Phenotypes associated with this allele

• Allele Symbol: Tg(Myog-cre)1Eno
• Allele Name: transgene insertion 1, Eric N Olson
• Allele ID: MGI:3530558
• Summary: 18 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Hdac4^tm1Eno/Hdac4^tm2.1Eno Hdac5^tm1Eno/Hdac5^tm1Eno Tg(Myog-cre)1Eno/0	involves: 129 * 129S/SvEv * 129S2/SvPas	MGI:4418137
cn2	Ppargc1a^tm2.1Brsp/Ppargc1a^tm2.1Brsp Tg(Myog-cre)1Eno/0	involves: 129 * BALB/c * C57BL/6J * C57BL/6N	MGI:5576914
cn3	Obscn^tm1Chen/Obscn^tm1Chen Obsl1^tm1.1Slan/Obsl1^tm1.1Slan Tg(Myog-cre)1Eno/0	involves: 129 * Black Swiss * C57BL/6 * SJL	MGI:6476968
cn4	Obsl1^tm1.1Slan/Obsl1^tm1.1Slan Tg(Myog-cre)1Eno/0	involves: 129 * Black Swiss * C57BL/6 * SJL	MGI:6476965
cn5	Ppargc1a^tm2Brsp/Ppargc1a^+ Tg(Myog-cre)1Eno/0	involves: 129 * C57BL/6	MGI:5576900
cn6	Cygb^tm1Ppam/Cygb^tm1Ppam Tg(Myog-cre)1Eno/0	involves: 129 * C57BL/6	MGI:5575857
cn7	Ppargc1a^tm1Brsp/Ppargc1a^tm2Brsp Tg(Myog-cre)1Eno/0	involves: 129 * C57BL/6 * FVB/N	MGI:5576899
cn8	Srf^tm2.1Nor/Srf^tm2.1Nor Tg(Myog-cre)1Eno/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3530886
cn9	Mbnl1^tm1Sws/Mbnl1^tm1Sws Mbnl2^tm1Sws/Mbnl2^tm1Sws Tg(Myog-cre)1Eno/0	involves: 129S1/Sv * 129S1/SvImJ * C57BL	MGI:5616749
cn10	Acacb^tm1.1Lowl/Acacb^tm1.1Lowl Tg(Myog-cre)1Eno/0	involves: 129S4/SvJaeSor * 129S6/SvEvTac	MGI:4450935
cn11	Mapk14^tm1Lex/Mapk14^tm1Lex Tg(Myog-cre)1Eno/0	involves: 129S5/SvEvBrd	MGI:4415790
cn12	Pnpla2^tm1Eek/Pnpla2^tm1Eek Tg(Myog-cre)1Eno/0	involves: 129S * C57BL/6	MGI:5554580
cn13	Hdac4^tm2.1Eno/Hdac4^tm2.1Eno Tg(Myog-cre)1Eno/0	involves: 129S/SvEv	MGI:4418186
cn14	Mef2c^tm1Eno/Mef2c^tm2Eno Tg(Myog-cre)1Eno/0	involves: 129S/SvEv * 129S7/SvEvBrd	MGI:4418148
cn15	Mapk12^tm1Lex/Mapk12^tm1Lex Tg(Myog-cre)1Eno/0	involves: 129S/SvEvBrd	MGI:4415789
cn16	Mapk11^tm2Lex/Mapk11^tm2Lex Tg(Myog-cre)1Eno/0	involves: 129S/SvEvBrd	MGI:4415788
cn17	Gnai2^tm2.1Lbi/Gnai2^tm2.1Lbi Gnai3^tm1Lbi/Gnai3^tm1Lbi Tg(Myog-cre)1Eno/0	involves: 129S/SvEv * C57BL/6	MGI:5471660
cn18	Mef2d^tm1Eno/Mef2d^tm1Eno Tg(Myog-cre)1Eno/0	Not Specified	MGI:4418150

Genotype
MGI:4418137

cn1

• Allelic Composition: Hdac4^tm1Eno/Hdac4^tm2.1Eno; Hdac5^tm1Eno/Hdac5^tm1Eno; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129 * 129S/SvEv * 129S2/SvPas

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

muscle

abnormal skeletal muscle fiber type ratio ( J:127416 )

• soleus muscles show an increase in the percentage of slow myofibers

Genotype
MGI:5576914

cn2

• Allelic Composition: Ppargc1a^tm2.1Brsp/Ppargc1a^tm2.1Brsp; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129 * BALB/c * C57BL/6J * C57BL/6N

homeostasis/metabolism

decreased circulating glucose level ( J:208380 )

• at 3 and 12 months in fasting mice

decreased circulating insulin level ( J:208380 )

• at 3 and 24 months

decreased respiratory quotient ( J:208380 )

• in the dark phase only

impaired glucose tolerance ( J:208380 )

• in aged mice

• however, glucose tolerance is normal at 3 and 12 months

insulin resistance ( J:208380 )

• in aged mice

• however, insulin resistance is normal at 6 months

growth/size/body

growth/size/body region phenotype ( J:208380 )

N • mice exhibit normal body weight

decreased lean body mass ( J:208380 )

• in aged mice

cellular

abnormal mitochondrial physiology ( J:208380 )

• decreased succinate dehydrogenase activity in the gastrocnemius of young and old mice

adipose tissue

increased percent body fat/body weight ( J:208380 )

• in aged mice

Genotype
MGI:6476968

cn3

• Allelic Composition: Obscn^tm1Chen/Obscn^tm1Chen; Obsl1^tm1.1Slan/Obsl1^tm1.1Slan; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129 * Black Swiss * C57BL/6 * SJL

mortality/aging

mortality/aging ( J:283623 )

N • mice are viable and develop normally

muscle

muscle phenotype ( J:283623 )

N • analysis of myofilament structure using sarcomeric alpha-actinin 2 indicated normal sarcomerogenesis in tibialis anterior (TA) muscles

N • immunofluorescence analysis of sarcomeric alpha-actinin 2 and titin-M8 localization revealed that Z-disc and M-band structures are not significantly altered

N • at 4 months of age, muscle weight to tibia-length ratios for TA, soleus, gastrocnemius and extensor digitorum longus (EDL) muscles are normal

N • no significant changes are observed in the number of centralized nuclei in TA muscle at 4 months of age

abnormal sarcolemma morphology ( J:283623 )

• skeletal muscles show a slight decrease in protein levels of utrophin and other dystrophin-sarcoglycan (DSG) components, including alpha-dystrobrevin, whereas levels of tubulin are slightly increased

• although overall dystrophin levels are normal, subsarcolemmal dystrophin localization in longitudinal sections of TA muscles is more patchy and abnormally distributed than in single Obscn^tm1Chen knockouts, indicating exacerbated membrane fragility

• a significant portion of TA muscle fibers are mouse IgG-positive, unlike in single Obscn^tm1Chen knockouts or Obsl1 skeletal muscle-knockouts, suggesting impaired sarcolemmal integrity

• increased membrane fragility leads to upregulation of dysferlin and filamin C proteins (involved in muscle repair), reduced caveolin-1 and TRPC1 protein levels, but normal levels of neuronal nitric oxidase (nNOS) and L-type calcium channel DHPR alpha-2 subunit, indicating potential changes to DSG-associated, but not T-tubule-based ion channels

abnormal sarcoplasmic reticulum morphology ( J:283623 )

• TA muscles show a decrease in small ankyrin-1.5 (sAnk1.5) protein levels similar to that in single Obscn^tm1Chen knockouts

• skeletal muscles show alterations to levels of lumenal SR calcium binding proteins (sarcalumenin and Casq2) as well as sarcoendoplasmic reticulum Ca2+ ATPase (Serca)

• proteome and expression data revealed increases in junctional SR proteins like triadin, junction and ryanodine receptors

decreased skeletal muscle fiber diameter ( J:283623 )

• analysis of cross-sectional areas of TA muscle revealed a decrease in fiber size

abnormal muscle physiology ( J:283623 )

• analysis of twitch parameters in EDL muscles revealed increased time-to-peak (TtP) values relative to control and Obsl1 skeletal muscle-knockouts

• however, half-relaxation times remain normal

homeostasis/metabolism

abnormal enzyme/coenzyme level ( J:283623 )

• monoamine oxidase A and B levels are significantly decreased in TA muscles

increased catalase level ( J:283623 )

• catalase levels are significantly increased in soleus muscle

abnormal glycogen catabolism ( J:283623 )

• muscle glycogen phosphorylase, the rate determining enzyme responsible for glycogen breakdown, is significantly decreased

cellular

abnormal mitochondrial ATP synthesis coupled electron transport ( J:283623 )

• almost all identified mitochondrial electron transport chain proteins are downregulated in soleus and TA muscles

• analysis of whole TA and/or soleus muscle extracts using oxphos antibody cocktail or a Uqcrb antibody revealed slightly or significantly reduced levels of mitochondrial complex I, II, III, IV, and V proteins

reproductive system

reproductive system phenotype ( J:283623 )

N • mice are fertile

Genotype
MGI:6476965

cn4

• Allelic Composition: Obsl1^tm1.1Slan/Obsl1^tm1.1Slan; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129 * Black Swiss * C57BL/6 * SJL

mortality/aging

mortality/aging ( J:283623 )

N • mice do not exhibit premature death or gross morphological abnormalities

muscle

muscle phenotype ( J:283623 )

N • mice exhibit normal sarcomerogenesis in tibialis anterior (TA) muscles; Z-disc and M-band structures are not significantly altered

N • no significant changes are observed in the number of centralized nuclei in TA muscle at 4 months of age

increased extensor digitorum longus weight ( J:283623 )

• at 4 months of age, muscle weight to tibia-length ratio is slightly increased for extensor digitorum longus (EDL) muscles

• however, muscle weight to tibia-length ratios for TA, soleus, and gastrocnemius are normal

increased skeletal muscle fiber diameter ( J:283623 )

• analysis of cross-sectional areas in TA muscles revealed an increase in fiber size

limbs/digits/tail

increased extensor digitorum longus weight ( J:283623 )

• at 4 months of age, muscle weight to tibia-length ratio is slightly increased for extensor digitorum longus (EDL) muscles

• however, muscle weight to tibia-length ratios for TA, soleus, and gastrocnemius are normal

Genotype
MGI:5576900

cn5

• Allelic Composition: Ppargc1a^tm2Brsp/Ppargc1a⁺; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129 * C57BL/6

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:127403 )

N • mice fed a high fat diet exhibit normal heat production and oxygen consumption

increased circulating glucose level ( J:127403 )

• in mice fed a high fat diet

• however, mice fed standard chow exhibit normal glucose serum levels

increased circulating interleukin-6 level ( J:127403 )

impaired glucose tolerance ( J:127403 )

• in mice fed standard chow or a high fat diet

immune system

increased circulating interleukin-6 level ( J:127403 )

behavior/neurological

behavior/neurological phenotype ( J:127403 )

N • mice fed a high fat diet exhibit normal food intake

growth/size/body

growth/size/body region phenotype ( J:127403 )

N • mice fed standard chow or a high fat diet exhibit normal body weight and composition

Genotype
MGI:5575857

cn6

• Allelic Composition: Cygb^tm1Ppam/Cygb^tm1Ppam; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129 * C57BL/6

Delayed regeneration of muscle in Cygb^tm1Ppam/Cygb^tm1Ppam Tg(Myog-cre)1Eno/0 mice after cardiotoxin injury

muscle

abnormal myoblast differentiation ( J:206378 )

• muscle progenitor cells are unable to differentiate fully in vitro

• full differentiation into myotubes is impaired in vitro

skeletal muscle degeneration ( J:206378 )

• prolonged muscle degeneration at 21 days after cardiotoxin-induced injury

abnormal muscle precursor cell physiology ( J:206378 )

• muscle progenitor cell proliferation is impaired in vitro

impaired muscle regeneration ( J:206378 )

• 14 days postinjury induced by cardiotoxin

cellular

increased apoptosis ( J:206378 )

• increase both in duration and magnitude of myocyte death after cardiotoxin-induced injury

abnormal myoblast differentiation ( J:206378 )

• muscle progenitor cells are unable to differentiate fully in vitro

• full differentiation into myotubes is impaired in vitro

decreased cell proliferation ( J:206378 )

• muscle progenitor cell proliferation is impaired in vitro

oxidative stress ( J:206378 )

• in muscles at 21 days post cardiotoxin-induced injury

Genotype
MGI:5576899

cn7

• Allelic Composition: Ppargc1a^tm1Brsp/Ppargc1a^tm2Brsp; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129 * C57BL/6 * FVB/N

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:127403 )

N • mice fed standard chow or a high fat diet exhibit normal insulin tolerance

N • hepatic glucose output under basal and clamped conditions is normal

decreased susceptibility to diet-induced obesity ( J:127403 )

• in mice fed a high fat diet

increased circulating interleukin-6 level ( J:127403 )

increased oxygen consumption ( J:127403 )

• in mice fed a high fat diet

decreased respiratory quotient ( J:127403 )

• in mice fed a high fat diet

abnormal glucose homeostasis ( J:127403 )

