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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(Ins2-TFRC/OVA)296Wehi
transgene insertion 296, Walter and Eliza Hall Institute of Medical Research
MGI:3578729
Summary 9 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
 		C3tm1Crr/C3tm1Crr
Fcer1gtm1Rav/Fcer1gtm1Rav
Tg(Ins2-TFRC/OVA)296Wehi/0 		involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 MGI:3784426
cx2
 		Airetm1.1Doi/Airetm1.1Doi
Tg(Ins2-TFRC/OVA)296Wehi/0
Tg(TcraTcrb)425Cbn/0 		involves: 129P2/OlaHsd * C57BL/6 MGI:3803204
cx3
 		Fcer1gtm1Rav/Fcer1gtm1Rav
Tg(Ins2-TFRC/OVA)296Wehi/0 		involves: 129P2/OlaHsd * C57BL/6 MGI:3784425
cx4
 		Tg(Ins2-TFRC/OVA)296Wehi/0
Tg(TcraTcrb)425Cbn/0 		involves: 129P2/OlaHsd * C57BL/6 MGI:3803202
cx5
 		Airetm1Mand/Aire+
Tg(Ins2-TFRC/OVA)296Wehi/0
Tg(TcraTcrb)425Cbn/0 		involves: 129P2/OlaHsd * C57BL/6 MGI:3803203
cx6
 		C3tm1Crr/C3tm1Crr
Tg(Ins2-TFRC/OVA)296Wehi/0 		involves: 129S4/SvJae * C57BL/6 MGI:3784424
cx7
 		Fcgr2btm1Ttk/Fcgr2btm1Ttk
Tg(Ins2-TFRC/OVA)296Wehi/0 		involves: 129S4/SvJae * C57BL/6 MGI:3784429
cx8
 		Tg(Ins2-TFRC/OVA)296Wehi/0
1700016L21RikTg(Itgax-HBEGF/EGFP)57Lan/0 		involves: C57BL/6 * FVB MGI:3784427
tg9
 		Tg(Ins2-TFRC/OVA)296Wehi/0 involves: C57BL/6 MGI:3623748


Genotype
MGI:3784426
cx1
Allelic
Composition		 C3tm1Crr/C3tm1Crr
Fcer1gtm1Rav/Fcer1gtm1Rav
Tg(Ins2-TFRC/OVA)296Wehi/0
Genetic
Background		 involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
C3tm1Crr mutation (3 available); any C3 mutation (103 available)
Fcer1gtm1Rav mutation (8 available); any Fcer1g mutation (28 available)
Tg(Ins2-TFRC/OVA)296Wehi mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased susceptibility to autoimmune diabetes ( J:122114 )
• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG are completely protected from developing diabetes




Genotype
MGI:3803204
cx2
Allelic
Composition		 Airetm1.1Doi/Airetm1.1Doi
Tg(Ins2-TFRC/OVA)296Wehi/0
Tg(TcraTcrb)425Cbn/0
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Airetm1.1Doi mutation (2 available); any Aire mutation (52 available)
Tg(Ins2-TFRC/OVA)296Wehi mutation (1 available)
Tg(TcraTcrb)425Cbn mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal T cell differentiation ( J:135154 )
• negative selection of CD 4 T cells bearing the transgenic TCR is impaired as evidenced by only a slight reduction of clonotypic T cells occuring in the thymus and spleen
decreased CD4-positive, alpha-beta T cell number ( J:135154 )
• there is a slight reduction in the number of CD4 single positive thymocytes in the thymus compared to controls

hematopoietic system
abnormal T cell differentiation ( J:135154 )
• negative selection of CD 4 T cells bearing the transgenic TCR is impaired as evidenced by only a slight reduction of clonotypic T cells occuring in the thymus and spleen
decreased CD4-positive, alpha-beta T cell number ( J:135154 )
• there is a slight reduction in the number of CD4 single positive thymocytes in the thymus compared to controls




