About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Hrh4tm1Lex
targeted mutation 1, Lexicon Genetics
MGI:3528886
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Hrh4tm1Lex/Hrh4tm1Lex involves: 129S/SvEv * BALB/c MGI:3775273
hm2
 		Hrh4tm1Lex/Hrh4tm1Lex involves: 129S/SvEv * C57BL/6 MGI:3775270
ht3
 		Hrh4tm1Lex/Hrh4+ involves: 129S/SvEv * C57BL/6 MGI:3775271


Genotype
MGI:3775273
hm1
Allelic
Composition		 Hrh4tm1Lex/Hrh4tm1Lex
Genetic
Background		 involves: 129S/SvEv * BALB/c
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hrh4tm1Lex mutation (1 available); any Hrh4 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased IgE level ( J:131785 )
• ovalbumin-specific IgE is decreased 54% compared to in wild-type mice
decreased IgG1 level ( J:131785 )
• following exposure to ovalbumin, IgG1 levels are decreased 18% compared to in wild-type mice
• however, IgG2a levels are normal
abnormal chemokine level ( J:131785 )
• LPS, CpG or Pam3Cys stimulation results in 47% less Cxcl1 and 36% less Ccl3 than wild-type mice
• CpG stimulation results in decreased Cxcl10 compared to in wild-type mice
abnormal interleukin level ( J:131785 )
• dendritic cells produce less IL-6 in response to LPS stimulation compared to wild-type cells
• restimulation of splenocytes with ovalbumin results in decreased levels of IL-4 (69%), IL-5 (28%), and IL-17 (67%) compared to wild-type mice
• cultured peribronchiolar lymph nodes stimulated with ovalbumin produce 86% less IL-4, 95% less IL-5, 67% less IL-13, 58% less IL-6, and 92% less IL-17 compared to wild-type mice
abnormal dendritic cell physiology ( J:131785 )
• while dendritic cells are capable of promoting proliferation of T cells isolated from Tg(DO11.10)10Dlo expressing mice, these T cells produce reduced IL-6 and IL-7 but not IL-5 when stimulated with anti-CD3 and anti-CD28 antibodies compared to wild-type dendritic cells
• dendritic cells produce less IL-6 in response to LPS, CpG or Pam3Cys stimulation compared to wild-type cells
decreased inflammatory response ( J:131785 )
• following exposure to ovalbumin-aerosol, mice exhibit a 56% decreased total cells in the branchoaveolar lavage including decreases in macrophages (37%), eosinophils (67%) and lymphocytes (75%) compared to in wild-type mice

homeostasis/metabolism
abnormal chemokine level ( J:131785 )
• LPS, CpG or Pam3Cys stimulation results in 47% less Cxcl1 and 36% less Ccl3 than wild-type mice
• CpG stimulation results in decreased Cxcl10 compared to in wild-type mice
abnormal interleukin level ( J:131785 )
• cultured peribronchiolar lymph nodes stimulated with ovalbumin produce 86% less IL-4, 95% less IL-5, 67% less IL-13, 58% less IL-6, and 92% less IL-17 compared to wild-type mice
• restimulation of splenocytes with ovalbumin results in decreased levels of IL-4 (69%), IL-5 (28%), and IL-17 (67%) compared to wild-type mice
• dendritic cells produce less IL-6 in response to LPS stimulation compared to wild-type cells

hematopoietic system
decreased IgE level ( J:131785 )
• ovalbumin-specific IgE is decreased 54% compared to in wild-type mice
decreased IgG1 level ( J:131785 )
• following exposure to ovalbumin, IgG1 levels are decreased 18% compared to in wild-type mice
• however, IgG2a levels are normal




Genotype
MGI:3775270
hm2
Allelic
Composition		 Hrh4tm1Lex/Hrh4tm1Lex
Genetic
Background		 involves: 129S/SvEv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hrh4tm1Lex mutation (1 available); any Hrh4 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal mast cell physiology ( J:131785 )
• unlike wild-type cells, mast cells fail to respond to 30 uM histamine stimulation by mobilizing calcium
• mast cells fail to migrate in response to histamine stimulation unlike wild-type mast cells

hematopoietic system
abnormal mast cell physiology ( J:131785 )
• unlike wild-type cells, mast cells fail to respond to 30 uM histamine stimulation by mobilizing calcium
• mast cells fail to migrate in response to histamine stimulation unlike wild-type mast cells




Genotype
MGI:3775271
ht3
Allelic
Composition		 Hrh4tm1Lex/Hrh4+
Genetic
Background		 involves: 129S/SvEv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hrh4tm1Lex mutation (1 available); any Hrh4 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal mast cell physiology ( J:132140 )
• mast cells do not respond to 30 uM histamine stimulation by mobilizing calcium as strongly as wild-type cells

hematopoietic system
abnormal mast cell physiology ( J:132140 )
• mast cells do not respond to 30 uM histamine stimulation by mobilizing calcium as strongly as wild-type cells




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
11/05/2024
MGI 6.24 		The Jackson Laboratory