Phenotypes associated with this allele

• Allele Symbol: Pitx3^tm1Mli
• Allele Name: targeted mutation 1, Meng Li
• Allele ID: MGI:3573869
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pitx3^tm1Mli/Pitx3^tm1Mli	involves: 129P2/OlaHsd	MGI:3717001

Genotype
MGI:3717001

hm1

• Allelic Composition: Pitx3^tm1Mli/Pitx3^tm1Mli
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

increased neuron apoptosis ( J:99395 )

• number of apoptotic cells in SNc increases in mutant brains at E14.5 and in neonates compared to heterozygotes

• in newborn mice, there are fewer neurons in the SNc and many of these cells have pyknotic neurons

loss of dopaminergic neurons ( J:99395 )

• at E12.5, a 45-50% reduction in tyrosine hydroxylase-positive (TH) neurons compared to wild-type is observed in the midbrain

• at E14.5, loss of dopaminergic neurons (DA) is more severe in the substantia nigra pars compacta (SNc) than in the ventral tegmental area in mutants; number of TH-positive neurons in GFP-positive population of the SNc (53.4%) compared to heterozygotes (88.2%)

• numbers of TH-positive cells in the GFP-positive population is reduced at E12.5 (43.7%) compared to heterozygous controls (64.8%)

• there is a reduction in cells expressing GFP and TH in the adult midbrain; midbrain dopaminergic neurons are absent rather than defective

• loss of DA neurons is progressive starting at beginning of terminal differentiation into adulthood

increased neuron number ( J:99395 )

• at E14.5, there are more GFP-positive tyrosine hydroxylase-negative (TH-) neurons in homozygotes than in heterozygous controls

cellular

increased neuron apoptosis ( J:99395 )

• number of apoptotic cells in SNc increases in mutant brains at E14.5 and in neonates compared to heterozygotes

• in newborn mice, there are fewer neurons in the SNc and many of these cells have pyknotic neurons