Phenotypes associated with this allele

• Allele Symbol: Wnt7b^tm2Amc
• Allele Name: targeted mutation 2, Andrew P McMahon
• Allele ID: MGI:3526432
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Twist2^tm1(cre)Dor/Twist2^+ Wnt7b^tm1Parr/Wnt7b^tm2Amc	involves: 129S1/Sv * 129X1/SvJ	MGI:3841730
cn2	Edil3^Tg(Sox2-cre)1Amc/Edil3^+ Wnt7a^tm1Amc/Wnt7a^tm1Amc Wnt7b^tm1Parr/Wnt7b^tm2Amc	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA	MGI:3831189
cn3	Tg(Nes-cre)1Kln/0 Wnt7a^tm1Amc/Wnt7a^tm1Amc Wnt7b^tm1Parr/Wnt7b^tm2Amc	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:3831188
cn4	Gt(ROSA)26Sor^tm1Sor/? Wnt7b^tm2Amc/Wnt7b^tm2.1Amc Tg(Hoxb7-cre)13Amc/0	involves: 129S4/SvJaeSor * 129X1/SvJ * C57BL/6	MGI:3829882
cn5	Wnt7b^tm2Amc/Wnt7b^tm2.1Amc Tg(Hoxb7-cre)13Amc/0	involves: 129X1/SvJ * C57BL/6	MGI:3829881
cn6	Wnt7b^tm2Amc/Wnt7b^tm2.1Amc Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129X1/SvJ * C57BL/6 * CBA	MGI:3793499

Genotype
MGI:3841730

cn1

• Allelic Composition: Twist2^tm1(cre)Dor/Twist2⁺; Wnt7b^tm1Parr/Wnt7b^tm2Amc
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

abnormal osteoblast differentiation ( J:117333 )

• calvaria cells from neonates and bone marrow stromal cells from adults produce fewer bone nodules than wild-type cells

decreased width of hypertrophic chondrocyte zone ( J:117333 )

• at E15.5

abnormal bone ossification ( J:117333 )

• at E15.5, ossification if diminished compared to in wild-type mice

• bone collars of long bones are shorter than in wild-type mice

• at E18.5, skulls exhibit decreased ossification compared to in wild-type mice

cellular

abnormal osteoblast differentiation ( J:117333 )

• calvaria cells from neonates and bone marrow stromal cells from adults produce fewer bone nodules than wild-type cells

Genotype
MGI:3831189

cn2

• Allelic Composition: Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺; Wnt7a^tm1Amc/Wnt7a^tm1Amc; Wnt7b^tm1Parr/Wnt7b^tm2Amc
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:142352 )

• all embryos die around E12.5 due to severe hemorrhaging within the central nervous system

cardiovascular system

abnormal vasculogenesis ( J:142352 )

• while vascularization has occurred within the ventral neural tube of control E10.5 embryos at the forelimb level, no such vascularization is observed in this region of mutant embryos

• in E12.5 embryos, endothelial cells and pericytes are absent from all ventral neural regions of the presumptive spinal cord except for in the floor plate

• in the dorsal neural tube of E12.5 embryos, endothelial cells and pericytes form abnormal clusters and enlarged lumens in the vascular structures present

intracranial hemorrhage ( J:142352 )

• hemorrhaging is detected throughout the developing brain of E12.5 embryos

spinal hemorrhage ( J:142352 )

• hemorrhaging is detected throughout the developing spine of E12.5 embryos

abnormal blood-brain barrier function ( J:142352 )

• hemorrhaging and downregulation of the endothelial marker GLUT-1 suggest a defect in the blood brain barrier of developing embryos

nervous system

intracranial hemorrhage ( J:142352 )

• hemorrhaging is detected throughout the developing brain of E12.5 embryos

spinal hemorrhage ( J:142352 )

• hemorrhaging is detected throughout the developing spine of E12.5 embryos

abnormal blood-brain barrier function ( J:142352 )

• hemorrhaging and downregulation of the endothelial marker GLUT-1 suggest a defect in the blood brain barrier of developing embryos

Genotype
MGI:3831188

cn3

• Allelic Composition: Tg(Nes-cre)1Kln/0; Wnt7a^tm1Amc/Wnt7a^tm1Amc; Wnt7b^tm1Parr/Wnt7b^tm2Amc
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:142352 )

• all embryos die around E12.5 due to severe hemorrhaging within the central nervous system

cardiovascular system

abnormal vasculogenesis ( J:142352 )

• the number of endothelial cells and pericytes present in the intraneural vascular plexus of E12.5 embryos is greatly reduced

intracranial hemorrhage ( J:142352 )

• hemorrhaging is detected throughout the developing brain of E12.5 embryos

spinal hemorrhage ( J:142352 )

• hemorrhaging is detected throughout the developing spine of E12.5 embryos

abnormal blood-brain barrier function ( J:142352 )

• hemorrhaging and downregulation of the endothelial marker GLUT-1 suggest a defect in the blood brain barrier of developing embryos

nervous system

intracranial hemorrhage ( J:142352 )

• hemorrhaging is detected throughout the developing brain of E12.5 embryos

spinal hemorrhage ( J:142352 )

