Analysis Tools
mortality/aging
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• frequently observed embryo resorption between E9.5 and E10.5
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cellular
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• the number of proliferating cells in E9.5 and E10.5 null embryos was reduced
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• establishment of proliferating MEFs from homozygous null embryos failed as cells were not capable of proliferating
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embryo
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• at E10.5, embryos failed to turn
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• the anterior-posterior axis of E10.5 embryos was markedly shortened
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• development of embryos was retarded with the E10.5 embryo head region resembling that of the E9 wildtype embryo and tissues caudal to the heart resembling the morphology of E8.5 or earlier wildtype embryos
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• reduced size of the deciduas and embryos between E8.5 and E10.5, however the layering and size of trophoblast giant cells at the proximal pole of the deciduas seemed normal
• positioning and size of the E9.5 null embryos resembled those of E7.5 wildtype embryos
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• E10.5 embryos had a neural tube closure defect with an overall structure comparable to that of the E8.5 wildtype embryo
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• impaired somitogenesis
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• primary vascular plexus formation in the yolk sac was delayed but blood vessel formation appeared unaffected
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growth/size/body
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• reduced size of the deciduas and embryos between E8.5 and E10.5, however the layering and size of trophoblast giant cells at the proximal pole of the deciduas seemed normal
• positioning and size of the E9.5 null embryos resembled those of E7.5 wildtype embryos
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nervous system
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• E10.5 embryos had a neural tube closure defect with an overall structure comparable to that of the E8.5 wildtype embryo
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