Phenotypes associated with this allele

• Allele Symbol: Dicer1^tm1Mmk
• Allele Name: targeted mutation 1, Matthias Merkenschlager
• Allele ID: MGI:3578093
• Summary: 11 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Dicer1^tm1Mmk/Dicer1^tm1Mmk Fgf9^tm1.1Fwan/Fgf9^tm1.1Fwan Shh^tm1(EGFP/cre)Cjt/Shh^+	involves: 129 * 129S4/SvJaeSor * C57BL/6J	MGI:5751753
cn2	Dicer1^tm1Mmk/Dicer1^tm1Mmk Fgf9^tm1.1Fwan/Fgf9^+ Shh^tm1(EGFP/cre)Cjt/Shh^+	involves: 129 * 129S4/SvJaeSor * C57BL/6J	MGI:5751752
cn3	Dicer1^tm1Mmk/Dicer1^tm1Mmk Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0	involves: 129 * C57BL/6 * CD-1	MGI:4452405
cn4	Dicer1^tm1Mmk/Dicer1^tm1Mmk Tg(BGLAP-cre)1Clem/0	involves: 129 * C57BL/6 * FVB/N	MGI:4452406
cn5	Dicer1^tm1Mmk/Dicer1^tm1Mmk Shh^tm1(EGFP/cre)Cjt/Shh^+	involves: 129 * C57BL/6J	MGI:5751748
cn6	Bcl2l11^tm1.1Ast/Bcl2l11^tm1.1Ast Dicer1^tm1Mmk/Dicer1^tm1Mmk Cd79a^tm1(cre)Reth/Cd79a^+	involves: 129S1/Sv * BALB/c	MGI:3797396
cn7	Dicer1^tm1Mmk/Dicer1^tm1Mmk Tg(IghelMD4)4Ccg/0 Cd79a^tm1(cre)Reth/Cd79a^+	involves: BALB/c	MGI:3797394
cn8	Dicer1^tm1Mmk/Dicer1^tm1Mmk Cd79a^tm1(cre)Reth/Cd79a^+	involves: BALB/c	MGI:3797393
cn9	Dicer1^tm1Mmk/Dicer1^tm1.1Mmk Cd79a^tm1(cre)Reth/Cd79a^+	involves: BALB/c	MGI:3797392
cn10	Cd79a^tm1(cre)Reth/Cd79a^+ Dicer1^tm1Mmk/Dicer1^tm1Mmk Tg(BCL2)22Wehi/0	involves: BALB/c * C57BL/6JWehi * SJL/JWehi	MGI:3797395
cn11	Dicer1^tm1Mmk/Dicer1^tm1Mmk Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * DBA/2	MGI:3578520

Genotype
MGI:5751753

cn1

• Allelic Composition: Dicer1^tm1Mmk/Dicer1^tm1Mmk; Fgf9^tm1.1Fwan/Fgf9^tm1.1Fwan; Shh^{tm1(EGFP/cre)Cjt}/Shh⁺
• Genetic Background: involves: 129 * 129S4/SvJaeSor * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

respiratory system

respiratory system phenotype ( J:224791 )

N • mice show rescue of the lung phenotypes seen in single conditional Dicer1 mutants, showing smaller lungs with reduced cystic dilation of epithelial ducts

Genotype
MGI:5751752

cn2

• Allelic Composition: Dicer1^tm1Mmk/Dicer1^tm1Mmk; Fgf9^tm1.1Fwan/Fgf9⁺; Shh^{tm1(EGFP/cre)Cjt}/Shh⁺
• Genetic Background: involves: 129 * 129S4/SvJaeSor * C57BL/6J

respiratory system

respiratory system phenotype ( J:224791 )

N • mice show rescue of the lung phenotypes seen in single conditional Dicer1 mutants, showing smaller lungs, reduced epithelial dilation, mesenchymal thickness and mesenchymal proliferation

Genotype
MGI:4452405

cn3

• Allelic Composition: Dicer1^tm1Mmk/Dicer1^tm1Mmk; Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0
• Genetic Background: involves: 129 * C57BL/6 * CD-1

mortality/aging

decreased tumor-free survival time ( J:158901 )

• mice with tumors die by 4 to 5 weeks of age

• 30% of mice die by 8 weeks of age

craniofacial

increased facial tumor incidence ( J:158901 )