• hemorrhaging is detected throughout the developing spine of E12.5 embryos

abnormal blood-brain barrier function ( J:142352 )

• hemorrhaging and downregulation of the endothelial marker GLUT-1 suggest a defect in the blood brain barrier of developing embryos

Genotype
MGI:3829882

cn4

• Allelic Composition: Gt(ROSA)26Sor^tm1Sor/?; Wnt7b^tm2Amc/Wnt7b^tm2.1Amc; Tg(Hoxb7-cre)13Amc/0
• Genetic Background: involves: 129S4/SvJaeSor * 129X1/SvJ * C57BL/6

renal/urinary system

dilated kidney collecting duct ( J:142685 )

• at E15.5 the collecting ducts are dilated and branch points are less evident

Genotype
MGI:3829881

cn5

• Allelic Composition: Wnt7b^tm2Amc/Wnt7b^tm2.1Amc; Tg(Hoxb7-cre)13Amc/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6

mortality/aging

postnatal lethality, complete penetrance ( J:142685 )

• survive to P11 - P12

renal/urinary system

decreased urine osmolality ( J:142685 )

• at P10, urine osmolality is 56% that of controls

abnormal kidney morphology ( J:142685 )

• at P11 - P12 kidneys are grossly abnormal

absent kidney medulla ( J:142685 )

• absent at P10

kidney failure ( J:142685 )

• by P11 - P12 kidneys are physiologically incompetent

homeostasis/metabolism

decreased urine osmolality ( J:142685 )

• at P10, urine osmolality is 56% that of controls

Genotype
MGI:3793499

cn6

• Allelic Composition: Wnt7b^tm2Amc/Wnt7b^tm2.1Amc; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * CBA

mortality/aging

neonatal lethality, complete penetrance ( J:134483 )

• mice die within hours of birth

respiratory system

lung hemorrhage ( J:134483 )

abnormal lung development ( J:134483 )

• lung epithelial and mesenchyme tip cell progenitors exhibit decreased replication compared to in wild-type mice

• however, normal cell differentiation and patterning are preserved

decreased mesenchymal cell proliferation involved in lung development ( J:134483 )

• mesenchyme tip cell progenitors exhibit decreased replication compared to in wild-type mice

thin lung-associated mesenchyme ( J:134483 )

• the lung mesenchyme is thinner than in wild-type mice

abnormal right lung middle lobe morphology ( J:134483 )

• the medial lobe bronchus of the right lung emanates from the cephalic lobe bronchus, unlike in wild-type mice

• however, the correct number of lobes is formed

small lung ( J:134483 )

• at E12.5

decreased lung weight ( J:134483 )

• lung weight is 50% of wild-type at E18.5 and 25% of wild-type at E14.5

atelectasis ( J:134483 )

• lungs are poorly inflated

abnormal tracheal cartilage morphology ( J:134483 )

• cartilaginous rings are incomplete

homeostasis/metabolism

cyanosis ( J:134483 )

• after birth mice become cyanotic despite chest wall movements

renal/urinary system

increased kidney apoptosis ( J:142685 )

• at E17.5, a marked increase in the rate of apoptosis is seen in distal collecting duct epithelial cells

abnormal kidney cell proliferation ( J:142685 )

• at E15.5, the majority of cells in the collecting duct divide along the radial axis unlike in controls where the majority of cells divide along the longitudinal axis

decreased kidney cell proliferation ( J:142685 )

• cell proliferation in the loop of Henle is significantly reduced

abnormal kidney morphology ( J:142685 )

• renal corpuscles abut the renal pelvis

• despite the absence of the medulla, kidneys are similar in size to controls

abnormal kidney collecting duct epithelium morphology ( J:142685 )

• at E15.5, the majority of cells in the collecting duct divide along the radial axis unlike in controls where the majority of cells divide along the longitudinal axis

• at E17.5, a marked increase in the rate of apoptosis is seen in distal collecting duct epithelial cells

abnormal kidney medulla development ( J:142685 )

• at E15.5 and E16.5, the nascent medulla is absent indicating a failure to initiate medulla formation

absent kidney medulla ( J:142685 )

• absent at E18.5

truncated loop of Henle ( J:142685 )

• at E18.5 the loop of Henle is truncated resembling morphology at an earlier stage prior to loop elongation

• cell proliferation in the loop of Henle is significantly reduced

hydroureter ( J:142685 )

• at E18.5, less than a third of mice show hydroureter like swelling of the pelvic region

skeleton

abnormal tracheal cartilage morphology ( J:134483 )

• cartilaginous rings are incomplete

cardiovascular system

lung hemorrhage ( J:134483 )

cellular

increased kidney apoptosis ( J:142685 )

• at E17.5, a marked increase in the rate of apoptosis is seen in distal collecting duct epithelial cells

abnormal kidney cell proliferation ( J:142685 )

• at E15.5, the majority of cells in the collecting duct divide along the radial axis unlike in controls where the majority of cells divide along the longitudinal axis

decreased kidney cell proliferation ( J:142685 )

• cell proliferation in the loop of Henle is significantly reduced

decreased mesenchymal cell proliferation involved in lung development ( J:134483 )

• mesenchyme tip cell progenitors exhibit decreased replication compared to in wild-type mice