• mice exhibit increased systemic glucose uptake and glycolysis compared with control mice

• however, glycogen synthesis is normal

increased circulating glucose level ( J:127403 )

• in fed mice on standard chow

• however, fasted mice exhibit normal blood glucose levels

hyperglycemia ( J:127403 )

• in fasted and fed mice on a high fat diet

decreased circulating insulin level ( J:127403 )

• in fed mice on standard chow or a high fat diet

impaired glucose tolerance ( J:127403 )

• in fed mice on a high fat diet

increased basal metabolism ( J:127403 )

• in mice fed a high fat diet

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:127403 )

N • islets exhibit normal basal and glucose-stimulated insulin secretion

abnormal pancreatic beta cell morphology ( J:127403 )

• reduced cell size

increased pancreatic beta cell mass ( J:127403 )

enlarged pancreatic islets ( J:127403 )

growth/size/body

decreased body weight ( J:127403 )

• in mice fed standard chow or a high fat diet

decreased susceptibility to diet-induced obesity ( J:127403 )

• in mice fed a high fat diet

immune system

increased circulating interleukin-6 level ( J:127403 )

adipose tissue

decreased percent body fat/body weight ( J:127403 )

• in mice fed standard chow or a high fat diet

cellular

abnormal mitochondrial physiology ( J:127403 )

• decreased function as measured by Cox and succinate dehydrogenase activity

behavior/neurological

decreased food intake ( J:127403 )

• during the day and night in mice fed a high fat diet

Genotype
MGI:3530886

cn8

• Allelic Composition: Srf^tm2.1Nor/Srf^tm2.1Nor; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

Curved spine and cyanosis in Srf^tm2.1Nor/Srf^tm2.1Nor Tg(Myog-cre)1Eno/0 mice

mortality/aging

neonatal lethality, complete penetrance ( J:96122 )

• mutants die after birth from an inability to breathe

behavior/neurological

no spontaneous movement ( J:96122 )

• mutants are immobile at birth

growth/size/body

decreased birth weight ( J:96122 )

• mutants weigh less at birth as a result of decreased muscle mass

homeostasis/metabolism

cyanosis ( J:96122 )

muscle

abnormal skeletal muscle fiber morphology ( J:96122 )

• muscle fibers are thinner than normal with large interstitial spaces however the normal number of nuclei are seen indicating a decrease in growth but not proliferation of the cells

• the sarcomere units are smaller and the fibers are narrow and disorganized

abnormal diaphragm morphology ( J:96122 )

• abnormalities in the diaphragm muscle prevent breathing

decreased skeletal muscle mass ( J:96122 )

respiratory system

respiratory failure ( J:96122 )

• neonates are unable to breathe

skeleton

kyphosis ( J:96122 )

cardiovascular system

cardiovascular system phenotype ( J:96122 )

N • no cardiac abnormalities are seen and the heart is beating at birth

Genotype
MGI:5616749

cn9

• Allelic Composition: Mbnl1^tm1Sws/Mbnl1^tm1Sws; Mbnl2^tm1Sws/Mbnl2^tm1Sws; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129S1/Sv * 129S1/SvImJ * C57BL

growth/size/body

decreased birth body size ( J:218011 )

behavior/neurological

abnormal motor capabilities/coordination/movement ( J:218011 )

• develop severe motor deficits beginning at 12 weeks of age

decreased grip strength ( J:218011 )

skeleton

kyphosis ( J:218011 )

• develop kyphosis beginning at 12 weeks of age

muscle

decreased skeletal muscle fiber size ( J:218011 )

increased variability of skeletal muscle fiber size ( J:218011 )

centrally nucleated skeletal muscle fibers ( J:218011 )

skeletal muscle degeneration ( J:218011 )

• profound deficiency of adult skeletal muscle, with severe muscle pathology including small myofibers, fiber size heterogeneity, and centralized nuclei in nearly all myofibers

Genotype
MGI:4450935

cn10

• Allelic Composition: Acacb^tm1.1Lowl/Acacb^tm1.1Lowl; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129S4/SvJaeSor * 129S6/SvEvTac

normal phenotype

no abnormal phenotype detected ( J:159285 )

• no effect on bodyweight, food intake, or body composition

• little effect on ambient glucose and insulin levels

Genotype
MGI:4415790

cn11

• Allelic Composition: Mapk14^tm1Lex/Mapk14^tm1Lex; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129S5/SvEvBrd

cardiovascular system

cardiovascular system phenotype ( J:155408 )

