Phenotypes associated with this allele

• Allele Symbol: Mbl2^tm1Kata
• Allele Name: targeted mutation 1, Kazue Takahashi
• Allele ID: MGI:3577339
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Mbl1^tm1Kata/Mbl1^tm1Kata Mbl2^tm1Kata/Mbl2^tm1Kata	involves: 129 * C57BL/6	MGI:3578158
cx2	Mbl1^tm1Kata/Mbl1^tm1Kata Mbl2^tm1Kata/Mbl2^tm1Kata	involves: 129 * C57BL/6J	MGI:3618387
cx3	Fcna^tm1Tefu/Fcna^tm1Tefu Mbl1^tm1Kata/Mbl1^tm1Kata Mbl2^tm1Kata/Mbl2^tm1Kata	involves: 129S4/SvJae	MGI:5297093

Genotype
MGI:3578158

cx1

• Allelic Composition: Mbl1^tm1Kata/Mbl1^tm1Kata; Mbl2^tm1Kata/Mbl2^tm1Kata
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

impaired macrophage phagocytosis ( J:97686 , J:98124 )

• demonstrated defective apoptotic cell clearance (16% of macrophages phagocytosed compared to more than 25% in wildtype), however did not develop spontaneous autoimmunity (J:97686)

• 38% decrease in percentage of phagocytic cells (J:97686)

• showed a 40% reduction in bacterial phagocytosis by resident peritoneal macrophages (J:98124)

abnormal B cell number ( J:97686 )

decreased germinal center B cell number ( J:97686 )

• more than 60% decrease in germinal center B cell numbers

increased B-1 B cell number ( J:97686 )

• increased number of peritoneal B1 cells, however no lymphoproliferation occurred

decreased macrophage cell number ( J:98124 )

• double homozygous mutants rendered neutropenic by cyclophosphamide (CY) injection had an 80% decrease in resident peritoneal macrophages

decreased monocyte cell number ( J:98124 )

• double homozygous mutants rendered neutropenic by cyclophosphamide (CY) injection had an 80% decrease in circulating monocytes

abnormal circulating interleukin-6 level ( J:98124 )

• serum IL-6 levels were reduced at 2 hours after S. aureus inoculation, however levels were increased 8-fold at 24 hours after inoculation compared to wildtype

abnormal circulating tumor necrosis factor level ( J:98124 )

• serum TNF-alpha levels were reduced at 2 hours after S. aureus inoculation, however levels were increased 15-fold at 24 hours after inoculation compared to wildtype

increased susceptibility to bacterial infection ( J:98124 )

• 100% of double mutants died 48 hours after S. aureus infection compared to 45% of wildtype

• statistically significant higher bacterial loads were found in the kidney, spleen, liver and blood compared with wildtype at 24 hours after S. aureus inoculation

• double homozygous mutants rendered neutropenic by cyclophosphamide (CY) injection exhibited increased susceptibility to bacterial infection with 21/29 developing abscesses in various organs compared to 3/15 of wildtype

hematopoietic system

impaired macrophage phagocytosis ( J:97686 , J:98124 )

• demonstrated defective apoptotic cell clearance (16% of macrophages phagocytosed compared to more than 25% in wildtype), however did not develop spontaneous autoimmunity (J:97686)

• 38% decrease in percentage of phagocytic cells (J:97686)

• showed a 40% reduction in bacterial phagocytosis by resident peritoneal macrophages (J:98124)

abnormal B cell number ( J:97686 )

decreased germinal center B cell number ( J:97686 )

• more than 60% decrease in germinal center B cell numbers

increased B-1 B cell number ( J:97686 )

• increased number of peritoneal B1 cells, however no lymphoproliferation occurred

decreased macrophage cell number ( J:98124 )

• double homozygous mutants rendered neutropenic by cyclophosphamide (CY) injection had an 80% decrease in resident peritoneal macrophages

decreased monocyte cell number ( J:98124 )

• double homozygous mutants rendered neutropenic by cyclophosphamide (CY) injection had an 80% decrease in circulating monocytes

homeostasis/metabolism

abnormal circulating interleukin-6 level ( J:98124 )