• at 2 to 3 weeks of age, mice develop facial tumors

hematopoietic system

hematopoietic system phenotype ( J:158901 )

N • mice exhibit normal frequency and function of hematopoietic stem cells and progenitors

enlarged spleen ( J:158901 )

• mice with myeloid sarcomas exhibit increased spleen weight with blast cell infiltration unlike in wild-type mice

extramedullary hematopoiesis ( J:158901 )

• in the spleen of some mice

anemia ( J:158901 )

• profound in some mice

impaired hematopoiesis ( J:158901 )

• hematopoietic progenitor cells exhibit increased apoptosis and proliferation compared with wild-type cells

• megakaryocyte-erythroid progenitor cells exhibit increased apoptosis compared to in wild-type mice

• wild-type mice transplanted with transgenic hematopoietic cells exhibit cytopenia and myelodysplastic features

• however, differentiated cells exhibit normal apoptosis

increased bone marrow cell number ( J:158901 )

• in some mice

abnormal megakaryocyte morphology ( J:158901 )

• micro-megakaryocytes with hypolobulated, hyperchromatic nuclei in the bone marrow

abnormal megakaryocyte differentiation ( J:158901 )

• impaired

thrombocytopenia ( J:158901 )

• profound in some mice

giant platelets ( J:158901 )

• giant platelets in the blood

decreased leukocyte cell number ( J:158901 )

• with all subsets of leukocytes exhibiting decreased numbers

decreased B cell number ( J:158901 )

• B cell and B cell progenitors are decreased in the bone marrow compared to in wild-type mice

abnormal myeloid leukocyte morphology ( J:158901 )

• myeloid cell frequency is increased in the bone marrow compared to in wild-type mice

abnormal granulocyte differentiation ( J:158901 )

abnormal neutrophil morphology ( J:158901 )

• hypersegementation

skeleton

abnormal bone structure ( J:158901 )

• texture of mineralized is altered compared to in wild-type mice

• however, trabecular and cortical bone volumes are normal

abnormal bone marrow morphology ( J:158901 )

• bone marrow vascularity is increased compared to in wild-type mice

decreased osteoblast cell number ( J:158901 )

• mildly

abnormal skeleton development ( J:158901 )

• mice exhibit reduced bone marrow stromal osteogenic colony numbers relative to total colony forming numbers compared with wild-type mice

• bone marrow stromal cultured exhibit reduced alkaline phosphatase and calcified matrix deposition compared with wild-type cultures

neoplasm

increased facial tumor incidence ( J:158901 )

• at 2 to 3 weeks of age, mice develop facial tumors

increased hemolymphoid system tumor incidence ( J:158901 )

• at 2 to 3 weeks of age, mice develop myeoblastic tumors

increased leukemia incidence ( J:158901 )

• mice exhibit sporadic acute myelogenous leukemia unlike wild-type mice

increased myeloid sarcoma incidence ( J:158901 )

• mice exhibit sporadic myeloid sarcoma with infiltration behavior unlike wild-type mice

cardiovascular system

abnormal cardiovascular system morphology ( J:158901 )

• bone marrow vascularity is increased compared to in wild-type mice

growth/size/body

increased facial tumor incidence ( J:158901 )

• at 2 to 3 weeks of age, mice develop facial tumors

postnatal growth retardation ( J:158901 )

enlarged spleen ( J:158901 )

• mice with myeloid sarcomas exhibit increased spleen weight with blast cell infiltration unlike in wild-type mice

immune system

enlarged spleen ( J:158901 )

• mice with myeloid sarcomas exhibit increased spleen weight with blast cell infiltration unlike in wild-type mice

decreased leukocyte cell number ( J:158901 )

• with all subsets of leukocytes exhibiting decreased numbers

decreased B cell number ( J:158901 )

• B cell and B cell progenitors are decreased in the bone marrow compared to in wild-type mice

abnormal myeloid leukocyte morphology ( J:158901 )

• myeloid cell frequency is increased in the bone marrow compared to in wild-type mice

abnormal granulocyte differentiation ( J:158901 )

abnormal neutrophil morphology ( J:158901 )

• hypersegementation

cellular

abnormal granulocyte differentiation ( J:158901 )

abnormal megakaryocyte differentiation ( J:158901 )

