Phenotypes associated with this allele

• Allele Symbol: Tg(Camk2a-cre)T50Stl
• Allele Name: transgene insertion T50, Susumu Tonegawa
• Allele ID: MGI:3531299
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nr3c1^tm2Ljm/Nr3c1^tm2Ljm Tg(Camk2a-cre)T50Stl/?	involves: 129 * BALB/c * C57BL/6 * CBA	MGI:3716826
cn2	Fos^tm1Mxu/Fos^tm1Mxu Tg(Camk2a-cre)T50Stl/0	involves: 129S2/SvPas * BALB/c * C57BL/6	MGI:3814562
cn3	Pten^tm1Hwu/Pten^tm1Hwu Tg(Camk2a-cre)T50Stl/0 Tg(Thy1-EGFP)MJrs/0	involves: 129S4/SvJae * BALB/c * C57BL/6J * CBA	MGI:4942301
cn4	Gabrg2^tm2Lusc/Gabrg2^tm2Lusc Tg(Camk2a-cre)T50Stl/0	involves: 129X1/SvJ * BALB/c * C57BL/6	MGI:2680635
cn5	Bdnf^tm1Limm/Bdnf^tm1Limm Tg(Camk2a-cre)T50Stl/0	involves: BALB/c * C57BL/6	MGI:3770148

Genotype
MGI:3716826

cn1

• Allelic Composition: Nr3c1^tm2Ljm/Nr3c1^tm2Ljm; Tg(Camk2a-cre)T50Stl/?
• Genetic Background: involves: 129 * BALB/c * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

increased circulating corticosterone level ( J:96260 )

• peak and basal corticosterone levels are increased at 6 months of age

behavior/neurological

behavioral despair ( J:96260 )

• in forced swim and tail suspension tests mice exhibit a decrease in activity indicative of despair

• however, despair behaviors are reversed by chronic treatment with the tricyclic antidepressant imipramine

decreased locomotor activity ( J:96260 )

• in forced swim and tail suspension tests mice exhibit a decrease in activity indicative of despair

nervous system

absent long-term depression ( J:96260 )

• low-frequency stimulation in the presence of 1um corticosterone of hippocampal slices can not induce long term depression (LTD)

Genotype
MGI:3814562

cn2

• Allelic Composition: Fos^tm1Mxu/Fos^tm1Mxu; Tg(Camk2a-cre)T50Stl/0
• Genetic Background: involves: 129S2/SvPas * BALB/c * C57BL/6

nervous system

abnormal nervous system electrophysiology ( J:108041 )

• population spike amplitude evoked by minimal stimulation intensity is significantly larger in hippocampal mutant slices compared to wild-type slices with a large increase in cellular excitability and a larger maximal response

• >60% of slices show epileptiform activity following low intensity stimulation compared to <40% of wild-type

• very low concentration kainic acid (KA) stimulation of hippocampal slices produces maximal CA3 population spike amplitude and higher concentrations induces severe epileptiform activity, reducing amplitude of the population response

Genotype
MGI:4942301

cn3

• Allelic Composition: Pten^tm1Hwu/Pten^tm1Hwu; Tg(Camk2a-cre)T50Stl/0; Tg(Thy1-EGFP)MJrs/0
• Genetic Background: involves: 129S4/SvJae * BALB/c * C57BL/6J * CBA

nervous system

increased brain size ( J:145970 )

• increase in brain size due to expansion of allocortex and neocortex

abnormal dendrite morphology ( J:145970 )

• apical dendrites of pyramidal neurons in cortical layer 2/3 but not layer 5 are longer and more tortuous than in controls

• total arbor length is on average 1.6 mm longer in mutants, indicating an approximate 80% expansion of the apical dendritic tree

• however, basal dendritic length is not different

• rapamycin treatments completely prevent dendritic growth seen following Pten deletion

Genotype
MGI:2680635

cn4

• Allelic Composition: Gabrg2^tm2Lusc/Gabrg2^tm2Lusc; Tg(Camk2a-cre)T50Stl/0
• Genetic Background: involves: 129X1/SvJ * BALB/c * C57BL/6

mortality/aging

premature death ( J:86221 )

• mice die around 7 weeks of age

Genotype
MGI:3770148

cn5

• Allelic Composition: Bdnf^tm1Limm/Bdnf^tm1Limm; Tg(Camk2a-cre)T50Stl/0
• Genetic Background: involves: BALB/c * C57BL/6

behavior/neurological

abnormal liquid preference ( J:129526 )

• female, but not male, mice exhibit reduced preference for a natural reward in sucrose preference tests compared to wild-type mice

abnormal depression-related behavior ( J:129526 )

• female, but not male, mice exhibit more immobility when placed in swim test than wild-type mice

• unlike wild-type mice, female and male mice do not exhibit any decrease in immobility following administration of desipramine

increased locomotor activity ( J:129526 )

• male, but not female, mice exhibit increased locomotor activity compared to same sex wild-type mice

• however, activity levels are normal after 4 days of habituation