Phenotypes associated with this allele

• Allele Symbol: Irs2^tm1With
• Allele Name: targeted mutation 1, Dominic J Withers
• Allele ID: MGI:3576079
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Irs2^tm1With/Irs2^tm1With Tg(Nes-cre)1Kln/?	involves: 129S6/SvEvTac * C57BL/6 * SJL	MGI:3713798
cn2	Irs2^tm1With/Irs2^tm1With Tg(Ins2-cre)25Mgn/0	involves: 129S6/SvEvTac * C57BL/6J	MGI:3576499
cn3	Irs2^tm1With/Irs2^tm1With Tg(Nes-cre)1Kln/0	involves: 129S6/SvEvTac * C57BL/6J	MGI:3576500
cn4	Irs2^tm1With/Irs2^tm1With Tg(Pomc1-cre)1Gsb/0	involves: 129S6/SvEvTac * C57BL/6J	MGI:3576501

Genotype
MGI:3713798

cn1

• Allelic Composition: Irs2^tm1With/Irs2^tm1With; Tg(Nes-cre)1Kln/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

prolonged diestrus ( J:122064 )

♀ • mildly prolonged diestrus phase

prolonged estrous cycle ( J:122064 )

♀ • extended estrus cycle due to a mildly prolonged diestrus phase

♀ • however, female fertility and fecundity are normal

homeostasis/metabolism

abnormal prolactin level ( J:122064 )

• disestrus pituitary prolactin levels are reduced

Genotype
MGI:3576499

cn2

• Allelic Composition: Irs2^tm1With/Irs2^tm1With; Tg(Ins2-cre)25Mgn/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

homeostasis/metabolism

increased circulating glucose level ( J:97406 )

• fasting blood glucose is moderately increased at 12 weeks and 6 months of age but not increased 4 weeks of age; however mutants do not display overt diabetes

increased circulating insulin level ( J:97406 )

• fasting hyperinsulinemia is seen at 12 weeks and 6 months of age

increased circulating leptin level ( J:97406 )

• at 12 weeks, but not 5 weeks, of age leptin levels are significantly elevated

impaired glucose tolerance ( J:97406 )

• defective glucose disposal is seen at 12 weeks and 6 months of age

endocrine/exocrine glands

decreased pancreatic beta cell number ( J:97406 )

• by 12 weeks of age beta cell mass is reduced by 40% compared to control mice

• at 9 months of age islet area is increased compared to 12 week old mutants but decreased compared to controls

growth/size/body

increased body weight ( J:97406 )

• body weight is increased by 4 - 5 weeks of age and 20% more by 12 weeks of age compared to controls

increased body length ( J:97406 )

behavior/neurological

polyphagia ( J:97406 )

• at 5 and 12 weeks of age food consumption is increased

Genotype
MGI:3576500

cn3

• Allelic Composition: Irs2^tm1With/Irs2^tm1With; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

homeostasis/metabolism

increased circulating glucose level ( J:97406 )

• fasting blood glucose is moderately increased at 12 weeks and 6 months of age but not increased 4 weeks of age; however mutants do not display overt diabetes

increased circulating insulin level ( J:97406 )

• fasting hyperinsulinemia is seen at 12 weeks and 6 months of age

increased circulating leptin level ( J:97406 )

• at 12 weeks, but not 5 weeks, of age leptin levels are significantly elevated

impaired glucose tolerance ( J:97406 )

• mildly impaired glucose tolerance is seen at 12 weeks and 6 months of age

endocrine/exocrine glands

increased pancreatic beta cell mass ( J:97406 )

• by 12 weeks of age beta cell mass is increased compared to control mice

growth/size/body

increased body weight ( J:97406 )

• body weight is increased by 4 - 5 weeks of age and 20% more by 12 weeks of age compared to controls

increased body length ( J:97406 )

behavior/neurological

polyphagia ( J:97406 )

• at 5 and 12 weeks of age food consumption is increased

adipose tissue

increased total body fat amount ( J:97406 )

• at 16 weeks of age fat mass is significantly increased

Genotype
MGI:3576501

cn4

• Allelic Composition: Irs2^tm1With/Irs2^tm1With; Tg(Pomc1-cre)1Gsb/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

normal phenotype

no abnormal phenotype detected ( J:97406 )

• fasting glucose levels, glucose tolerance, fasting insulin levels, pancreatic beta cell mass, body weight and length, body fat, food consumption, and leptin levels are normal