Phenotypes associated with this allele

• Allele Symbol: Plxna4^tm1Hfu
• Allele Name: targeted mutation 1, Hajime Fujisawa
• Allele ID: MGI:3578655
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Plxna4^tm1Hfu/Plxna4^tm1Hfu	B6.Cg-Plxna4^tm1Hfu	MGI:3579401
hm2	Plxna4^tm1Hfu/Plxna4^tm1Hfu	involves: C57BL/6 * CBA	MGI:3795749
ht3	Plxna4^tm1Hfu/Plxna4^+	B6.Cg-Plxna4^tm1Hfu	MGI:3579402
cx4	Plxna2^tm1Hfu/Plxna2^tm1Hfu Plxna4^tm1Hfu/Plxna4^tm1Hfu	involves: C57BL/6 * CBA	MGI:3795750

Genotype
MGI:3579401

hm1

• Allelic Composition: Plxna4^tm1Hfu/Plxna4^tm1Hfu
• Genetic Background: B6.Cg-Plxna4^tm1Hfu

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal axon guidance ( J:98330 )

• trajectories of the cranial and spinal nerves and projections of sympathetic ganglion axons were abnormal, however the position and configuration of cranial and spinal ganglia were normal

• defective pathfinding of fibers from the anterior olfactory nucleus to the anterior commissure

• growth cone collapse of dorsal root ganglion and sympathetic ganglion axons in response to Sema3A, but not Sema3F, was partially reduced and was completely abolished in sympathetic ganglion axons in response to Sema6A

abnormal sympathetic postganglionic fiber morphology ( J:98330 )

• aberrant projection of sympathetic ganglion axons at E12.5 and E15.5, with axons invading the anterior vertebrate muscles located just medial to sympathetic ganglia and connective tissues surrounding the vertebrae

abnormal anterior commissure morphology ( J:98330 )

• both acA (pars anterior) and acP (pars posterior) tracts were absent in the anterior commissure due to defective pathfinding of axons (fibers from the anterior olfactory nucleus never ran dorsally but shifted ventrally and then turned medially at the ventral surface of the forebrain and thus did not end up in the commissure)

abnormal anterior commissure pars anterior morphology ( J:98330 )

• absent

abnormal anterior commissure pars posterior morphology ( J:98330 )

• absent

disorganized barrel cortex ( J:98330 )

• the boundary of each barrel was indistinct, indicating defective formation of the barrels

abnormal sensory neuron innervation pattern ( J:98330 )

• defective trajectory and projection of peripheral sensory axons

abnormal facial nerve morphology ( J:98330 )

• distal parts of the facial nerve were disarrayed

abnormal glossopharyngeal nerve morphology ( J:98330 )

• distal parts of the glossopharyngeal nerve were disarrayed

abnormal trigeminal nerve morphology ( J:98330 )

• distal parts of the maxillary and mandibular nerves were disarrayed

• ophthalmic nerve fibers overshot and fanned out in their peripheral target fields

abnormal vagus nerve morphology ( J:98330 )

• distal parts of the vagus nerve were disarrayed

abnormal spinal nerve morphology ( J:98330 )

• pathways and projections of the spinal nerves were disorganized

• spinal nerve fibers within developing limbs were partially disarrayed and sometimes overshot

cellular

abnormal axon guidance ( J:98330 )

• trajectories of the cranial and spinal nerves and projections of sympathetic ganglion axons were abnormal, however the position and configuration of cranial and spinal ganglia were normal

• defective pathfinding of fibers from the anterior olfactory nucleus to the anterior commissure

• growth cone collapse of dorsal root ganglion and sympathetic ganglion axons in response to Sema3A, but not Sema3F, was partially reduced and was completely abolished in sympathetic ganglion axons in response to Sema6A

Genotype
MGI:3795749

hm2

• Allelic Composition: Plxna4^tm1Hfu/Plxna4^tm1Hfu
• Genetic Background: involves: C57BL/6 * CBA

nervous system

abnormal neuron differentiation ( J:135421 )

• exposure to recombinant SEMA6A protein fails to induce growth cone collapse of mossy fibers from cultured dentate gyrus fragments

abnormal hippocampal mossy fiber morphology ( J:135421 , J:160509 )

• mossy fibers invade most parts of CA3, particularly the stratum lacunosum-moleculare and the stratum oriens (J:135421)

• some fibers enter the suprapyramidal region (normal target) but are not as tightly packed as in wild-type mice (J:135421)

• in slice cocultures mossy fibers from the dentate gyrus massively invade the stratum lacunosum-moleculare and the stratum oriens and diffusely invade the stratum radiatum of either homozygous mutant or wild-type CA3 slices (J:135421)

• mossy fibers aberrantly spread into the stratum radiatum, stratum oriens, and stratum lacunosum-moleculare compared to in wild-type mice (J:160509)

• mossy fibers invade the inner and outer molecular layer of the dentate gyrus unlike in wild-type mice (J:160509)

cellular

abnormal neuron differentiation ( J:135421 )

• exposure to recombinant SEMA6A protein fails to induce growth cone collapse of mossy fibers from cultured dentate gyrus fragments

Genotype
MGI:3579402

ht3

• Allelic Composition: Plxna4^tm1Hfu/Plxna4⁺
• Genetic Background: B6.Cg-Plxna4^tm1Hfu

nervous system

abnormal axon guidance ( J:98330 )

• growth cone collapse of dorsal root ganglion and sympathetic ganglion axons in response to Sema3A, but not Sema3F, and sympathetic ganglion axons in response to Sema6A was partially reduced

abnormal anterior commissure morphology ( J:98330 )

abnormal anterior commissure pars anterior morphology ( J:98330 )

• thin acA (pars anterior) tract

abnormal anterior commissure pars posterior morphology ( J:98330 )

• extremely thin or missing acP (pars posterior) tract

abnormal cranial nerve morphology ( J:98330 )

• abnormal trajectories and projections of the cranial nerves that were less severe than in homozygotes

abnormal spinal nerve morphology ( J:98330 )

• abnormal trajectories and projections of the spinal nerves that were less severe than in homozgyotes

cellular

abnormal axon guidance ( J:98330 )

• growth cone collapse of dorsal root ganglion and sympathetic ganglion axons in response to Sema3A, but not Sema3F, and sympathetic ganglion axons in response to Sema6A was partially reduced

Genotype
MGI:3795750

cx4

• Allelic Composition: Plxna2^tm1Hfu/Plxna2^tm1Hfu; Plxna4^tm1Hfu/Plxna4^tm1Hfu
• Genetic Background: involves: C57BL/6 * CBA

nervous system

abnormal hippocampal mossy fiber morphology ( J:135421 )

• mossy fibers are widely distributed in CA3 as in mice homozygous for Plxna4^tm1Hfu alone