Phenotypes associated with this allele

• Allele Symbol: Calca^tm1Rfg
• Allele Name: targeted mutation 1, Robert F Gagel
• Allele ID: MGI:3527257
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Calca^tm1Rfg/Calca^tm1Rfg	involves: 129S7/SvEvBrd * C57BL/6	MGI:3527477
hm2	Calca^tm1Rfg/Calca^tm1Rfg	involves: Black Swiss	MGI:3527478

Genotype
MGI:3527477

hm1

• Allelic Composition: Calca^tm1Rfg/Calca^tm1Rfg
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

abnormal circulating calcium level ( J:80827 )

• treatment with human pituitary hormone significantly increased serum calcium in mutants but not in wild-type mice

abnormal urine homeostasis ( J:80827 )

• treatment with human pituitary hormone significantly increased levels of deoxypyridinoline in the urine in mutants but not in wild-type mice

renal/urinary system

abnormal urine homeostasis ( J:80827 )

• treatment with human pituitary hormone significantly increased levels of deoxypyridinoline in the urine in mutants but not in wild-type mice

skeleton

abnormal osteoblast physiology ( J:80827 )

• osteoblast function is increased with a 1.5- and 2-fold increase in bone formation at 1 and 3 months of age, respectively

• however, osteoclast function is normal

increased bone mineral density ( J:80827 )

• bone density is increased in 1 and 3 month old female mutants and in 3 month old male mutants compared to wild-type mice

abnormal trabecular bone morphology ( J:80827 )

• trabecular bone volume is increased in the vertebral bodies at 1 and 3 months and in the tibia at 3 months in female mutants

• trabecular thickness is the same however trabecular spacing is decreased in male and female mutants at 3 months of age

• after ovariectomy trabecular bone volume does not decrease in mutants unlike in wild-type mice

decreased bone strength ( J:80827 )

• after ovariectomy bone strength is decreased by 10% in mutants compared to a 35% decrease in wild-type mice

cellular

abnormal osteoblast physiology ( J:80827 )

• osteoblast function is increased with a 1.5- and 2-fold increase in bone formation at 1 and 3 months of age, respectively

• however, osteoclast function is normal

Genotype
MGI:3527478

hm2

• Allelic Composition: Calca^tm1Rfg/Calca^tm1Rfg
• Genetic Background: involves: Black Swiss

embryo

embryo phenotype ( J:80827 )

N • placental weight and structure are normal

homeostasis/metabolism

abnormal circulating magnesium level ( J:80827 )

• during pregnancy maternal magnesium levels are decreased however calcium levels are similar to wild-type

• serum magnesium levels, but not calcium levels, are also decreased in null fetuses when the mother is either heterozygous or homozygous null

skeleton

abnormal bone mineralization ( J:80827 )

• magnesium content of homozygous fetuses from homozygous mothers is significantly decreased compared to heterozygous fetuses, however magnesium content is not significantly decreased in homozygous fetuses from heterozygous mothers