All Phenotypes Epas1<tm1Mcs> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Epas1^tm1Mcs
targeted mutation 1, M Celeste Simon
MGI:3526731

Summary

9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Egln1^tm2.1Fsl/Egln1^tm2.1Fsl Epas1^tm1Mcs/Epas1^tm1Mcs Gt(ROSA)26Sor^{tm9(cre/ESR1)Arte}/Gt(ROSA)26Sor⁺	B6.Cg-Gt(ROSA)26Sor^{tm9(cre/ESR1)Arte} Egln1^tm2.1Fsl Epas1^tm1Mcs	MGI:5525147
cn2	Epas1^tm1Mcs/Epas1^tm1Mcs Hif1a^tm3Rsjo/Hif1a^tm3Rsjo Vhl^tm1Jae/Vhl^tm1Jae Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * CD-1	MGI:5432005
cn3	Epas1^tm1Mcs/Epas1^tm1Mcs Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0	involves: 129S1/Sv * 129X1/SvJ * CD-1	MGI:5432004
cn4	Epas1^tm1Mcs/Epas1^tm1Mcs Hif1a^tm3Rsjo/Hif1a^tm3Rsjo Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0	involves: 129S1/Sv * 129X1/SvJ * CD-1	MGI:5432003
cn5	Epas1^tm1Mcs/Epas1^tm1Mcs Vhl^tm1Jae/Vhl^tm1Jae Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0	involves: 129S4/SvJae * 129X1/SvJ * CD-1	MGI:5432007
cn6	Egln1^tm1.1Brei/Egln1^tm1.1Brei Epas1^tm1Mcs/Epas1^tm1Mcs Tg(CD68-icre)1Bwlx/0	involves: 129X1/SvJ * C57BL/6	MGI:5568400
cn7	Epas1^tm1Mcs/Epas1^tm1Mcs Tg(CD68-icre)1Bwlx/0	involves: 129X1/SvJ * C57BL/6	MGI:5568403
cn8	Epas1^tm1Mcs/Epas1^tm1.1Mcs Tg(CAG-cre/Esr1*)5Amc/0	involves: 129X1/SvJ * C57BL/6 * CBA	MGI:6724166
cn9	Epas1^tm1Mcs/Epas1^tm1.1Mcs Ndor1^{Tg(UBC-cre/ERT2)1Ejb}/0	involves: 129X1/SvJ * FVB/N	MGI:3710346

Allelic Composition	Egln1^tm2.1Fsl/Egln1^tm2.1Fsl Epas1^tm1Mcs/Epas1^tm1Mcs Gt(ROSA)26Sor^{tm9(cre/ESR1)Arte}/Gt(ROSA)26Sor⁺
Genetic Background	B6.Cg-Gt(ROSA)26Sor^{tm9(cre/ESR1)Arte} Egln1^tm2.1Fsl Epas1^tm1Mcs

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Egln1^tm2.1Fsl mutation (0 available); any Egln1 mutation (22 available)
Epas1^tm1Mcs mutation (1 available); any Epas1 mutation (66 available)
Gt(ROSA)26Sor^{tm9(cre/ESR1)Arte} mutation (2 available); any Gt(ROSA)26Sor mutation (993 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:202737 )

N	• hematocrit level, erythropoietin level and spleen architecture and weight are similar to mice without cre/ESR1 allele • the Epas1 allele functions to rescue the erythrocytosis phenotype

mortality/aging

premature death ( J:202737 )

• survival is improved relative to mice carrying the Egln1 null allele, but not to control levels

Allelic Composition	Epas1^tm1Mcs/Epas1^tm1Mcs Hif1a^tm3Rsjo/Hif1a^tm3Rsjo Vhl^tm1Jae/Vhl^tm1Jae Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0
Genetic Background	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * CD-1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Epas1^tm1Mcs mutation (1 available); any Epas1 mutation (66 available)
Hif1a^tm3Rsjo mutation (3 available); any Hif1a mutation (50 available)
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc mutation (2 available)
Vhl^tm1Jae mutation (2 available); any Vhl mutation (18 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

skeleton

decreased trabecular bone volume ( J:186085 )

decreased bone trabecula number ( J:186085 )

hematopoietic system

hematopoietic system phenotype ( J:186085 )

N	• mice exhibit normal frequencies of KLS cells (hematopoietic stem cells and multipotential progenitors) numbers of red blood cells and lymphocytes and hematocrit

homeostasis/metabolism

decreased circulating erythropoietin level ( J:186085 )

Allelic Composition	Epas1^tm1Mcs/Epas1^tm1Mcs Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * CD-1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Epas1^tm1Mcs mutation (1 available); any Epas1 mutation (66 available)
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased erythroid progenitor cell number ( J:186085 )

• decreased frequency of CD71+/Ter119+ cells in the bone marrow

Allelic Composition	Epas1^tm1Mcs/Epas1^tm1Mcs Hif1a^tm3Rsjo/Hif1a^tm3Rsjo Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * CD-1

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:186085 )

N	• mice exhibit normal hematopoietic stem cell frequency

decreased erythroid progenitor cell number ( J:186085 )

• decreased frequency of CD71+/Ter119+ cells in the bone marrow

skeleton

decreased trabecular bone volume ( J:186085 )

Allelic Composition	Epas1^tm1Mcs/Epas1^tm1Mcs Vhl^tm1Jae/Vhl^tm1Jae Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0
Genetic Background	involves: 129S4/SvJae * 129X1/SvJ * CD-1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Epas1^tm1Mcs mutation (1 available); any Epas1 mutation (66 available)
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc mutation (2 available)
Vhl^tm1Jae mutation (2 available); any Vhl mutation (18 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:186085 )

