	Rb1^tm3Tyj/Rb1^tm3Tyj Tg(KRT14-HPV16E7)2304Plam/0	involves: 129S4/SvJae * C57BL/6 * FVB	MGI:3607135
tg5	Tg(KRT14-HPV16E7)2304Plam/Tg(KRT14-HPV16E7)2304Plam	involves: FVB/N	MGI:3606961
tg6	Tg(KRT14-HPV16E7)2304Plam/0	involves: FVB/N	MGI:3606960

Genotype
MGI:3607134

cn1

• Allelic Composition: Rb1^tm3Tyj/Rb1^tm3Tyj; Tg(KRT14-cre)8Brn/0; Tg(KRT14-HPV16E7)2304Plam/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

integument

abnormal epidermis stratum granulosum morphology ( J:101623 )

• thickness of the granular layer is increased to a greater extent than in conditional RB1 mice that lack Tg(KRT14-HPV16E7)2304Plam

abnormal epidermis suprabasal layer morphology ( J:101623 )

• thickness of the spinous suprabasal layer is increased

epidermal hyperplasia ( J:101623 )

• epidermal hyperplasia is increased compared to conditional RB1 mice that lack Tg(KRT14-HPV16E7)2304Plam

Genotype
MGI:3607130

cx2

• Allelic Composition: Rb1^tm1Fad/Rb1^tm1Fad; Tg(KRT14-HPV16E7)2304Plam/0
• Genetic Background: involves: 129 * C57BL/6 * FVB

cellular

cellular phenotype ( J:101623 )

N • after exposure to 5 Gy of ionizing radiation skin cells display normal cell cycle arrest unlike in mice hemizygous for Tg(KRT14-HPV16E7)2304Plam alone

integument

abnormal epidermis suprabasal layer morphology ( J:101623 )

• thickness of the spinous suprabasal cell layer is increased 30-50%; however, the thickness of the keratin 10- and filaggrin-positive layers is normal

• mice have normal gross skin morphology, normal growth and no signs of epidermal hyperplasia unlike in mice hemizygous for Tg(KRT14-HPV16E7)2304Plam alone

Genotype
MGI:3607131

cx3

• Allelic Composition: Rb1^tm1Fad/Rb1⁺; Tg(KRT14-HPV16E7)2304Plam/0
• Genetic Background: involves: 129 * C57BL/6 * FVB

cellular

cellular phenotype ( J:101623 )

N • after exposure to 5 Gy of ionizing radiation skin cells display normal cell cycle arrest unlike in mice hemizygous for Tg(KRT14-HPV16E7)2304Plam alone

integument

abnormal epidermis suprabasal layer morphology ( J:101623 )

• thickness of the spinous suprabasal cell layer is increased 30-50%; however, the thickness of the keratin 10- and filaggrin-positive layers is normal

• mice have normal gross skin morphology, normal growth and no signs of epidermal hyperplasia unlike in mice hemizygous for Tg(KRT14-HPV16E7)2304Plam alone

Genotype
MGI:3607135

cx4

• Allelic Composition: Rb1^tm3Tyj/Rb1^tm3Tyj; Tg(KRT14-HPV16E7)2304Plam/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * FVB

integument

abnormal epidermis stratum granulosum morphology ( J:101623 )

• thickness of the granular layer is increased to a greater extent than in conditional RB1 mice that lack Tg(KRT14-HPV16E7)2304Plam

abnormal epidermis suprabasal layer morphology ( J:101623 )

• thickness of the spinous suprabasal layer is increased

Genotype
MGI:3606961

tg5

• Allelic Composition: Tg(KRT14-HPV16E7)2304Plam/Tg(KRT14-HPV16E7)2304Plam
• Genetic Background: involves: FVB/N

vision/eye

developmental cataract ( J:86018 )

• small bilateral cataracts are seen at birth

growth/size/body

postnatal growth retardation ( J:86018 )

• a low penetrance of growth stunting is seen

• this stunting is not as severe as in Tg(KRT14-HPV16E7)2350Plam hemizygous mice

Genotype
MGI:3606960

tg6

• Allelic Composition: Tg(KRT14-HPV16E7)2304Plam/0
• Genetic Background: involves: FVB/N

neoplasm

increased skin squamous cell carcinoma incidence ( J:86018 )

• beginning around 9 months of age, small highly keratotic lesions representing low-grade squamous cell carcinomas are seen in the skin

growth/size/body

epithelioid cyst ( J:86018 )

• beginning around 9 months of age, large, cystic but nonkeratotic lesions representing aggressive sebaceous epitheliomas and containing large accumulations of sebum are seen in the skin

vision/eye

developmental cataract ( J:86018 )

• small bilateral cataracts are seen at birth

digestive/alimentary system

abnormal esophageal squamous epithelium morphology ( J:86018 )

• the thickness of the nucleated layer is increased in squamous epithelia in the upper digestive tract

abnormal stomach non-glandular epithelium morphology ( J:86018 )

• squamous epithelia in the forestomach is highly invaginated

cellular

abnormal cell cycle checkpoint function ( J:101623 )

• after exposure to 5 Gy of ionizing radiation skin cells do not display normal cell cycle arrest

increased hair follicle apoptosis ( J:86018 )

• an increase in apoptotic cells is seen in hair follicles

integument

increased hair follicle apoptosis ( J:86018 )

• an increase in apoptotic cells is seen in hair follicles

epithelioid cyst ( J:86018 )

• beginning around 9 months of age, large, cystic but nonkeratotic lesions representing aggressive sebaceous epitheliomas and containing large accumulations of sebum are seen in the skin

sparse hair ( J:86018 )

• mice appear scruffy with less dense fur

matted coat ( J:86018 )

• the fur often appears greasy and matted

greasy coat ( J:86018 )

• the fur often appears greasy and matted

abnormal epidermal layer morphology ( J:86018 , J:101623 )

• about a 5-fold increase in apoptosis primarily in the stratum granulosum and these apoptotic cells occur in patches (J:86018)

• squamous epithelia in the skin is highly invaginated (J:86018)

• the pattern of differentiation in the epidermis is disrupted with expansion of the keratin 10- and filaggrin-positive layers (J:101623)

epidermal hyperplasia ( J:86018 )

• clearly seen in adult ears

• a disorganized pattern of proliferating cells is seen in the skin with many proliferating cells that are not in contact with the basement membrane present

• hyperproliferation of epithelial cells results in lateral and vertical expansion of the affected epithelium

wrinkled skin ( J:86018 )

• first seen at P3 in most mice

thick skin ( J:86018 )

• the skin on the ears and/or snout is thickened in neonatal and adult mice

increased skin squamous cell carcinoma incidence ( J:86018 )

• beginning around 9 months of age, small highly keratotic lesions representing low-grade squamous cell carcinomas are seen in the skin