• impaired

Genotype
MGI:4452406

cn4

• Allelic Composition: Dicer1^tm1Mmk/Dicer1^tm1Mmk; Tg(BGLAP-cre)1Clem/0
• Genetic Background: involves: 129 * C57BL/6 * FVB/N

skeleton

abnormal bone structure ( J:158901 )

• texture of mineralized is altered compared to in wild-type mice

increased compact bone volume ( J:158901 )

• cortical bone volume is increased compared to in wild-type mice

hematopoietic system

hematopoietic system phenotype ( J:158901 )

N • mice exhibit normal hematopoietic morphology

Genotype
MGI:5751748

cn5

• Allelic Composition: Dicer1^tm1Mmk/Dicer1^tm1Mmk; Shh^{tm1(EGFP/cre)Cjt}/Shh⁺
• Genetic Background: involves: 129 * C57BL/6J

respiratory system

increased mesenchymal cell proliferation involved in lung development ( J:224791 )

• E12.5 lungs show increased mesenchymal proliferation

lung cyst ( J:224791 )

• E14.5 and E16.5 lungs show cystic expansion of the epithelial ducts

enlarged lung ( J:224791 )

• lungs are larger at E12.5 but are similar in size to controls at E14.5 and E16.5

abnormal branching involved in lung morphogenesis ( J:224791 )

• E12.5, E14.5, and E16.5 lungs exhibit reduced branching

abnormal lung-associated mesenchyme development ( J:224791 )

• E12.5 lungs exhibit expanded mesenchyme

abnormal lung epithelium morphology ( J:224791 )

• E12.5 lungs exhibit dilated epithelial ducts

• E14.5 and E16.5 lungs show cystic expansion of the epithelial ducts

cellular

increased mesenchymal cell proliferation involved in lung development ( J:224791 )

• E12.5 lungs show increased mesenchymal proliferation

growth/size/body

lung cyst ( J:224791 )

• E14.5 and E16.5 lungs show cystic expansion of the epithelial ducts

enlarged lung ( J:224791 )

• lungs are larger at E12.5 but are similar in size to controls at E14.5 and E16.5

Genotype
MGI:3797396

cn6

• Allelic Composition: Bcl2l11^tm1.1Ast/Bcl2l11^tm1.1Ast; Dicer1^tm1Mmk/Dicer1^tm1Mmk; Cd79a^tm1(cre)Reth/Cd79a⁺
• Genetic Background: involves: 129S1/Sv * BALB/c

immune system

decreased B cell number ( J:135783 )

• while B cell numbers are increased relative to in Cd79a^tm1(cre)Reth/Cd79a⁺ Bcl2l11^tm1.1Ast/Bcl2l11⁺ Dicer1^tm1Mmk/Dicer1^tm1Mmk mice, numbers are still lower than in wild-type mice

decreased mature B cell number ( J:135783 )

hematopoietic system

decreased B cell number ( J:135783 )

• while B cell numbers are increased relative to in Cd79a^tm1(cre)Reth/Cd79a⁺ Bcl2l11^tm1.1Ast/Bcl2l11⁺ Dicer1^tm1Mmk/Dicer1^tm1Mmk mice, numbers are still lower than in wild-type mice

decreased mature B cell number ( J:135783 )

Genotype
MGI:3797394

cn7

• Allelic Composition: Dicer1^tm1Mmk/Dicer1^tm1Mmk; Tg(IghelMD4)4Ccg/0; Cd79a^tm1(cre)Reth/Cd79a⁺
• Genetic Background: involves: BALB/c

immune system

decreased B cell number ( J:135783 )

• while B cell numbers are increased relative to in Cd79a^tm1(cre)Reth/Cd79a⁺ Dicer1^tm1Mmk/Dicer1^tm1Mmk mice, numbers are still lower than in wild-type mice

decreased mature B cell number ( J:135783 )

hematopoietic system

decreased B cell number ( J:135783 )

• while B cell numbers are increased relative to in Cd79a^tm1(cre)Reth/Cd79a⁺ Dicer1^tm1Mmk/Dicer1^tm1Mmk mice, numbers are still lower than in wild-type mice

decreased mature B cell number ( J:135783 )

Genotype
MGI:3797393

cn8

• Allelic Composition: Dicer1^tm1Mmk/Dicer1^tm1Mmk; Cd79a^tm1(cre)Reth/Cd79a⁺
• Genetic Background: involves: BALB/c

immune system

decreased immature B cell number ( J:135783 )

