Phenotypes associated with this allele

• Allele Symbol: Tg(Pomc1-cre)1Gsb
• Allele Name: transgene insertion 1, Gregory S Barsh
• Allele ID: MGI:3576071
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Camkk2^tm2.1Kpg/Camkk2^tm2.1Kpg Tg(CAG-Bgeo/GFP)21Lbe/0 Tg(Pomc1-cre)1Gsb/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:4946517
cn2	Prkaa2^tm1Vio/Prkaa2^tm1Vio Tg(Pomc1-cre)1Gsb/0	involves: 129S2/SvPas * FVB/N	MGI:3817314
cn3	Prkaa1^tm1Sbj/Prkaa1^tm1Sbj Prkaa2^tm1Vio/Prkaa2^tm1Vio Tg(Pomc1-cre)1Gsb/0	involves: 129S2/SvPas * FVB/N	MGI:3817316
cn4	Irs2^tm1With/Irs2^tm1With Tg(Pomc1-cre)1Gsb/0	involves: 129S6/SvEvTac * C57BL/6J	MGI:3576501
cn5	Atg5^tm1Myok/Atg5^tm1Myok Tg(Pomc1-cre)1Gsb/0	involves: 129S/SvEv * C57BL/6 * FVB/N	MGI:5784718
cn6	Atg12^tm1.1Jdth/Atg12^tm1.1Jdth Tg(Pomc1-cre)1Gsb/0	involves: C57BL/6 * FVB/N	MGI:5784715
cn7	Stk11^tm1Keis/Stk11^tm1Keis Tg(Pomc1-cre)1Gsb/0	involves: FVB/N	MGI:4946518

Genotype
MGI:4946517

cn1

• Allelic Composition: Camkk2^tm2.1Kpg/Camkk2^tm2.1Kpg; Tg(CAG-Bgeo/GFP)21Lbe/0; Tg(Pomc1-cre)1Gsb/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:170130 )

N • energy homeostasis and glucose handling are normal

Genotype
MGI:3817314

cn2

• Allelic Composition: Prkaa2^tm1Vio/Prkaa2^tm1Vio; Tg(Pomc1-cre)1Gsb/0
• Genetic Background: involves: 129S2/SvPas * FVB/N

homeostasis/metabolism

increased susceptibility to diet-induced obesity ( J:123951 )

• when fed a high fat diet, mice gain more weight than wild-type mice

abnormal metabolism ( J:123951 )

• mice exhibit reduced resting metabolic rate compared to wild-type mice

• however, daily energy expenditure is normal

growth/size/body

increased body weight ( J:123951 )

• at 11 weeks of age, mice weight 15% more than wild-type mice

increased susceptibility to diet-induced obesity ( J:123951 )

• when fed a high fat diet, mice gain more weight than wild-type mice

nervous system

nervous system phenotype ( J:123951 )

N • proopiomelanocortin neurons morphology and basal function are normal

abnormal neuron physiology ( J:123951 )

• proopiomelanocortin neurons do not respond electrophysically to glucose as do wild-type cells

• however, the electrophysical responses of proopiomelanocortin neuron to leptin and insulin are normal

adipose tissue

increased percent body fat/body weight ( J:123951 )

• mice exhibit increased adipocity and total body fat compared to wild-type mice

behavior/neurological

increased compensatory feeding amount ( J:123951 )

• mice exhibit increased compensatory feeding compared to wild-type mice after being fasted overnight

Genotype
MGI:3817316

cn3

• Allelic Composition: Prkaa1^tm1Sbj/Prkaa1^tm1Sbj; Prkaa2^tm1Vio/Prkaa2^tm1Vio; Tg(Pomc1-cre)1Gsb/0
• Genetic Background: involves: 129S2/SvPas * FVB/N

growth/size/body

increased body weight ( J:123951 )

• compared to wild-type mice

Genotype
MGI:3576501

cn4

• Allelic Composition: Irs2^tm1With/Irs2^tm1With; Tg(Pomc1-cre)1Gsb/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

normal phenotype

no abnormal phenotype detected ( J:97406 )

• fasting glucose levels, glucose tolerance, fasting insulin levels, pancreatic beta cell mass, body weight and length, body fat, food consumption, and leptin levels are normal

