Phenotypes associated with this allele

• Allele Symbol: Nbn^tm2.1Zqw
• Allele Name: targeted mutation 2.1, Zhao-Qi Wang
• Allele ID: MGI:3578646
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nbn^tm2Zqw/Nbn^tm2.1Zqw Tg(Nes-cre)1Wme/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3579666

Genotype
MGI:3579666

cn1

• Allelic Composition: Nbn^tm2Zqw/Nbn^tm2.1Zqw; Tg(Nes-cre)1Wme/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal neuron differentiation ( J:98316 )

• neural stem and progenitor cells from E13.5 brains produce fewer and smaller neurospheres in culture, do not produce secondary neurospheres, and have an increased number of chromosomal breaks per metaphase; however, no increase in apoptosis is seen

• no primary neurospheres were isolated from newborn mutants

absent cerebellar foliation ( J:98316 )

abnormal cerebellum external granule cell layer morphology ( J:98316 )

• at E15.5, E18.5, and P7, proliferation of cells is decreased in the outer external granule cell layer

• at E15.5, E18.5, and P7, apoptosis is increased in the inner external granule cell layer

decreased brain weight ( J:98316 )

• seen at P7

abnormal cerebellar Purkinje cell layer ( J:98316 )

• at P0 the Purkinje cell layer is disorganized

abnormal cerebellar granule layer morphology ( J:98316 )

• at P0 the granule cell layer is disorganized with a reduction in the number of granule cells and ectopic presence of Purkinje cells

small cerebellum ( J:98316 )

• seen at P7-P21

abnormal astrocyte morphology ( J:98316 )

• at P0 the Bergmann glia are abnormal

behavior/neurological

abnormal reflex ( J:98316 )

• when suspended by the tail mutants stretch out their hind limbs

tremors ( J:98316 )

• seen after P7

ataxia ( J:98316 )

• cerebellar ataxia seen after P7

impaired balance ( J:98316 )

• after P7 mutants are unable to complete the balance beam test

akinesia ( J:98316 )

• seen after P7

abnormal gait ( J:98316 )

• seen after P7

increased stereotypic behavior ( J:98316 )

• increased repetitive movements seen after P7

growth/size/body

postnatal growth retardation ( J:98316 )

• detected by P7 and mutants reach only about half of the body weight of control mice at weaning

cellular

abnormal neuron differentiation ( J:98316 )

• neural stem and progenitor cells from E13.5 brains produce fewer and smaller neurospheres in culture, do not produce secondary neurospheres, and have an increased number of chromosomal breaks per metaphase; however, no increase in apoptosis is seen

• no primary neurospheres were isolated from newborn mutants