Phenotypes associated with this allele

• Allele Symbol: Chrna2^tm1Jbou
• Allele Name: targeted mutation 1, Jim Boulter
• Allele ID: MGI:3526149
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Chrna2^tm1Jbou/Chrna2^tm1Jbou	B6.129X1-Chrna2^tm1Jbou	MGI:3790875

Genotype
MGI:3790875

hm1

• Allelic Composition: Chrna2^tm1Jbou/Chrna2^tm1Jbou
• Genetic Background: B6.129X1-Chrna2^tm1Jbou

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:126075 )

N • in mutant mice, basal synaptic transmission and short-term synaptic plasticity, measured by recording fEPSPs and PPF, is similar to controls

N • conventional theta burst stimulation (TBS) induces robust and stable LTP in Schaffer collateral CA1 synapses, similar to controls

abnormal excitatory postsynaptic currents ( J:126075 )

• in mutant mice, nicotine does not induce nor promote the spread of excitatory neural activity from the Schaffer collateral pathway as measured by fEPSPs and optical fluorescence

abnormal inhibitory postsynaptic currents ( J:126075 )

• in mutant mice, nicotine does not promote the spread of inhibitory neural activity from the temporoammonic pathway as measured by fEPSPs and optical fluorescence

abnormal long-term potentiation ( J:126075 )

• in Schaffer collateral CA1 synapses, nicotine induced facilitation of theta-burst stimulated LTP induction is supressed

• in temporoammonic CA1 synapses, nicotine supression of LTP induction is eliminated

behavior/neurological

behavior/neurological phenotype ( J:272596 )

♂N • normal stereotypical behavior in contextual fear conditioning chamber on preexposure day 1 when treated with either saline or nicotine

♂N • normal rearing behavior in contextual fear conditioning chamber on preexposure day 1 when treated with either saline or nicotine

♂N • normal time spent in center and perimeter in contextual fear conditioning chamber on preexposure day 1 when treated with either saline or nicotine

♂N • normal behavior in open field test when treated with either saline or nicotine

♂N • normal shock reactivity on test day 2 after preexposure on day 1 when treated with either saline or nicotine

impaired contextual conditioning behavior ( J:272596 )

♂ • no enhanced freezing behavior on test day 2 after preexposure on day 1 when treated with nicotine or on day 3 when treated with either saline or nicotine

♂ • normal (enhanced) freezing behavior on test day 2 after preexposure on day 1 when treated with saline

increased locomotor activity ( J:272596 )

♂ • increased mid-line crossing in contextual fear conditioning chamber on preexposure day 1 when treated with nicotine or saline

♂ • increased locomotor activity in contextual fear conditioning chamber on preexposure day 1 when treated with either saline or nicotine

♂ • normal mid-line crossing in contextual fear conditioning chamber on preexposure day 1 when treated with saline

homeostasis/metabolism

decreased physiological sensitivity to xenobiotic ( J:126075 )

• in mutant mice, nicotine fails to facilitate LTP in the Schaffer collateral pathway and fails to supress LTP induction in the temporoammonic pathway

• nicotine fails to induce and promote the spread of excitatory activity in the Schaffer collateral pathway and fails to supress inhibitory activity in the temporoammonic pathway

embryo

embryo phenotype ( J:272596 )

♂N • mice born at expected Mendelian and sex ratios

growth/size/body

growth/size/body region phenotype ( J:272596 )

♂N • normal postnatal weight gain

♂N • no obvious morphological changes