Genotype
MGI:5784718

cn5

• Allelic Composition: Atg5^tm1Myok/Atg5^tm1Myok; Tg(Pomc1-cre)1Gsb/0
• Genetic Background: involves: 129S/SvEv * C57BL/6 * FVB/N

cellular

abnormal autophagy ( J:232686 )

• complete loss of autophagy in hypothalamus neurons whether mice are fed a normal or high-fat diet

adipose tissue

adipose tissue phenotype ( J:232686 )

N • mice fed a normal diet exhibit normal fat mass and adiposity

growth/size/body

growth/size/body region phenotype ( J:232686 )

N • mice fed standard chow exhibit normal body weight, lean mass and body length

N • mice fed a high-fat diet exhibit normal body weight gain

nervous system

nervous system phenotype ( J:232686 )

N • mice exhibit normal POMC neuron numbers and corticotroph function

homeostasis/metabolism

abnormal autophagy ( J:232686 )

• complete loss of autophagy in hypothalamus neurons whether mice are fed a normal or high-fat diet

Genotype
MGI:5784715

cn6

• Allelic Composition: Atg12^tm1.1Jdth/Atg12^tm1.1Jdth; Tg(Pomc1-cre)1Gsb/0
• Genetic Background: involves: C57BL/6 * FVB/N

growth/size/body

growth/size/body region phenotype ( J:232686 )

N • mice fed standard chow exhibit normal body weight, lean mass and body length

N • mice fed a high-fat diet exhibit normal lean mass and body length

increased body fat mass ( J:232686 )

• in mice fed a high-fat diet

increased body weight ( J:232686 )

• in mice fed a high-fat diet

increased susceptibility to diet-induced obesity ( J:232686 )

• in mice fed a high-fat diet

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:232686 )

N • mice fed a high-fat diet exhibit normal insulin resistance, oxygen consumption and carbon dioxide elimination

abnormal autophagy ( J:232686 )

• complete loss of autophagy in hypothalamus neurons whether mice are fed a normal or high-fat diet

increased susceptibility to diet-induced obesity ( J:232686 )

• in mice fed a high-fat diet

impaired glucose tolerance ( J:232686 )

• in mice fed a high-fat diet

adipose tissue

adipose tissue phenotype ( J:232686 )

N • mice fed a normal diet exhibit normal fat mass and adiposity

increased total body fat amount ( J:232686 )

• in mice fed a high-fat diet

increased body fat mass ( J:232686 )

• in mice fed a high-fat diet

behavior/neurological

increased food intake ( J:232686 )

• in mice fed a high-fat diet

decreased locomotor activity ( J:232686 )

• in mice fed a high-fat diet during the dark cycle

cellular

abnormal autophagy ( J:232686 )

• complete loss of autophagy in hypothalamus neurons whether mice are fed a normal or high-fat diet

nervous system

nervous system phenotype ( J:232686 )

N • mice exhibit normal POMC neuron numbers and corticotroph function

skeleton

skeleton phenotype ( J:232686 )

N • mice fed a high-fat or normal diet exhibit normal bone mineral density

Genotype
MGI:4946518

cn7

• Allelic Composition: Stk11^tm1Keis/Stk11^tm1Keis; Tg(Pomc1-cre)1Gsb/0
• Genetic Background: involves: FVB/N

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:170130 )

N • mice exhibit normal energy expenditure, leptin concentration, and fasted plasma insulin levels

N • male mice exhibit normal glucose metabolism

abnormal gluconeogenesis ( J:170130 )

• hepatic glucose production induced by pyruvate or insulin is increased compared to in wild-type mice

impaired glucose tolerance ( J:170130 )

• in female mice, but not male mice

abnormal glycogen homeostasis ( J:170130 )

• female mice exhibit reduced whole-body glycogen synthesis compared with wild-type mice

insulin resistance ( J:170130 )

• after an insulin tolerance test, female mice exhibit reduced glucose clearance compared with wild-type mice

behavior/neurological

decreased fluid intake ( J:170130 )

• in mice treated with MT2

• however, unstimulated food intake is normal

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:170130 )

N • male and female mice exhibit normal pancreatic islet architecture, beta cell mass, and function

nervous system

nervous system phenotype ( J:170130 )

N • mice exhibit normal POMC neuron numbers compared with wild-type mice

N • POMC neurons exhibit normal glucose sensing