All Phenotypes Rps6ka5<tm1Jsca> MGI Mouse

Phenotypes associated with this allele

Allelic Composition	Rps6ka5^tm1Jsca/Rps6ka5^tm1Jsca
Genetic Background	B6.Cg-Rps6ka5^tm1Jsca

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Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rps6ka5^tm1Jsca mutation (0 available); any Rps6ka5 mutation (64 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

impaired behavioral response to cocaine ( J:123042 )

• although mice experience a hyperlocomotor phase following initial exposure to cocaine no continued increase in locomotor activities is seen with repeated administration of the drug

enhanced conditioned place preference behavior ( J:123042 )

• mice are conditioned at lower doses (10mg/kg) of cocaine and after conditioning spent significantly more time in the cocaine-associated side

• mice showed no increased response at 20mg/kg

Allelic Composition	Rps6ka5^tm1Jsca/Rps6ka5^tm1Jsca
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rps6ka5^tm1Jsca mutation (0 available); any Rps6ka5 mutation (64 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

homeostasis/metabolism

increased susceptibility to neuronal excitotoxicity ( J:172062 )

• 3-NP, a mitochondrial neurotoxin, results in exacerbated striatal lesions, with increased neuronal loss, gliosis and nuclear fragmentation/compaction, compared to wild-type mice

nervous system

increased susceptibility to neuronal excitotoxicity ( J:172062 )

• 3-NP, a mitochondrial neurotoxin, results in exacerbated striatal lesions, with increased neuronal loss, gliosis and nuclear fragmentation/compaction, compared to wild-type mice

enlarged brain ventricles ( J:172062 )

• ventricular enlargement

cellular

increased susceptibility to neuronal excitotoxicity ( J:172062 )

• 3-NP, a mitochondrial neurotoxin, results in exacerbated striatal lesions, with increased neuronal loss, gliosis and nuclear fragmentation/compaction, compared to wild-type mice

Allelic Composition	Il10^tm1Cgn/Il10^tm1Cgn Rps6ka4^tm1Jsca/Rps6ka4^tm1Jsca Rps6ka5^tm1Jsca/Rps6ka5^tm1Jsca
Genetic Background	B6.Cg-Il10^tm1Cgn Rps6ka5^tm1Jsca Rps6ka4^tm1Jsca

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il10^tm1Cgn mutation (15 available); any Il10 mutation (45 available)
Rps6ka4^tm1Jsca mutation (0 available); any Rps6ka4 mutation (35 available)
Rps6ka5^tm1Jsca mutation (0 available); any Rps6ka5 mutation (64 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased interleukin-10 secretion ( J:139576 )

• there is no IL-10 secretion from bone marrow derived macrophages after LPS stimulation

increased interleukin-12a secretion ( J:139576 )

• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL12a than in controls during an 8 hour time period

increased interleukin-12b secretion ( J:139576 )

• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL12b than in controls during an 8 hour time period

increased interleukin-6 secretion ( J:139576 )

• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL-6 than in controls during an 8 hour time period

increased tumor necrosis factor secretion ( J:139576 )

• LPS stimulation of bone marrow derived macrophages leads to higher secretion of TNF than in controls during an 8 hour time period

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:139576 )

• less mice die 48 hours after sepsis induction by cecal ligation and puncture than in control

• 40% of mice die compared to 10% of wild-type mice subjected to the same sepsis model

immune system

abnormal cytokine level ( J:139576 )

decreased circulating interleukin-10 level ( J:139576 )

• LPS administration (1.8 mg/kg bodyweight) leads to significantly decreased levels of IL-10 four hours after administration compared to controls

• less IL-10 is also found in the plasma of mice undergoing sepsis from cecal ligation and puncture

increased circulating interleukin-12a level ( J:139576 )

• LPS administration (1.8 mg/kg bodyweight) leads to significantly increased levels of IL-12a four hours after administration compared to controls

increased circulating interleukin-12b level ( J:139576 )

• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL12b than in controls during an 8 hour time period

increased circulating interleukin-6 level ( J:139576 )

• LPS administration (1.8 mg/kg bodyweight) leads to significantly increased levels of IL-6 four hours after administration compared to controls

increased circulating tumor necrosis factor level ( J:139576 )

