Phenotypes associated with this allele

• Allele Symbol: Pknox1^{Gt(OST71835)Lex}
• Allele Name: gene trap OST71835, Lexicon Genetics
• Allele ID: MGI:3530910
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pknox1^Gt(OST71835)Lex/Pknox1^Gt(OST71835)Lex	B6.129S5-Pknox1^Gt(OST71835)Lex	MGI:4460937
hm2	Pknox1^Gt(OST71835)Lex/Pknox1^Gt(OST71835)Lex	involves: 129S5/SvEvBrd	MGI:5469882
hm3	Pknox1^Gt(OST71835)Lex/Pknox1^Gt(OST71835)Lex	involves: 129S5/SvEvBrd * C57BL/6	MGI:3611267
hm4	Pknox1^Gt(OST71835)Lex/Pknox1^Gt(OST71835)Lex	involves: 129S5/SvEvBrd * C57BL/6J	MGI:3609796

Genotype
MGI:4460937

hm1

• Allelic Composition: Pknox1^{Gt(OST71835)Lex}/Pknox1^{Gt(OST71835)Lex}
• Genetic Background: B6.129S5-Pknox1^{Gt(OST71835)Lex}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:111418 )

• mice die between E17.5 and P0

cardiovascular system

decreased angiogenesis ( J:111418 )

• at E10.5, mice exhibit a decreased vascular tree at the level of the head and intersomitic vessels compared with wild-type mice

• in culture, E7.5 to E7.75 allantois cells exhibit reduced angiogenesis with fewer capillaries and reduced vasculature complexity compared with wild-type cells

hemorrhage ( J:111418 )

• in 13% of mice

vision/eye

abnormal eye morphology ( J:111418 )

• in 62% of mice at E14.5

abnormal retina pigment epithelium morphology ( J:111418 )

• mice exhibit anomalies and duplications compared with wild-type mice

small lens ( J:111418 )

• in 40% of mice

abnormal eye development ( J:111418 )

• 14% of mice exhibit eyes that are still encased deep within the head unlike wild-type mice

abnormal retina neuronal layer morphology ( J:111418 )

• in 23% of mice

hematopoietic system

abnormal erythropoiesis ( J:111418 )

• at E15.5, mice exhibit a decrease in total fetal liver cells, an increase in early progenitor subpopulations R2 and R3, and a decrease in the R4 subpopulation compared with wild-type mice

• at E16.5, mice exhibit an increased difference in progenitor cells compared with wild-type mice

anemia ( J:111418 )

• at E16.5

decreased erythroid progenitor cell number ( J:111418 )

• generated from fetal liver cells

decreased hematocrit ( J:111418 )

• at E16.5

increased nucleated erythrocyte cell number ( J:111418 )

• at E16.5

homeostasis/metabolism

edema ( J:111418 )

• in 67% of mice at E15.5

growth/size/body

abnormal prenatal growth/weight/body size ( J:111418 )

• at E15.5, mice exhibit general organ hypoplasia compared with wild-type mice

decreased fetal size ( J:111418 )

• in 75% of mice at E15.5

liver/biliary system

abnormal liver development ( J:111418 )

• 71% of mice exhibit smaller liver spots than in wild-type mice

pigmentation

abnormal retina pigment epithelium morphology ( J:111418 )

• mice exhibit anomalies and duplications compared with wild-type mice

integument

pallor ( J:111418 )

• in 75% of mice at E15.5

Genotype
MGI:5469882

hm2

• Allelic Composition: Pknox1^{Gt(OST71835)Lex}/Pknox1^{Gt(OST71835)Lex}
• Genetic Background: involves: 129S5/SvEvBrd

hematopoietic system

abnormal blood cell morphology/development ( J:192251 )

• fetal livers cells are less competent in transplantation assays than wild-type cells

Genotype
MGI:3611267

hm3

• Allelic Composition: Pknox1^{Gt(OST71835)Lex}/Pknox1^{Gt(OST71835)Lex}
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:103769 )

• variable penetrance of lethality is seen after E17.5 but before P0 with only 6% homozygous offspring from heterozygous crosses instead of the expected 25%

immune system

increased T cell apoptosis ( J:103769 )

• the absolute number of double positive T cells in the thymus is similar to wild-type; however apoptosis of double positive T cells is increased

thymus hypoplasia ( J:103769 )

• thymic cellularity is reduced after 12 weeks of age by up to 30% and this is more evident in males

increased double-negative T cell number ( J:103769 )

• in the thymus the number of double negative progenitors is increased

decreased single-positive T cell number ( J:103769 )

• the percentage of CD4+ or CD8+ cells is reduced by 40% in the spleen and blood and by 20-30% in the lymph nodes

• the absolute number of mature CD4+ and CD8+ cells in the lymph nodes is reduced by more than 50%

• in the thymus the number of single positive T cells is reduced 2- to 3-fold

growth/size/body

decreased birth body size ( J:103769 )

• homozygotes are smaller at birth but catch up to littermates by 2 to 3 months of age

hematopoietic system

increased T cell apoptosis ( J:103769 )

• the absolute number of double positive T cells in the thymus is similar to wild-type; however apoptosis of double positive T cells is increased

thymus hypoplasia ( J:103769 )

• thymic cellularity is reduced after 12 weeks of age by up to 30% and this is more evident in males

increased double-negative T cell number ( J:103769 )

• in the thymus the number of double negative progenitors is increased

decreased single-positive T cell number ( J:103769 )

• the percentage of CD4+ or CD8+ cells is reduced by 40% in the spleen and blood and by 20-30% in the lymph nodes

• the absolute number of mature CD4+ and CD8+ cells in the lymph nodes is reduced by more than 50%

• in the thymus the number of single positive T cells is reduced 2- to 3-fold

cellular

increased T cell apoptosis ( J:103769 )

• the absolute number of double positive T cells in the thymus is similar to wild-type; however apoptosis of double positive T cells is increased

endocrine/exocrine glands

thymus hypoplasia ( J:103769 )

• thymic cellularity is reduced after 12 weeks of age by up to 30% and this is more evident in males

Genotype
MGI:3609796

hm4

• Allelic Composition: Pknox1^{Gt(OST71835)Lex}/Pknox1^{Gt(OST71835)Lex}
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J

growth/size/body

decreased body weight ( J:103485 )

• mean total tissue mass is reduced in male homozygotes

adipose tissue

decreased percent body fat/body weight ( J:103485 )

• mean percent total body fat is reduced in male homozygotes