Phenotypes associated with this allele

• Allele Symbol: Nr2f1^tm1Mist
• Allele Name: targeted mutation 1, Michele Studer
• Allele ID: MGI:3521756
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nr2f1^tm1Mist/Nr2f1^tm1Mist	involves: 129S2/SvPas * C57BL/6J	MGI:3688750

Genotype
MGI:3688750

hm1

• Allelic Composition: Nr2f1^tm1Mist/Nr2f1^tm1Mist
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:114098 )

• all homozygotes die at perinatal stages

nervous system

abnormal brain commissure morphology ( J:114098 )

• corpus callosum and hippocampal commissure are stunted at midline

abnormal corpus callosum morphology ( J:114098 )

• at E18.5, callosal projections in mutants fail to show decussation in most fetuses

• non-crossing commissural fibers have an abnormal defasciculated appearance

• at all levels, callosal projections form aberrantly oriented Probst bundles

• mutants fail to show segregation of anterior versus posterior projections

abnormal hippocampal commissure morphology ( J:114098 )

• failure of hippocampal commissure to cross midline is observed and ventrally oriented ectopic thick bundles are seen

• all hippocampal fibers form a thick bundle together with the fornix which shows aberrant growth into the septal region

abnormal anterior commissure morphology ( J:114098 )

• number of anterior limb branches of the anterior commissure (AC) in the olfactory cortex is increased

• anterior and posterior branching are abnormally positioned dorsoventrally; innervation of anteromedial cortex by these branches is through numerous tracts

• some AC fascicles show aberrant connections with hippocampal commissure

abnormal cerebral cortex morphology ( J:114098 )

• embryos present a significantly extended length of medial cortex compared with dorsoventral length of the telencephalon

abnormal cingulate gyrus morphology ( J:114098 )

• embryos present a ventrally extended cingulate cortex

abnormal neuron morphology ( J:114098 )

• cultured hippocampal neurons from mutants have no protrusions or a slightly elongated process after 12 hours

• when normal neurons show a symmetrical distribution of neurites, mutant neurons have no processes or abnormal neurites that tended to curl up on themselves

abnormal axon guidance ( J:114098 )

• all major forebrain commissures have guidance abnormalities along anterior-posterior and dorsoventral axesin mutants

abnormal axon morphology ( J:114098 )

• cultured neurons display numerous short filopodial extensions and growth cone-like structures around the cell body and neurites; axons roll up in an abnormal fashion

• Map1b protein levels in growing axons are decreased significantly

cellular

abnormal axon guidance ( J:114098 )

• all major forebrain commissures have guidance abnormalities along anterior-posterior and dorsoventral axesin mutants