Phenotypes associated with this allele

• Allele Symbol: Rnd3^{Gt(OST364657)Lex}
• Allele Name: gene trap OST364657, Lexicon Genetics
• Allele ID: MGI:3530138
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rnd3^Gt(OST364657)Lex/Rnd3^Gt(OST364657)Lex	involves: 129S5/SvEvBrd * C57BL/6J	MGI:5009345

Genotype
MGI:5009345

hm1

• Allelic Composition: Rnd3^{Gt(OST364657)Lex}/Rnd3^{Gt(OST364657)Lex}
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Smaller size and abnormal position of the hindlimbs in Rnd3^{Gt(OST364657)Lex}/Rnd3^{Gt(OST364657)Lex} mice

mortality/aging

premature death ( J:172361 )

• do not survive beyond P29

postnatal lethality, incomplete penetrance ( J:172361 )

• median survival of 15 days

growth/size/body

decreased birth body size ( J:172361 )

weight loss ( J:172361 )

• start losing weight around P18

postnatal growth retardation ( J:172361 )

slow postnatal weight gain ( J:172361 )

behavior/neurological

abnormal motor capabilities/coordination/movement ( J:172361 )

• impaired performance in multiple motor tests, including vertical climbing and clinging, righting reflex and negative geotaxis

abnormal pinna reflex ( J:172361 )

• delayed development

impaired righting response ( J:172361 )

limb grasping ( J:172361 )

• at P21

abnormal placing response ( J:172361 )

• delay in the appearance of the placing response

decreased grip strength ( J:172361 )

• reduced latency to fall in a wire suspension test at P13, P14, and P15

abnormal limb posture ( J:172361 )

• hyperextension of the feet and inability to flex the feet

abnormal locomotor behavior ( J:172361 )

• immature pivoting locomotion persists longer compared to wild-type controls indicating a possible delay in cranio-caudal maturation

• latency to start walking is significantly higher in the first 10 days of life compared to wild-type controls

• at P3 and P4 mice are unable to stand on all fours and walk in a straight line in 60 s while wild-type controls complete the task in under 40 s

ataxia ( J:172361 )

• general ataxia

abnormal gait ( J:172361 )

• hindlimb steps are shorter and irregular

short stride length ( J:172361 )

decreased locomotor activity ( J:172361 )

positive geotaxis ( J:172361 )

behavioral developmental delay ( J:172361 )

• delay in the disappearance of the rooting response and crossing extensor reflex

• delay in the appearance of the grasping response

delayed suckling reflex loss ( J:172361 )

• delay in the disappearance of the rooting response

convulsive seizures ( J:172361 )

• spontaneous convulsions

nervous system

convulsive seizures ( J:172361 )

• spontaneous convulsions

decreased motor neuron number ( J:172361 )

• reduction of 43% in the ventral spinal cord compared to wild-type controls

abnormal neuromuscular synapse morphology ( J:172361 )

• less developed compared to control littermates

• delay in neuromuscular junction maturation with an increased presence of less developed plates, both in the forelimbs and in the hindlimbs

abnormal spinal nerve morphology ( J:172361 )

• complete absence of the common peroneal nerve

abnormal sciatic nerve morphology ( J:172361 )

• the sciatic nerve fails to split into the common peroneal and the tibial nerves and has only the tibial component

muscle

abnormal hypaxial muscle morphology ( J:172361 )

• atrophy of muscles normally targeted by the common peroneal nerve, with most of the muscle fibers replaced by adipose and connective tissue

abnormal tibialis anterior morphology ( J:172361 )

• almost all muscle cells are replaced by adipose tissue and clusters of small size fibers

hearing/vestibular/ear

abnormal ear development ( J:172361 )

• permeation of the auditory conduct and development of the pinna reflex take place later

vision/eye

delayed eyelid opening ( J:172361 )

limbs/digits/tail

abnormal tibialis anterior morphology ( J:172361 )

• almost all muscle cells are replaced by adipose tissue and clusters of small size fibers