Phenotypes associated with this allele

• Allele Symbol: Tg(OPN1LW-cre)4Yzl
• Allele Name: transgene insertion 4, Yun-Zheng Le
• Allele ID: MGI:3527258
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pik3ca^tm1Jjz/Pik3ca^tm1Jjz Tg(OPN1LW-cre)4Yzl/0	involves: 129 * FVB/N	MGI:6382534
cn2	Nampt^tm1Oleo/Nampt^tm1Oleo Tg(OPN1LW-cre)4Yzl/0	involves: 129P2/OlaHsd * FVB/N	MGI:6383810
cn3	Pik3r1^tm1Lca/Pik3r1^tm1Lca Tg(OPN1LW-cre)4Yzl/0	involves: 129S6/SvEvTac * FVB/N	MGI:6382536
cn4	Ift20^tm1.1Gjp/Ift20^tm1.2Gjp Tg(OPN1LW-cre)4Yzl/0	involves: 129S7/SvEvBrd * C57BL/6J * FVB/N	MGI:6383662
cn5	Tmem30a^tm1.1Xjz/Tmem30a^tm1.1Xjz Tg(OPN1LW-cre)4Yzl/0	involves: 129/SvEv * C57BL/6J * FVB/N	MGI:6382628
cn6	Emc1^em1Xjz/Emc1^em1Xjz Tg(OPN1LW-cre)4Yzl/0	involves: C57BL/6J * FVB/N	MGI:7611971
cn7	Elovl4^tm3Kzh/Elovl4^tm3Kzh Tg(OPN1LW-cre)4Yzl/?	involves: FVB/N	MGI:5492072

Genotype
MGI:6382534

cn1

• Allelic Composition: Pik3ca^tm1Jjz/Pik3ca^tm1Jjz; Tg(OPN1LW-cre)4Yzl/0
• Genetic Background: involves: 129 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

vision/eye

retina cone cell degeneration ( J:282245 )

• cone cell loss in 3 month old mice

• however, overall morphology and structural integrity of retinas is normal at 3 months of age

decreased b-wave amplitude ( J:282245 )

• photopic ERG b-wave recordings of cone photoreceptors are lower at 3 months of age

• however, scotopic ERG recordings of rod photoreceptors are normal at 3 months of age

nervous system

retina cone cell degeneration ( J:282245 )

• cone cell loss in 3 month old mice

• however, overall morphology and structural integrity of retinas is normal at 3 months of age

Genotype
MGI:6383810

cn2

• Allelic Composition: Nampt^tm1Oleo/Nampt^tm1Oleo; Tg(OPN1LW-cre)4Yzl/0
• Genetic Background: involves: 129P2/OlaHsd * FVB/N

vision/eye

retina cone cell degeneration ( J:265592 )

• at 3 weeks with massive atrophy of the neurosensory retina and vascular attenuation with pigment mottling

retina pigment epithelium atrophy ( J:265592 )

• at 3 weeks

abnormal cone electrophysiology ( J:265592 )

• photopic b-wave amplitude

• reduced visual acuity

• however, nicotinamide mononucleotide treatment rescued function

abnormal rod electrophysiology ( J:265592 )

• mild decline inn scotopic a- and b-wave amplitudes

• however, nicotinamide mononucleotide treatment rescued function

nervous system

retina cone cell degeneration ( J:265592 )

• at 3 weeks with massive atrophy of the neurosensory retina and vascular attenuation with pigment mottling

pigmentation

retina pigment epithelium atrophy ( J:265592 )

• at 3 weeks

Genotype
MGI:6382536

cn3

• Allelic Composition: Pik3r1^tm1Lca/Pik3r1^tm1Lca; Tg(OPN1LW-cre)4Yzl/0
• Genetic Background: involves: 129S6/SvEvTac * FVB/N

vision/eye

abnormal retina cone cell morphology ( J:232389 )

• progressive disorganization of synaptic ultrastructure is seen in the terminals of surviving cones at 6 and 12 months of age but not at 1 month

retina cone cell degeneration ( J:232389 )

• retinas show patchy regions devoid of cones by 6 months and cone photoreceptor loss is further exacerbated by 12 months of age

• the inferior meridian region of the retinas shows greater cone loss than the superior region at 6 months but by 12 months, similar loss of cones is seen in both regions

• however, the overall morphology and structural integrity of the retina are similar to wild-type mice at 1, 6, and 12 months of age

decreased b-wave amplitude ( J:232389 )

• scotopic b-wave amplitudes are lower at 6 and 12 months of age compared with control floxed mice but are not different from control cre-only expressing mice

• photopic ERG b-wave amplitudes are decreased at 6 months, but not 1 month of age, and decrease progressively to 12 months

• however, scotopic ERG recordings of rod photoreceptors (scotopic a-wave) are normal at 1, 6, and 12 months of age

nervous system

abnormal retina cone cell morphology ( J:232389 )

