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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Hrh4tm1Lex
targeted mutation 1, Lexicon Genetics
MGI:3528886
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Hrh4tm1Lex/Hrh4tm1Lex involves: 129S/SvEv * BALB/c MGI:3775273
hm2
 		Hrh4tm1Lex/Hrh4tm1Lex involves: 129S/SvEv * C57BL/6 MGI:3775270
ht3
 		Hrh4tm1Lex/Hrh4+ involves: 129S/SvEv * C57BL/6 MGI:3775271


Genotype
MGI:3775273
hm1
Allelic
Composition		 Hrh4tm1Lex/Hrh4tm1Lex
Genetic
Background		 involves: 129S/SvEv * BALB/c
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hrh4tm1Lex mutation (1 available); any Hrh4 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased IgE level ( J:131785 )
• ovalbumin-specific IgE is decreased 54% compared to in wild-type mice
decreased IgG1 level ( J:131785 )
• following exposure to ovalbumin, IgG1 levels are decreased 18% compared to in wild-type mice
• however, IgG2a levels are normal
abnormal chemokine level ( J:131785 )
• LPS, CpG or Pam3Cys stimulation results in 47% less Cxcl1 and 36% less Ccl3 than wild-type mice
• CpG stimulation results in decreased Cxcl10 compared to in wild-type mice
abnormal interleukin level ( J:131785 )
• cultured peribronchiolar lymph nodes stimulated with ovalbumin produce 86% less IL-4, 95% less IL-5, 67% less IL-13, 58% less IL-6, and 92% less IL-17 compared to wild-type mice
• restimulation of splenocytes with ovalbumin results in decreased levels of IL-4 (69%), IL-5 (28%), and IL-17 (67%) compared to wild-type mice
• dendritic cells produce less IL-6 in response to LPS stimulation compared to wild-type cells
abnormal dendritic cell physiology ( J:131785 )
• while dendritic cells are capable of promoting proliferation of T cells isolated from Tg(DO11.10)10Dlo expressing mice, these T cells produce reduced IL-6 and IL-7 but not IL-5 when stimulated with anti-CD3 and anti-CD28 antibodies compared to wild-type dendritic cells
• dendritic cells produce less IL-6 in response to LPS, CpG or Pam3Cys stimulation compared to wild-type cells
decreased inflammatory response ( J:131785 )
• following exposure to ovalbumin-aerosol, mice exhibit a 56% decreased total cells in the branchoaveolar lavage including decreases in macrophages (37%), eosinophils (67%) and lymphocytes (75%) compared to in wild-type mice

homeostasis/metabolism
abnormal chemokine level ( J:131785 )
• LPS, CpG or Pam3Cys stimulation results in 47% less Cxcl1 and 36% less Ccl3 than wild-type mice
• CpG stimulation results in decreased Cxcl10 compared to in wild-type mice
abnormal interleukin level ( J:131785 )
• cultured peribronchiolar lymph nodes stimulated with ovalbumin produce 86% less IL-4, 95% less IL-5, 67% less IL-13, 58% less IL-6, and 92% less IL-17 compared to wild-type mice
• restimulation of splenocytes with ovalbumin results in decreased levels of IL-4 (69%), IL-5 (28%), and IL-17 (67%) compared to wild-type mice
• dendritic cells produce less IL-6 in response to LPS stimulation compared to wild-type cells

hematopoietic system
decreased IgE level ( J:131785 )
• ovalbumin-specific IgE is decreased 54% compared to in wild-type mice
decreased IgG1 level ( J:131785 )
• following exposure to ovalbumin, IgG1 levels are decreased 18% compared to in wild-type mice
• however, IgG2a levels are normal




Genotype
MGI:3775270
hm2
Allelic
Composition		 Hrh4tm1Lex/Hrh4tm1Lex
Genetic
Background		 involves: 129S/SvEv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hrh4tm1Lex mutation (1 available); any Hrh4 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal mast cell physiology ( J:131785 )
• mast cells fail to migrate in response to histamine stimulation unlike wild-type mast cells
• unlike wild-type cells, mast cells fail to respond to 30 uM histamine stimulation by mobilizing calcium

hematopoietic system
abnormal mast cell physiology ( J:131785 )
• unlike wild-type cells, mast cells fail to respond to 30 uM histamine stimulation by mobilizing calcium
• mast cells fail to migrate in response to histamine stimulation unlike wild-type mast cells




Genotype
MGI:3775271
ht3
Allelic
Composition		 Hrh4tm1Lex/Hrh4+
Genetic
Background		 involves: 129S/SvEv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hrh4tm1Lex mutation (1 available); any Hrh4 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal mast cell physiology ( J:132140 )
• mast cells do not respond to 30 uM histamine stimulation by mobilizing calcium as strongly as wild-type cells

hematopoietic system
abnormal mast cell physiology ( J:132140 )
• mast cells do not respond to 30 uM histamine stimulation by mobilizing calcium as strongly as wild-type cells




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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory