Phenotypes associated with this allele

• Allele Symbol: Tg(HBB-cre)1Phi
• Allele Name: transgene insertion 1, Sjaak Philipsen
• Allele ID: MGI:3578635
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gata1^tm1Phi/Gata1^+ Tg(HBB-cre)1Phi/0 Tg(HBB-Gata1)G4Phi/0	involves: FVB	MGI:3579119
cn2	Gata1^tm1Phi/Gata1^+ Tg(HBB-cre)1Phi/0	involves: FVB	MGI:3579124

Genotype
MGI:3579119

cn1

• Allelic Composition: Gata1^tm1Phi/Gata1⁺; Tg(HBB-cre)1Phi/0; Tg(HBB-Gata1)G4Phi/0
• Genetic Background: involves: FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:98277 )

♀ • some mutant female embryos die by E13.5 - E14.0 and all are dead before birth similar to males hemizygous for Tg(HBB-Gata1)G4Phi

hematopoietic system

abnormal erythropoiesis ( J:98277 )

♀ • definitive erythropoiesis is impaired with at least 2-fold more primitive cells compared to definitive cells present at E14.0

♀ • compound mutant females are more severely affect compared to males hemizygous for Tg(HBB-Gata1)G4Phi and females heterozygous Gata1^tm1Phi and hemizygous for Tg(HBB-cre)1Phi

♀ • at E14.0, fewer cells beyond the polychromatic erythroblast stage are seen are seen in the liver and in hanging drop assays erythroid progenitors fail to differentiate

anemia ( J:98277 )

♀ • most surviving mutant female embryos are anemic at E13.5 - E14.0 similar to males hemizygous for Tg(HBB-Gata1)G4Phi

♀ • at E13.5, about 4% of the total circulating blood cells are immature erythroid precursors

Genotype
MGI:3579124

cn2

• Allelic Composition: Gata1^tm1Phi/Gata1⁺; Tg(HBB-cre)1Phi/0
• Genetic Background: involves: FVB

hematopoietic system

abnormal erythropoiesis ( J:98277 )

♀ • definitive erythropoiesis is impaired

♀ • at E14.0, fewer cells beyond the polychromatic erythroblast stage are seen are seen in the liver

♀ • differentiation of erythroid progenitors in the hanging drop assay is intermediate between wild-type mice and males hemizygous for Tg(HBB-Gata1)G4Phi

anemia ( J:98277 )

♀ • anemia is seen at E13.5 - E14.0; however, the rate of embryonic death is not increased compared to wild-type embryos

♀ • anemia is transient (not present at birth), variable, and not as severe as in hemizygous males; however at E13.5, about 4% of the total circulating blood cells are immature erythroid precursors