• hardly detected

increased pro-B cell number ( J:135783 )

• in the bone marrow

arrested B cell differentiation ( J:135783 )

• B cell development is almost completely blocked at the pro- to pre-B cell transition

decreased mature B cell number ( J:135783 )

• hardly detected

decreased pre-B cell number ( J:135783 )

• in the bone marrow due to a massive increase in apoptosis

hematopoietic system

decreased immature B cell number ( J:135783 )

• hardly detected

increased pro-B cell number ( J:135783 )

• in the bone marrow

arrested B cell differentiation ( J:135783 )

• B cell development is almost completely blocked at the pro- to pre-B cell transition

decreased mature B cell number ( J:135783 )

• hardly detected

decreased pre-B cell number ( J:135783 )

• in the bone marrow due to a massive increase in apoptosis

Genotype
MGI:3797392

cn9

• Allelic Composition: Dicer1^tm1Mmk/Dicer1^tm1.1Mmk; Cd79a^tm1(cre)Reth/Cd79a⁺
• Genetic Background: involves: BALB/c

immune system

abnormal immunoglobulin V(D)J recombination ( J:135783 )

• usage of Dh elements and N nucleotide insertion into Igkappa is increased compared to in wild-type mice

decreased B cell number ( J:135783 )

hematopoietic system

abnormal immunoglobulin V(D)J recombination ( J:135783 )

• usage of Dh elements and N nucleotide insertion into Igkappa is increased compared to in wild-type mice

decreased B cell number ( J:135783 )

Genotype
MGI:3797395

cn10

• Allelic Composition: Cd79a^tm1(cre)Reth/Cd79a⁺; Dicer1^tm1Mmk/Dicer1^tm1Mmk; Tg(BCL2)22Wehi/0
• Genetic Background: involves: BALB/c * C57BL/6JWehi * SJL/JWehi

immune system

decreased B cell number ( J:135783 )

• while B cell numbers are increased relative to in Cd79a^tm1(cre)Reth/Cd79a⁺ Dicer1^tm1Mmk/Dicer1^tm1Mmk mice, numbers are still lower than in wild-type mice

decreased mature B cell number ( J:135783 )

hematopoietic system

decreased B cell number ( J:135783 )

• while B cell numbers are increased relative to in Cd79a^tm1(cre)Reth/Cd79a⁺ Dicer1^tm1Mmk/Dicer1^tm1Mmk mice, numbers are still lower than in wild-type mice

decreased mature B cell number ( J:135783 )

Genotype
MGI:3578520

cn11

• Allelic Composition: Dicer1^tm1Mmk/Dicer1^tm1Mmk; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2

immune system

increased gamma-delta T cell number ( J:98192 )

• unusually high percentage of thymocytes expressed TCR gamma-delta and gamma-delta cells were prevalent in the double negative compartment and decreased numbers of alpha-beta T cells

decreased T cell number ( J:98192 )

• decreased numbers of alpha-beta T cells

decreased thymocyte number ( J:98192 )

• cell numbers in thymi were reduced nearly 10-fold relative to controls, however there were normal numbers of double negative cells in thym

• increased percentage (43% versus 11% of wildtype at 6 hours and 68% versus 30% at 24 hours) of dying thymocytes when grown in vitro

hematopoietic system

increased gamma-delta T cell number ( J:98192 )

• unusually high percentage of thymocytes expressed TCR gamma-delta and gamma-delta cells were prevalent in the double negative compartment and decreased numbers of alpha-beta T cells

decreased T cell number ( J:98192 )

• decreased numbers of alpha-beta T cells

decreased thymocyte number ( J:98192 )

• cell numbers in thymi were reduced nearly 10-fold relative to controls, however there were normal numbers of double negative cells in thym

• increased percentage (43% versus 11% of wildtype at 6 hours and 68% versus 30% at 24 hours) of dying thymocytes when grown in vitro

endocrine/exocrine glands

decreased thymocyte number ( J:98192 )

• cell numbers in thymi were reduced nearly 10-fold relative to controls, however there were normal numbers of double negative cells in thym

• increased percentage (43% versus 11% of wildtype at 6 hours and 68% versus 30% at 24 hours) of dying thymocytes when grown in vitro