Phenotypes associated with this allele

• Allele Symbol: Matk^tm1Sor
• Allele Name: targeted mutation 1, Philippe Soriano
• Allele ID: MGI:3526800
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Matk^tm1Sor/Matk^tm1Sor	B6.129S4-Matk^tm1Sor	MGI:3626226

Genotype
MGI:3626226

hm1

• Allelic Composition: Matk^tm1Sor/Matk^tm1Sor
• Genetic Background: B6.129S4-Matk^tm1Sor

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:109203 )

N • normal populations of mononuclear cells expressing the B cell lineage surface markers CD19 and B220/CD45R

N • normal populations of double negative (DN: CD4^- CD8^-), single positive (SP: CD4⁺ CD8^- and CD4^- CD8⁺) and double positive (DP: CD4⁺ CD8⁺) T cells

N • normal populations of monocytic (CD11b⁺ GR^-) and of granulocytic (CD11b⁺ GR⁺ cells

N • normal frequencies of common myeloid progenitors (CMP) and of common lymphoid progenitors (CLP) in bone marrow

N • total bone marrow cells of mutant and of wild-type mice cultured for 10-14 days in a CFU-mix cytokine cocktail yield similar numbers of CFU-mix colonies

N • Th1 and Th2 T cells differentiated in vitro from wild-type and mutant CD4⁺ T cells secrete similar amounts of Th1 (IL-2, IFN-gamma) and Th2 (IL-4, IL-10) cytokines, respectively

N • similar proliferation is observed in lymph node (LN) cells from mutant and from wild-type mice immunized with TNP-ovalbumin/incomplete Freund's adjuvant (TNP-OVA/IFA) and restimulated in vitro

N • LN cells from mutant mice immunized with TNP-OVA/IFA and restimulated in vitro secrete IL-10 and IL-4 at nearly identical, low levels, as do wild-type cells

N • in vitro restimulated spleen cells from TNP-OVA/IFA immunized mutant and wild-type mice secrete similar amounts of IL-12

N • IL-12 levels are similar in sera and in lymph nodes of TNP-OVA/IFA immunized mutant and wild-type mice

abnormal bone marrow cell morphology/development ( J:109203 )

• total bone marrow cells of mutant mice cultured for 10-12 days in the presence of IL-7 yield ~4-fold as many pre-B cell colonies as did BM cells from wild-type mice

increased hematopoietic stem cell number ( J:109203 )

• homozygous mutant mice have approximately 2.2-fold as many SPKLS (side population KIT⁺ LIN^- LY6A/SCA-1⁺) cells, a primitive hematopoietic stem cell population, as do wild-type mice

abnormal hematopoietic system physiology ( J:109203 )

• LN cells from mutant mice stimulated with TNP-OVA/IFA secrete slightly more IL-2 than do wild-type cells

• LN and spleen cells from mutant mice stimulated with TNP-OVA/IFA secrete significantly less IFN-gamma than do wild-type cells