All Phenotypes Cst8<tm1Itl> MGI Mouse

reproductive system phenotype ( J:168157 )

N	• in vivo, both male and female homozygous mutant mice generate litters of normal litter size and, thus, exhibit normal fertility

decreased sperm progressive motility ( J:168157 )

• spermatozoa show a small but significant decrease (~10%) in the percentage of spermatozoa moving forward at 30, 60 and 120 min after capacitation

asthenozoospermia ( J:168157 )

• spermatozoa show a small but significant decrease (~10%) in the percentage of motile spermatozoa at 30, 60 and 120 min after capacitation

• however, track speed, path velocity, progressive velocity, lateral displacement, beat cross frequency, straightness, and linearity are normal

decreased testis weight ( J:168157 )

• testis/body weight ratio is significantly decreased at 17-21 weeks but not at 12-16 weeks of age

abnormal spermatogenesis ( J:168157 )

• preliminary studies showed that, in some seminiferous tubules, germ cell-Sertoli cell interactions are disrupted resulting in premature sloughing of germ cells

oligozoospermia ( J:168157 )

• number of cauda epididymidal spermatozoa is significantly decreased both at 12-16 and at 17-21 weeks of age

impaired sperm capacitation ( J:168157 )

• spermatozoa show a limited ability for protein tyrosine phosphorylation during capacitation

• incubation of spermatozoa with dibutyryl cAMP and 3-isobutyl-1-methylxanthine (IBMX) rescues the capacity for sperm protein tyrosine phosphorylation

• however, untreated spermatozoa show a normal increase in total levels of cAMP and protein kinase A (PKA) activity throughout the capacitation time course relative to spermatozoa incubated under non-capacitating conditions

decreased epididymis weight ( J:168157 )

• epididymis/body weight ratio is significantly decreased at 12-16 weeks but not at 17-21 weeks of age

impaired acrosome reaction ( J:168157 )

• after 60 and 90 min of capacitation, the percentage of spermatozoa undergoing a progesterone-stimulated acrosome reaction is significantly lower than that in wild-type spermatozoa

• however, no differences in A23187-induced acrosome reaction are observed

abnormal sperm-egg fusion ( J:168157 )

• in vitro, ability of spermatozoa to fuse with the egg plasma membrane is reduced by 68%

decreased fertilization frequency ( J:168157 )

• in vitro, spermatozoa show a 72% decrease in fertilizing efficiency: only 19% of cumulus-oocyte complexes (COCs) are fertilized relative to 66% of COCs fertilized by wild-type spermatozoa

• incubation of spermatozoa with dibutyryl cAMP and 3-isobutyl-1-methylxanthine (IBMX) during capacitation rescues the fertilization defect

impaired binding of sperm to zona pellucida ( J:168157 )

• in vitro, ability of spermatozoa to bind to the egg zona pellucida is reduced by 53%