Analysis Tools|
Allele Symbol Allele Name Allele ID |
Agrptm2(HBEGF)Rpa targeted mutation 2, Richard D Palmiter MGI:3527177 |
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| Summary |
9 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• following treatment with diptheria toxin (DT) to specifically ablate NPY-expressing cells, mice fail to increase their food intake at a 10% loss of body weight and their intake dramatically decreased as their weight loss approaches 20%
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• adult mice injected intramuscularly with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit a greater than 80% drop in feeding, a 20% reduction in their body weight and have to be euthanized unlike similarly treated wild-type mice
• however, most mice survive when injected with DT as neonate or when receiving injections as neonate and adults
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• adult mice injected intramuscularly with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit increased water consumption compared to similarly treated wild-type mice
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• adult mice injected intramuscularly with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit a greater than 80% drop in feeding unlike similarly treated wild-type mice
• adults injected with DT cease to eat unlike similarly treated wild-type mice
• however, DT-treated mice are sustained by hand feeding via oral gavage with liquid food
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• adult mice injected intramuscularly with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit 20% of their body weight unlike similarly treated wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• adults injected with diptheria toxin (DT) to specifically ablate NPY-expressing cells cease to eat unlike similarly treated wild-type mice
• however, food consumption of neonates following injection with diptheria toxin to specifically ablate NPY-expressing cells is normal
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• neonates injection with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit an 11% decreased in body weight at weaning compared to similarly treated wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• diphtheria-treated mice bilaterally injected with a virus expressing a AAV1-CreGFP into the parabrachial nucleus
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• diphtheria-treated mice injected with a virus expressing a AAV1-CreGFP into the nucleus tractus solitaries or parabrachial nucleus prevents starvation induced by AgRP neuron ablation unlike in control mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in diphtheria-treated mice injected with a virus expressing a CAV2-cre into the nucleus tractus solitaries
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• diphtheria-treated mice injected with a virus expressing a CAV2-cre into the nucleus tractus solitaries prevents starvation induced by AgRP neuron ablation unlike in control mice
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• in diphtheria-treated mice injected with a virus expressing a CAV2-cre into the nucleus tractus solitaries
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• diphtheria-treated mice injected with a virus expressing a AAV1-CreGFP into the nucleus tractus solitaries or parabrachial nucleus prevents starvation induced by AgRP neuron ablation unlike in control mice
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• mice injected with a virus expressing a AAV1-CreGFP into the parabrachial nucleus without ablation of AgRP neurons
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• mice injected with a virus expressing a AAV1-CreGFP into the parabrachial nucleus whether treated with diphtheria or not
• in diphtheria treated mice bilaterally with a virus expressing a AAV1-CreGFP into the nucleus tractus solitaries
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• young adult mice stop eating and would succumb following treatment with diptheria toxin
• older moderately obese (35-40 g) mice survive diptheria toxin treatment despite weight loss
• however, mice that weigh over 40 g succumb following treatment with diptheria toxin
• norepinephrine treatment prevents prolongs the survival of diptheria toxin treated mice that weigh over 40 g
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• loss of agouti-related protein expressing neurons following treatment with diptheria toxin
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• following treatment with diptheria toxin young adult mice gradually stop eating by day 6 post treatment
• older, moderately obese mice that are able to survive the weight loss resume feeding about 18 days after treatment
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• diptheria toxin treated older moderately obese mice weigh less than saline treated controls for several months after treatment
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• following treatment with diptheria toxin mice lose 20% of their body weight
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• diptheria toxin treatment of mice that weigh over 40 g results in a sharp drop in body temperature to near ambient temperature as mice become moribund
• norepinephrine treatment prevents the drop in body temperature
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• glucose tolerance is improved in diptheria toxin treated mice relative to saline treated controls and is not significantly different from mice heterozygous for Lepob alone
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• following diptheria toxin treatment of older moderately obese mice, both males and females become fertile, unlike saline treated mice which remain infertile
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• adult mice treated with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit 20% of their body weight unlike similarly treated wild-type mice
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• mice treated with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit miniature inhibitory postsynaptic currents that are 6-fold lower than in wild-type mice and lower than in fasted wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in mice treated with diptheria toxin (DT) to specifically ablate NPY-expressing cells
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• in mice treated with diptheria toxin (DT) to specifically ablate NPY-expressing cells
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/17/2024 MGI 6.24 |
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