• progressive disorganization of synaptic ultrastructure is seen in the terminals of surviving cones at 6 and 12 months of age but not at 1 month

retina cone cell degeneration ( J:232389 )

• retinas show patchy regions devoid of cones by 6 months and cone photoreceptor loss is further exacerbated by 12 months of age

• the inferior meridian region of the retinas shows greater cone loss than the superior region at 6 months but by 12 months, similar loss of cones is seen in both regions

• however, the overall morphology and structural integrity of the retina are similar to wild-type mice at 1, 6, and 12 months of age

abnormal synapse morphology ( J:232389 )

• progressive disorganization of synaptic ultrastructure is seen in the terminals of surviving cones at 6 and 12 months of age but not at 1 month

abnormal ribbon synapse morphology ( J:232389 )

• cone terminals in the retina ribbon synaptic complexes are reduced, flat contacts are sometimes located adjacent to synaptic ribbons, and interactions with postsynaptic processes appear disorganized

Genotype
MGI:6383662

cn4

• Allelic Composition: Ift20^tm1.1Gjp/Ift20^tm1.2Gjp; Tg(OPN1LW-cre)4Yzl/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J * FVB/N

nervous system

abnormal retina cone cell inner segment morphology ( J:183002 )

• mice show mislocalization of opsin in the inner segment and at the synapse such that opsin is abundant in the inner segments at P10, with dense accumulations at the basal body of the connecting cilium, suggesting a defect in cone opsin active transport through the cilium

abnormal retina cone cell outer segment morphology ( J:183002 )

• cone outer segments show reduced amounts of opsin at P10

photoreceptor outer segment degeneration ( J:183002 )

• the number of outer segments declines rapidly after P10 such that only about 10% as many as in controls are seen at P28 and none are seen at later time points

retina cone cell degeneration ( J:183002 )

• rapid degeneration after P10

vision/eye

abnormal retina cone cell inner segment morphology ( J:183002 )

• mice show mislocalization of opsin in the inner segment and at the synapse such that opsin is abundant in the inner segments at P10, with dense accumulations at the basal body of the connecting cilium, suggesting a defect in cone opsin active transport through the cilium

abnormal retina cone cell outer segment morphology ( J:183002 )

• cone outer segments show reduced amounts of opsin at P10

photoreceptor outer segment degeneration ( J:183002 )

• the number of outer segments declines rapidly after P10 such that only about 10% as many as in controls are seen at P28 and none are seen at later time points

retina cone cell degeneration ( J:183002 )

• rapid degeneration after P10

Genotype
MGI:6382628

cn5

• Allelic Composition: Tmem30a^tm1.1Xjz/Tmem30a^tm1.1Xjz; Tg(OPN1LW-cre)4Yzl/0
• Genetic Background: involves: 129/SvEv * C57BL/6J * FVB/N

nervous system

abnormal retina cone cell morphology ( J:253580 )

• significant reduction in M-opsin expression

• mislocalization of M-opsin to inner segment (IS) and cell bodies at age P16

absent retina cone cells ( J:253580 )

• normal cone cell number at age P16

• no cone cells in outer segment at age P42

retina cone cell degeneration ( J:253580 )

• normal cone cell number at age P16

• no cone cells in outer segment at age P42

vision/eye

abnormal retina cone cell morphology ( J:253580 )

• significant reduction in M-opsin expression

• mislocalization of M-opsin to inner segment (IS) and cell bodies at age P16

absent retina cone cells ( J:253580 )

• normal cone cell number at age P16

• no cone cells in outer segment at age P42

retina cone cell degeneration ( J:253580 )

• normal cone cell number at age P16

• no cone cells in outer segment at age P42

abnormal cone electrophysiology ( J:253580 )

• no a- or b-wave with photopic ERG

Genotype
MGI:7611971

cn6

• Allelic Composition: Emc1^em1Xjz/Emc1^em1Xjz; Tg(OPN1LW-cre)4Yzl/0
• Genetic Background: involves: C57BL/6J * FVB/N

vision/eye

abnormal retina cone cell morphology ( J:345827 )

• at P25 cones appear curled with disordered arrangement

decreased retina cone cell number ( J:345827 )

• only a small residual number of cones are present at 2 months of age

retina cone cell degeneration ( J:345827 )

nervous system

abnormal retina cone cell morphology ( J:345827 )

• at P25 cones appear curled with disordered arrangement

decreased retina cone cell number ( J:345827 )

• only a small residual number of cones are present at 2 months of age

retina cone cell degeneration ( J:345827 )

Genotype
MGI:5492072

cn7

• Allelic Composition: Elovl4^tm3Kzh/Elovl4^tm3Kzh; Tg(OPN1LW-cre)4Yzl/?
• Genetic Background: involves: FVB/N

vision/eye

vision/eye phenotype ( J:194246 )

N • normal ERG and normal visual performance up to 6.5 months

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:194246 )

N • very long chain polyunsaturated fatty acid content of rods not abnormally increased