All Phenotypes Afdn<tm1Mrcr> MGI Mouse

embryonic lethality during organogenesis, complete penetrance ( J:57046 )

• homozygotes die around E10

abnormal germ layer development ( J:57046 )

abnormal ectoderm development ( J:57046 )

• at E7.5 the ectoderm at the midline which will become neuroepithelium appears more stratified and less columnar

abnormal mesoderm development ( J:57046 )

• the mesoderm is less dense

abnormal rostral-caudal body axis extension ( J:57046 )

• posterior body elongation is retarded at E7.5

embryonic growth arrest ( J:57046 )

• at E9.5 embryos appear comma-shaped, lack recognizable structures, and consist mostly of loose mesenchyme

decreased embryo size ( J:57046 )

• at E7.5 mutants are smaller than wild-type littermates with a relative decrease in the size of the embryonic tissue relative to the extraembryonic tissue

absent neural folds ( J:57046 )

• the neural folds are absent at E7.5

abnormal embryonic neuroepithelium morphology ( J:57046 )

• by E8.0-E8.5 the presumptive neuroectodermal layer is thick and disorganized while the anterior and posterior margins of the layer are thin

• at E7.5 the neuroepithelium is present but poorly developed at the anterior and posterior end of the embryo

• at E7.5 gaps are present between ectodermal cells of the lumenal layer, no identifiable tight or adherens cell junctions are found, and neuroectodermal cell polarity is reduced

absent notochord ( J:57046 )

• at E8.5 the notochord is absent

absent somites ( J:57046 )

• at E8.5 somites are absent

absent allantois ( J:57046 )

• no connection is formed between the embryonic and maternal circulations