• LPS administration (1.8 mg/kg bodyweight) leads to significantly increased levels of TNF one hour after administration compared to controls

abnormal cytokine secretion ( J:139576 )

decreased interleukin-10 secretion ( J:139576 )

• LPS stimulation of bone marrow derived macrophages leads to decreased secretion of IL-10 than in controls during an 8 hour time period

• the discrepancy is greater during the early time points; 8 hours after stimulation secretion levels have reached 60% of wild-type

increased interleukin-12a secretion ( J:139576 )

• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL12a than in controls during an 8 hour time period

increased interleukin-12b secretion ( J:139576 )

• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL12b than in controls during an 8 hour time period

increased interleukin-6 secretion ( J:139576 )

• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL-6 than in controls during an 8 hour time period

increased tumor necrosis factor secretion ( J:139576 )

• stimulation of bone marrow derived macrophages leads to higher secretion of TNF than in controls during an 8 hour time period

increased susceptibility to endotoxin shock ( J:139576 )

• 2.5 mg/kg bodyweight causes LPS-induced toxicity in these mice while control mice are normal

• signs of toxicity include diarrhea, eye inflammation, piloerection, and a substantial drop in temperature within 4-8 hours of LPS injection

• mice were humanly sacrificed within 8 hours of LPS injection due to severity of symptoms

skin inflammation ( J:139576 )

• inflammation fails to resolve in phorbol 12-myristate 13-acetate (PMA) induced skin eczema of the ears

• ears of wild-type mice exposed to PMA swell from inflammatory infiltrate but resolve this swelling within 96 hours while mutant mice still have significant swelling 96 hours after PMA administration

• myeloperoxidase activity of ear extracts, a measure of polymorphonuclear cell recruitment, is significantly higher than controls both 72 and 96 hours after PMA administration

homeostasis/metabolism

abnormal cytokine level ( J:139576 )

decreased circulating interleukin-10 level ( J:139576 )

• LPS administration (1.8 mg/kg bodyweight) leads to significantly decreased levels of IL-10 four hours after administration compared to controls

• less IL-10 is also found in the plasma of mice undergoing sepsis from cecal ligation and puncture

increased circulating interleukin-12a level ( J:139576 )

• LPS administration (1.8 mg/kg bodyweight) leads to significantly increased levels of IL-12a four hours after administration compared to controls

increased circulating interleukin-12b level ( J:139576 )

• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL12b than in controls during an 8 hour time period

increased circulating interleukin-6 level ( J:139576 )

• LPS administration (1.8 mg/kg bodyweight) leads to significantly increased levels of IL-6 four hours after administration compared to controls

increased circulating tumor necrosis factor level ( J:139576 )

• LPS administration (1.8 mg/kg bodyweight) leads to significantly increased levels of TNF one hour after administration compared to controls

integument

skin inflammation ( J:139576 )

• inflammation fails to resolve in phorbol 12-myristate 13-acetate (PMA) induced skin eczema of the ears

• myeloperoxidase activity of ear extracts, a measure of polymorphonuclear cell recruitment, is significantly higher than controls both 72 and 96 hours after PMA administration

Allelic Composition	Rps6ka4^tm1Jsca/Rps6ka4^tm1Jsca Rps6ka5^tm1Jsca/Rps6ka5^tm1Jsca
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rps6ka4^tm1Jsca mutation (0 available); any Rps6ka4 mutation (35 available)
Rps6ka5^tm1Jsca mutation (0 available); any Rps6ka5 mutation (64 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased T cell proliferation ( J:124301 )

• T cells stimulated in vitro with anti-CD3 and anti-CD28 antibodies do not proliferate as well as wild-type cells

spleen hypoplasia ( J:124301 )

• at 6 weeks, mice have a reduction in the number of cells in the spleen

hematopoietic system

decreased T cell proliferation ( J:124301 )

• T cells stimulated in vitro with anti-CD3 and anti-CD28 antibodies do not proliferate as well as wild-type cells

spleen hypoplasia ( J:124301 )

• at 6 weeks, mice have a reduction in the number of cells in the spleen

cellular

decreased T cell proliferation ( J:124301 )

• T cells stimulated in vitro with anti-CD3 and anti-CD28 antibodies do not proliferate as well as wild-type cells

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