Phenotypes associated with this allele

• Allele Symbol: Ppard^tm1Wwah
• Allele Name: targeted mutation 1, Walter Wahli
• Allele ID: MGI:3575004
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ppard^tm1Wwah/Ppard^tm1Wwah	Not Specified	MGI:3575673
ht2	Ppard^tm1Wwah/Ppard^+	Not Specified	MGI:3575674

Genotype
MGI:3575673

hm1

• Allelic Composition: Ppard^tm1Wwah/Ppard^tm1Wwah
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, incomplete penetrance ( J:71272 )

• incomplete but high penetrance of lethality

integument

increased keratinocyte apoptosis ( J:96826 )

• increased apoptosis in developing hair follicles of skin at P4 but no difference in the proportion of apoptotic cells restricted to upper suprabasal layers of the interfollicular epidermis

decreased keratinocyte proliferation ( J:96826 )

• 10-20% decrease in the number of proliferative keratinocytes in early hair follicles at P4, but no difference in the interfollicular epidermis

thin hypodermis ( J:96826 )

• significant decrease in the thickness of the skin hypodermis from P4 onwards, resulting in reduced skin thickness

abnormal hair follicle development ( J:96826 )

• skin displays a reduced number of hair follicles at the advanced stages of morphogenesis and increased number of early hair germs and hair pegs, however total hair follicle number was normal

abnormal hair follicle peg morphology ( J:96826 )

• at P4, the number of early hair germs and hair pegs, characterized by the presence of mesenchyme at their base, is increased

underdeveloped hair follicles ( J:96826 )

• follicular growth is significantly delayed by P4 and P7, even though the first hair coat is indistinguishable from wild-type

thin skin ( J:96826 )

cellular

increased keratinocyte apoptosis ( J:96826 )

• increased apoptosis in developing hair follicles of skin at P4 but no difference in the proportion of apoptotic cells restricted to upper suprabasal layers of the interfollicular epidermis

decreased keratinocyte proliferation ( J:96826 )

• 10-20% decrease in the number of proliferative keratinocytes in early hair follicles at P4, but no difference in the interfollicular epidermis

Genotype
MGI:3575674

ht2

• Allelic Composition: Ppard^tm1Wwah/Ppard⁺
• Genetic Background: Not Specified

homeostasis/metabolism

delayed wound healing ( J:71272 )

• delay in wound healing throughout the whole repair process, with final closure postponed by 2-3 days, however skin elasticity was not impaired

• number of keratinocytes per surface unit is slightly higher at the edges of the skin wound

• cultured keratinocytes at the edges of a scrape wound detached more readily and exhibited a slower migration rate than wildtype

integument

abnormal keratinocyte adhesion ( J:71272 )

• isolated keratinoctyes are rounded and only adhered 4 days after seeding compared to controls that adhered and spread 24 hours after seeding

increased keratinocyte proliferation ( J:71272 )

• slight but significant increase in keratinocyte proliferation rate, however hair follicle cycle was normal

• increased keratinocyte proliferative response to tetradecanoylphorbol acetate (TPA) stimulation on the dorsal epidermis and in response to hair plucking of the dorsal skin

thick epidermis ( J:71272 )

• slight but significant increase in epidermal thickness, however no defect in epidermal differentiation

cellular

abnormal keratinocyte adhesion ( J:71272 )

• isolated keratinoctyes are rounded and only adhered 4 days after seeding compared to controls that adhered and spread 24 hours after seeding

increased keratinocyte proliferation ( J:71272 )

• slight but significant increase in keratinocyte proliferation rate, however hair follicle cycle was normal

• increased keratinocyte proliferative response to tetradecanoylphorbol acetate (TPA) stimulation on the dorsal epidermis and in response to hair plucking of the dorsal skin