N	• mice exhibit normal hematocrit

homeostasis/metabolism

decreased circulating erythropoietin level ( J:186085 )

Allelic Composition	Egln1^tm1.1Brei/Egln1^tm1.1Brei Epas1^tm1Mcs/Epas1^tm1Mcs Tg(CD68-icre)1Bwlx/0
Genetic Background	involves: 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Egln1^tm1.1Brei mutation (0 available); any Egln1 mutation (22 available)
Epas1^tm1Mcs mutation (1 available); any Epas1 mutation (66 available)
Tg(CD68-icre)1Bwlx mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:194598 )

N	• no premature death is observed

hematopoietic system

hematopoietic system phenotype ( J:194598 )

N	• erythropoiesis phenotype seen in Phd2 single mutants is absent from double mutants

decreased hematocrit ( J:194598 )

• HCT is lower than wild type

decreased hemoglobin content ( J:194598 )

• lower than in wild-type

Allelic Composition	Epas1^tm1Mcs/Epas1^tm1Mcs Tg(CD68-icre)1Bwlx/0
Genetic Background	involves: 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Epas1^tm1Mcs mutation (1 available); any Epas1 mutation (66 available)
Tg(CD68-icre)1Bwlx mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:194598 )

N	• no premature death observed

Allelic Composition	Epas1^tm1Mcs/Epas1^tm1.1Mcs Tg(CAG-cre/Esr1*)5Amc/0
Genetic Background	involves: 129X1/SvJ * C57BL/6 * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Epas1^tm1.1Mcs mutation (0 available); any Epas1 mutation (66 available)
Epas1^tm1Mcs mutation (1 available); any Epas1 mutation (66 available)
Tg(CAG-cre/Esr1*)5Amc mutation (10 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cardiovascular system

cardiovascular system phenotype ( J:302384 )

N	• tamoxifen-treated mice exhibit normal carotid body morphology

heart left ventricle hypertrophy ( J:302384 )

• likely secondary to anemia in tamoxifen-treated

abnormal type I cell of carotid body physiology ( J:302384 )

• impaired responsiveness to hypoxia with reduced secretory rate in tamoxifen-treated mice

• lower shifted quantal charge and reduced mean charge per event in tamoxifen-treated mice exposed to mild stimuli

• reduced increase in NADH and mitochondrial function induced by hypoxia in tamoxifen-treated mice

• however, tamoxifen-treated mice exhibit normal hypercapnia and depolarization with high extracellular potassium ion, and normal secretion induced by CO2 and high potassium ion

hematopoietic system

anemia ( J:302384 )

• in tamoxifen-treated mice

decreased hematocrit ( J:302384 )

• in tamoxifen-treated mice

homeostasis/metabolism

abnormal physiological response to hypoxia ( J:302384 )

• decreased hypoxic ventilatory response in tamoxifen-treated mice exposed to hypoxic conditions

• however, response to hypercapnia is normal and tamoxifen-treated mice exhibit a small transient hyperventilatory response at the onset of exposure to hypoxia

growth/size/body

heart left ventricle hypertrophy ( J:302384 )

• likely secondary to anemia in tamoxifen-treated

muscle

heart left ventricle hypertrophy ( J:302384 )

• likely secondary to anemia in tamoxifen-treated

nervous system

abnormal type I cell of carotid body physiology ( J:302384 )

• impaired responsiveness to hypoxia with reduced secretory rate in tamoxifen-treated mice

• lower shifted quantal charge and reduced mean charge per event in tamoxifen-treated mice exposed to mild stimuli

• reduced increase in NADH and mitochondrial function induced by hypoxia in tamoxifen-treated mice

• however, tamoxifen-treated mice exhibit normal hypercapnia and depolarization with high extracellular potassium ion, and normal secretion induced by CO2 and high potassium ion

Allelic Composition	Epas1^tm1Mcs/Epas1^tm1.1Mcs Ndor1^{Tg(UBC-cre/ERT2)1Ejb}/0
Genetic Background	involves: 129X1/SvJ * FVB/N

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

enlarged spleen ( J:119731 )

• spleens are enlarged relative to controls (0.5% spleen weight to body weight vs. ~0.4% in controls)

anemia ( J:119731 )

• occurs after postnatal cre induction

abnormal proerythroblast morphology ( J:119731 )

• erythroid progenitors from bone marrow form ~50% fewer erythroid burst-forming units (BFU-E) and colony-forming units (CFU-E) in culture than control cells, whereas erythroid progenitors from the spleen form more BFU-E and CFU-E

• there are fewer CD71+ immature erythroid progenitors in the bone marrow, and a higher percentage of CD71+ /Ter119+ double positive cells in the spleen

decreased erythrocyte cell number ( J:119731 )

• with acute deletion of Epas1 at 6-8 weeks induced by tamoxifen administration, animals have decreased red blood cell numbers relative to controls

decreased hematocrit ( J:119731 )

• with acute deletion of Epas1 at 6-8 weeks induced by tamoxifen administration, animals have decreased hematocrit relative to controls

decreased hemoglobin content ( J:119731 )

• with acute deletion of Epas1 at 6-8 weeks induced by tamoxifen administration, animals have decreased hemoglobin levels relative to controls

reticulocytopenia ( J:119731 )

• numbers are decreased compared to controls

abnormal hematopoietic system physiology ( J:119731 )

• mice show weak induction of erythropoietin after phenylhydrazine treatment

immune system

enlarged spleen ( J:119731 )

• spleens are enlarged relative to controls (0.5% spleen weight to body weight vs. ~0.4% in controls)

growth/size/body

enlarged spleen ( J:119731 )

• spleens are enlarged relative to controls (0.5% spleen weight to body weight vs. ~0.4% in controls)

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