N • mice exhibit normal angiogenesis in response to endurance exercise

cellular

cellular phenotype ( J:155408 )

N • mice exhibit normal mitochondrial biogenesis in response to endurance exercise

muscle

muscle phenotype ( J:155408 )

N • mice exhibit normal fiber-type transformation in response to endurance exercise

Genotype
MGI:5554580

cn12

• Allelic Composition: Pnpla2^tm1Eek/Pnpla2^tm1Eek; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129S * C57BL/6

homeostasis/metabolism

abnormal lipid level ( J:207007 )

• increase in diacylglycerol levels (specifically subspecies C14:0/16:0, C14:0/18:0, C16:0/18:0) in skeletal muscle in mice fed both chow and high fat diet as compared to control

increased fatty acids level ( J:207007 )

• increase in fatty acid-CoA levels (specifically subspecies C14:0, C16:0) in mice fed both chow and high fat diet as compared to controls

increased skeletal muscle triglyceride level ( J:207007 )

• increase in intramyocellular triacylglycerol (IMTG) levels in skeletal muscle; increase is specific to muscle fiber type (2A>2X>1, but not 2B)

• IMTG levels are increased in mice fed both chow and high fat diet as compared to controls

muscle

increased skeletal muscle triglyceride level ( J:207007 )

• increase in intramyocellular triacylglycerol (IMTG) levels in skeletal muscle; increase is specific to muscle fiber type (2A>2X>1, but not 2B)

• IMTG levels are increased in mice fed both chow and high fat diet as compared to controls

Genotype
MGI:4418186

cn13

• Allelic Composition: Hdac4^tm2.1Eno/Hdac4^tm2.1Eno; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129S/SvEv

nervous system

abnormal motor nerve collateral sprouting ( J:155806 )

• reinnervation occurs more rapidly compared to controls after nerve crush or cut

• however, in the absence of injury no defects in neuromuscular junctions are detected

Genotype
MGI:4418148

cn14

• Allelic Composition: Mef2c^tm1Eno/Mef2c^tm2Eno; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129S/SvEv * 129S7/SvEvBrd

muscle

abnormal skeletal muscle fiber type ratio ( J:127416 )

• reduction in slow fibers within the soleus

• loss of type I fibers in the gastrocnemius and plantaris muscles and a decrease in number and intensity of type I fibers in the soleus

Genotype
MGI:4415789

cn15

• Allelic Composition: Mapk12^tm1Lex/Mapk12^tm1Lex; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129S/SvEvBrd

cardiovascular system

decreased angiogenesis ( J:155408 )

• marker expression indicates that exercise-induced angiogenesis is decreased compared to in similarly treated wild-type mice

cellular

decreased mitochondrial fission ( J:155408 )

• marker expression indicates that exercise-induced mitochondrial biogenesis is decreased compared to in similarly treated wild-type mice

muscle

muscle phenotype ( J:155408 )

N • mice exhibit normal fiber-type transformation in response to endurance exercise

Genotype
MGI:4415788

cn16

• Allelic Composition: Mapk11^tm2Lex/Mapk11^tm2Lex; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129S/SvEvBrd

cardiovascular system

cardiovascular system phenotype ( J:155408 )

N • mice exhibit normal angiogenesis in response to endurance exercise

cellular

cellular phenotype ( J:155408 )

N • mice exhibit normal mitochondrial biogenesis in response to endurance exercise

muscle

muscle phenotype ( J:155408 )

N • mice exhibit normal fiber-type transformation in response to endurance exercise

Genotype
MGI:5471660

cn17

• Allelic Composition: Gnai2^tm2.1Lbi/Gnai2^tm2.1Lbi; Gnai3^tm1Lbi/Gnai3^tm1Lbi; Tg(Myog-cre)1Eno/0
• Genetic Background: involves: 129S/SvEv * C57BL/6

Rib fusion phenotype is enhanced in Gnai3^tm1Lbi/Gnai3^tm1Lbi mice by loss of Gnai2 in cartilage

skeleton

rib fusion ( J:193170 )

• rib fusions are similar in severity to mice homozygous for Gnai3^tm1Lbi alone

Genotype
MGI:4418150

cn18

• Allelic Composition: Mef2d^tm1Eno/Mef2d^tm1Eno; Tg(Myog-cre)1Eno/0
• Genetic Background: Not Specified

muscle

abnormal skeletal muscle fiber type ratio ( J:127416 )

• slow fibers within the soleus are reduced