• serum IL-6 levels were reduced at 2 hours after S. aureus inoculation, however levels were increased 8-fold at 24 hours after inoculation compared to wildtype

abnormal circulating tumor necrosis factor level ( J:98124 )

• serum TNF-alpha levels were reduced at 2 hours after S. aureus inoculation, however levels were increased 15-fold at 24 hours after inoculation compared to wildtype

cellular

impaired macrophage phagocytosis ( J:97686 , J:98124 )

• demonstrated defective apoptotic cell clearance (16% of macrophages phagocytosed compared to more than 25% in wildtype), however did not develop spontaneous autoimmunity (J:97686)

• 38% decrease in percentage of phagocytic cells (J:97686)

• showed a 40% reduction in bacterial phagocytosis by resident peritoneal macrophages (J:98124)

Genotype
MGI:3618387

cx2

• Allelic Composition: Mbl1^tm1Kata/Mbl1^tm1Kata; Mbl2^tm1Kata/Mbl2^tm1Kata
• Genetic Background: involves: 129 * C57BL/6J

immune system

abnormal complement pathway ( J:106351 )

• after 45 min ischemia followed by 2 h reperfusion, complement activation as assessed by plasma C3a (desArg) level is significantly greater in wild-type mice than in double homozygous mutants; by 24 h, both groups have C3a levels < 30 ng/ml

abnormal response to infection ( J:106352 )

• doubly homozygous mutant mice infected intraperitoneally with herpes simplex virus type II (HSV-2) begin to clear the infection more slowly from the liver than do wild-type mice; however, complete clearance occurs in both by 6 days post-infection

• the proportion of HSV-2 neutralized in vitro after 9 minutes' exposure to serum from double homozygotes is significantly lower than after incubation with serum from wild-type mice; however, both have neutralized 90% of virus after > 21 minutes

increased susceptibility to bacterial infection ( J:106353 )

• all doubly homozygous mutant mice, but only 30% of wild-type mice, die within 42 h after burning of 5% of total body surface area (TBSA) followed by subcutaneous (s.c.) inoculation with Pseudomonas aeruginosa, whereas neither mutant nor wild-type mice appear ill following either treatment alone

• P. aeruginosa titers in plasma and homogenates of blood, liver and kidney harvested 20 h after the postburn inoculation are significantly higher in double homozygous than in wild-type mice; however, titers in lung and in skin do not differ significantly

renal/urinary system

decreased susceptibility to kidney reperfusion injury ( J:106351 )

• doubly homozygous mutant mice exhibit significantly less elevation of blood urea nitrogen (BUN) levels than do wild-type mice following induction of bilateral kidney eschemia (45 min) and reperfusion (24 h)

• after 45 min ischemia followed by 2 or 24 h reperfusion, histological examination of wild-type kidneys reveals severe tubular damage characterized by dilation, necrosis and presence of proteinaceous casts; kidneys of double homozygous mice exhibit no such damage

homeostasis/metabolism

decreased susceptibility to kidney reperfusion injury ( J:106351 )

• doubly homozygous mutant mice exhibit significantly less elevation of blood urea nitrogen (BUN) levels than do wild-type mice following induction of bilateral kidney eschemia (45 min) and reperfusion (24 h)

• after 45 min ischemia followed by 2 or 24 h reperfusion, histological examination of wild-type kidneys reveals severe tubular damage characterized by dilation, necrosis and presence of proteinaceous casts; kidneys of double homozygous mice exhibit no such damage

Genotype
MGI:5297093

cx3

• Allelic Composition: Fcna^tm1Tefu/Fcna^tm1Tefu; Mbl1^tm1Kata/Mbl1^tm1Kata; Mbl2^tm1Kata/Mbl2^tm1Kata
• Genetic Background: involves: 129S4/SvJae

immune system

immune system phenotype ( J:177350 )

N • mice exhibit normal collagen antibody-induced arthritis with normal complement alternative pathway activation