Phenotypes associated with this allele

• Allele Symbol: Spi1^tm1.2Nutt
• Allele Name: targeted mutation 1.2, Stephen L Nutt
• Allele ID: MGI:3578011
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Spi1^tm1.2Nutt/Spi1^tm1.2Nutt Tg(Mx1-cre)1Cgn/0	involves: C57BL/6 * CBA	MGI:3578821

Genotype
MGI:3578821

cn1

• Allelic Composition: Spi1^tm1.2Nutt/Spi1^tm1.2Nutt; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal granulocyte differentiation ( J:98188 )

• granulocytes maintained c-kit expression and displayed some, but not complete, maturation

extramedullary hematopoiesis ( J:98188 )

• spleen cells generated vastly increased numbers of granulocytic colonies, excessive numbers of megakaryocytic colonies and IL-3 responsive macrophage-containing colonies

abnormal bone marrow cell morphology/development ( J:98188 )

• the bone marrow contained normal total cellularity but exhibited an increased proportion of blast cells, and immature cells of the granulocyte lineage (promyelocytes and myelocytes)

absent common myeloid progenitor cells ( J:98188 )

• bone marrow lacked eosinophil colony-forming cells that were responsive to granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-3 or IL-5, but had greatly elevated numbers of granulocytic colonies developed when stimulated by G-CSF, IL-3 or stem cell factor but not with IL-6

• few or no megakaryoctye colonies developed in cultures of bone marrow cells under optimal culture for megakaryoctye formation

• loss of any readily identifiable lymphoid and myeloid progenitor or stem cell populations

absent erythrocytes ( J:98188 )

• bone marrow lacked the nucleated erythroid cells that represent 17% of the control bone marrow

increased granulocyte number ( J:98188 )

• expansion of granulocyte precursors in the bone marrow and spleen

decreased B cell number ( J:98188 )

• B lymphocyte numbers were reduced variably in the bone marrow, with most cells having a preB cell phenotype

abnormal spleen morphology ( J:98188 )

• in severe cases, spleen showed homogeneous infiltration by immature granulocytic forms and loss of splenic architecture

immune system

abnormal granulocyte differentiation ( J:98188 )

• granulocytes maintained c-kit expression and displayed some, but not complete, maturation

increased granulocyte number ( J:98188 )

• expansion of granulocyte precursors in the bone marrow and spleen

decreased B cell number ( J:98188 )

• B lymphocyte numbers were reduced variably in the bone marrow, with most cells having a preB cell phenotype

abnormal spleen morphology ( J:98188 )

• in severe cases, spleen showed homogeneous infiltration by immature granulocytic forms and loss of splenic architecture

cellular

abnormal granulocyte differentiation ( J:98188 )

• granulocytes maintained c-kit expression and displayed some, but not complete, maturation