Analysis Tools
immune system
| N |
• spleens and Peyer's patches are normal in size, and show germinal centers of normal morphologies; numbers of IgM+, B220+ B cells in peripheral compartments are normal
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• mutants exhibit a severe CSR defect; the defect slightly affects IgM and IgG1 levels, whereas IgG2b and IgG3 are significantly affected both in vivo and in vitro, with IgG2a amd IgE classes are moderately affected
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• LPS + TGF beta-stimulated IgA production in vitro is lowered ~3-fold
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• LPS + Il-4-stimulated IgE production in vitro is decreased ~10-fold compared to wild-type; in most experiments, levels are below detection limits; in vivo, levels are reduced 4-fold
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• LPS-stimulated B cells in vitro secrete 15-fold less IgG2b and 100-fold less IgG3 than wild-type cells; in vivo, IgG2b levels are decrased 10-fold and IgG3 is 10-fold reduced
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• LPS + IFN gamma-stimulated IgG2a secretion in vitro is decreased ~10-fold; in vivo, levels are reduced 10-fold
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hematopoietic system
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• mutants exhibit a severe CSR defect; the defect slightly affects IgM and IgG1 levels, whereas IgG2b and IgG3 are significantly affected both in vivo and in vitro, with IgG2a amd IgE classes are moderately affected
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• LPS + TGF beta-stimulated IgA production in vitro is lowered ~3-fold
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• LPS + Il-4-stimulated IgE production in vitro is decreased ~10-fold compared to wild-type; in most experiments, levels are below detection limits; in vivo, levels are reduced 4-fold
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• LPS-stimulated B cells in vitro secrete 15-fold less IgG2b and 100-fold less IgG3 than wild-type cells; in vivo, IgG2b levels are decrased 10-fold and IgG3 is 10-fold reduced
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• LPS + IFN gamma-stimulated IgG2a secretion in vitro is decreased ~10-fold; in vivo, levels are reduced 10-fold
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