Analysis Tools|
Allele Symbol Allele Name Allele ID |
Gad1tm1Rpa targeted mutation 1, Richard D Palmiter MGI:3527168 |
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| Summary |
5 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• following transfection with a cre-recombinase vector, the axons of basket interneurons are thinner and have reduced numbers of finer branches especially terminal branches compared to in control cells
• following transfection with a cre-recombinase vector, terminal branches have fewer and smaller boutons around pyramidal cell somata throughout the innervation field (2.7+/-0.4 boutons per soma compared to 8.9+/-0.7 boutons per soma in control cells)
• following transfection with a cre-recombinase vector, it is difficult to identify synaptic vesicles and synaptic specialization unlike in control interneurons
• following transfection with a cre-recombinase vector, basket interneurons display reduced axon density and percentage of targeted neuronal somatafollowing transfection with a cre-recombinase vector, basket interneurons display reduced axon density and percentage of targeted neuronal somata
• however, following transfection with a cre-recombinase vector, the shape and gross branching patterns are normal
• however, baclofen treatment following transfection with a cre-recombinase vector partially resuces bouton density (6.1+/-0.5 boutons per soma compared to 2.7+/-0.4 in untreated cells)
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• following infection with an adenovirus-cre, basket interneurons in the visual cortex have thinner axon terminals, frequent stretches without boutons around pyramidal cell somata, and smaller perisomatic bouton size (40% reduction in bouton diameter)
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• following infection with an andenovirus-cre, the axons of basket interneurons are thinner and have reduced numbers of finer branches especially terminal branches compared to in control cells
• following infection with an andenovirus-cre, terminal branches have fewer and smaller boutons around pyramidal cell somata throughout the innervation field (5.0+/-0.3 boutons per soma compared to 8.9+/-0.4 boutons per soma in control cells)
• however, NO711 treatment following infection with an andenovirus-cre rescues perisomatic bouton formation and increases complexity of ternimal branches, bouton density, and size of perisomatic boutons while treatment with diazepam treated cells show much less pronounced perisomatic synapse defects
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice exhibit normal gait and response to the Drd1a agonist SKF81297
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• mice spend more time on a rotarod than heterozygous controls
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• on the second of three trials mice exhibit decreased strength in a modified wire hang test compared to heterozygous controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at E17.5 in 9 of 11 mice
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• umbilical hernia is seen at E17.5 in 6 of 11 mice
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• at E17.5 in 9 of 11 mice
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• at E17.5 in 9 of 11 mice
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/10/2024 MGI 6.24 |
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