Phenotypes associated with this allele

• Allele Symbol: Pou5f1^tm1.1Scho
• Allele Name: targeted mutation 1.1, Hans R Shoeler
• Allele ID: MGI:3531175
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Alpl^tm1(cre)Nagy/Alpl^+ Pou5f1^tm1Scho/Pou5f1^tm1.1Scho	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3579642

Genotype
MGI:3579642

cn1

• Allelic Composition: Alpl^tm1(cre)Nagy/Alpl⁺; Pou5f1^tm1Scho/Pou5f1^tm1.1Scho
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

oligozoospermia ( J:95317 )

♂ • variable depletion of spermatogenic cells is seen with testes containing both germ cell free seminiferous tubules and unaffected tubules

♂ • the percentage of germ cell free tubules varied from 30% to 100%; however unlike females gem cell depletion did no increase with age

decreased primordial germ cell number ( J:95317 )

• at E10.5 and E12.5 the late migratory and postmigratory primordial germ cell populations are reduced; however the size of the premigratory (E6.5) and early migratory (E9.5) primordial germ cell populations are normal

• up to 70% of primordial germ cells undergo apoptosis before colonizing the gonadal ridges

reproductive system

oligozoospermia ( J:95317 )

♂ • variable depletion of spermatogenic cells is seen with testes containing both germ cell free seminiferous tubules and unaffected tubules

♂ • the percentage of germ cell free tubules varied from 30% to 100%; however unlike females gem cell depletion did no increase with age

decreased primordial germ cell number ( J:95317 )

• at E10.5 and E12.5 the late migratory and postmigratory primordial germ cell populations are reduced; however the size of the premigratory (E6.5) and early migratory (E9.5) primordial germ cell populations are normal

• up to 70% of primordial germ cells undergo apoptosis before colonizing the gonadal ridges

decreased primordial ovarian follicle number ( J:95317 )

♀ • at 3 weeks of age the number of primordial follicles is reduced 25 - 100-fold

absent ovarian follicles ( J:95317 )

♀ • at 6 weeks of age hardly any primordial follicles and very few growing follicles are seen

♀ • after 6 weeks of age ovarian follicles are mostly absent

small seminiferous tubules ( J:95317 )

♂ • seminiferous tubules lacking germ cells are reduced in diameter

increased Leydig cell number ( J:95317 )

♂ • seen around seminiferous tubules lacking germ cells

female infertility ( J:95317 )

♀

reduced male fertility ( J:95317 )

♂ • fertility is variably impaired depending on the efficiency of Cre recombination (averaging about 60%)

♂ • males that do produce offspring pass on the unrecombined allele

endocrine/exocrine glands

decreased primordial ovarian follicle number ( J:95317 )

♀ • at 3 weeks of age the number of primordial follicles is reduced 25 - 100-fold

absent ovarian follicles ( J:95317 )

♀ • at 6 weeks of age hardly any primordial follicles and very few growing follicles are seen

♀ • after 6 weeks of age ovarian follicles are mostly absent

small seminiferous tubules ( J:95317 )

♂ • seminiferous tubules lacking germ cells are reduced in diameter

increased Leydig cell number ( J:95317 )

♂ • seen around seminiferous tubules lacking germ cells

embryo

abnormal gonadal ridge morphology ( J:95317 )

• clusters of apoptotic primordial germ cells are detected on the genital ridge at E10.5