Genotype
MGI:3784425
cx3
Allelic
Composition		 Fcer1gtm1Rav/Fcer1gtm1Rav
Tg(Ins2-TFRC/OVA)296Wehi/0
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fcer1gtm1Rav mutation (8 available); any Fcer1g mutation (28 available)
Tg(Ins2-TFRC/OVA)296Wehi mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased T cell proliferation ( J:122114 )
• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in the presence of anti-ovalbumin IgG is abolished compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
decreased susceptibility to autoimmune diabetes ( J:122114 )
• only 40% of mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG exhibit diabetes compared to 100% of similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
• the severity of diabetes induced by Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG is reduced compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice

hematopoietic system
decreased T cell proliferation ( J:122114 )
• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in the presence of anti-ovalbumin IgG is abolished compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice

cellular
decreased T cell proliferation ( J:122114 )
• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in the presence of anti-ovalbumin IgG is abolished compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice




Genotype
MGI:3803202
cx4
Allelic
Composition		 Tg(Ins2-TFRC/OVA)296Wehi/0
Tg(TcraTcrb)425Cbn/0
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ins2-TFRC/OVA)296Wehi mutation (1 available)
Tg(TcraTcrb)425Cbn mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased double-positive T cell number ( J:135154 )
• the percentage of double-positive T cells in the thymus is increased by 20 % compared to control mice
decreased CD4-positive, alpha-beta T cell number ( J:135154 )
• there is a 10 fold drop in the number of CD4 single positive thymocytes in the thymus compared to controls due to increased negative selection of T cell bearing the transgenic TCR
• the number of CD4 T cells in the periphery that express the gene segments associated with the transgenic TCR is reduced almost by half compared to mice carrying just the Tg(TcraTcrb)425Cbn OT-II transgene

hematopoietic system
increased double-positive T cell number ( J:135154 )
• the percentage of double-positive T cells in the thymus is increased by 20 % compared to control mice
decreased CD4-positive, alpha-beta T cell number ( J:135154 )
• there is a 10 fold drop in the number of CD4 single positive thymocytes in the thymus compared to controls due to increased negative selection of T cell bearing the transgenic TCR
• the number of CD4 T cells in the periphery that express the gene segments associated with the transgenic TCR is reduced almost by half compared to mice carrying just the Tg(TcraTcrb)425Cbn OT-II transgene




Genotype
MGI:3803203
cx5
Allelic
Composition		 Airetm1Mand/Aire+
Tg(Ins2-TFRC/OVA)296Wehi/0
Tg(TcraTcrb)425Cbn/0
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Airetm1Mand mutation (0 available); any Aire mutation (52 available)
Tg(Ins2-TFRC/OVA)296Wehi mutation (1 available)
Tg(TcraTcrb)425Cbn mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal T cell differentiation ( J:135154 )
• negative selection fails to occur of CD 4 T cells bearing the transgenic TCR as evidenced by the normal number of clonotypic T cells found in the thymus and spleen

hematopoietic system
abnormal T cell differentiation ( J:135154 )
• negative selection fails to occur of CD 4 T cells bearing the transgenic TCR as evidenced by the normal number of clonotypic T cells found in the thymus and spleen




Genotype
MGI:3784424
cx6
Allelic
Composition		 C3tm1Crr/C3tm1Crr
Tg(Ins2-TFRC/OVA)296Wehi/0
Genetic
Background		 involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
C3tm1Crr mutation (3 available); any C3 mutation (103 available)
Tg(Ins2-TFRC/OVA)296Wehi mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
mortality/aging ( J:122114 )
N
• mice injected with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG survive unlike of similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice

immune system
increased T cell proliferation ( J:122114 )
• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in the presence or absence of anti-ovalbumin IgG is enhanced compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
insulitis ( J:122114 )
• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse alone exhibit higher a incidence of insulitis than of similarly treated Tg(Ins2-TFRC/OVA)296Wehi and Tg(Ins2-TFRC/OVA)296Wehi Fcer1gtm1Rav/Fcer1gtm1Rav mice
increased interferon-gamma secretion ( J:122114 )
• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse without anti-ovalbumin IgG exhibit an IFN-gamma production that is increased 3-fold compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
decreased susceptibility to autoimmune diabetes ( J:122114 )
• only 40% of mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG exhibit diabetes compared to 100% of similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
• the severity of diabetes induced by Tg(TcraTcrb)1100Mjb CD8+ T cells and anti-ovalbumin IgG is reduced compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice

endocrine/exocrine glands
insulitis ( J:122114 )
• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse alone exhibit higher a incidence of insulitis than of similarly treated Tg(Ins2-TFRC/OVA)296Wehi and Tg(Ins2-TFRC/OVA)296Wehi Fcer1gtm1Rav/Fcer1gtm1Rav mice

hematopoietic system
increased T cell proliferation ( J:122114 )
• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in the presence or absence of anti-ovalbumin IgG is enhanced compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice

cellular
increased T cell proliferation ( J:122114 )
• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in the presence or absence of anti-ovalbumin IgG is enhanced compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice




Genotype
MGI:3784429
cx7
Allelic
Composition		 Fcgr2btm1Ttk/Fcgr2btm1Ttk
Tg(Ins2-TFRC/OVA)296Wehi/0
Genetic
Background		 involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fcgr2btm1Ttk mutation (7 available); any Fcgr2b mutation (49 available)
Tg(Ins2-TFRC/OVA)296Wehi mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased susceptibility to autoimmune diabetes ( J:122114 )
• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG at 10-fold lower doses than in Tg(Ins2-TFRC/OVA)296Wehi mice develop diabetes




Genotype
MGI:3784427
cx8
Allelic
Composition		 Tg(Ins2-TFRC/OVA)296Wehi/0
1700016L21RikTg(Itgax-HBEGF/EGFP)57Lan/0
Genetic
Background		 involves: C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
1700016L21RikTg(Itgax-HBEGF/EGFP)57Lan mutation (6 available); any 1700016L21Rik mutation (4 available)
Tg(Ins2-TFRC/OVA)296Wehi mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased T cell proliferation ( J:122114 )
• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse is absent in pancreatic lymph nodes of dendritic cell depleted mice following treatment with diphtheria toxin regardless of whether mice receive anti-ovalbumin IgG
• however, transfer of dendritic cells into depleted mice restores proliferation

hematopoietic system
decreased T cell proliferation ( J:122114 )
• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse is absent in pancreatic lymph nodes of dendritic cell depleted mice following treatment with diphtheria toxin regardless of whether mice receive anti-ovalbumin IgG
• however, transfer of dendritic cells into depleted mice restores proliferation

cellular
decreased T cell proliferation ( J:122114 )
• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse is absent in pancreatic lymph nodes of dendritic cell depleted mice following treatment with diphtheria toxin regardless of whether mice receive anti-ovalbumin IgG
• however, transfer of dendritic cells into depleted mice restores proliferation




Genotype
MGI:3623748
tg9
Allelic
Composition		 Tg(Ins2-TFRC/OVA)296Wehi/0
Genetic
Background		 involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ins2-TFRC/OVA)296Wehi mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
increased susceptibility to induced morbidity/mortality ( J:122114 )
• 75% of mice injected with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG die by day 47

immune system
abnormal leukocyte migration ( J:98589 )
• injection of OVA-specific CD8+ T cell from OT-1 mice into transgenic female mice results in a 2- to 6-fold higher proportion of OT-1 CD8+ T cells/total CD8+ T cells in the kidney and pancreatic lymph nodes compared with mesenteric, inguinal or cervical lymph nodes or the spleen, while no accumulation of OT-1 CD8+ T cells is seen in non transgenic animals
abnormal CD8-positive, alpha beta T cell morphology ( J:98589 )
• 3 days following injection of OT-1 cells into transgenic animal, OVA-specific CD*+ T cells show activation and 50% of the cells have proliferated in a BrdU assay; injected non-transgenic animals show considerably less activation or proliferation
decreased interferon-gamma secretion ( J:122114 )
• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse without anti-ovalbumin IgG exhibit an IFN-gamma production that is decreased 3-fold compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi C3tm1Crr/C3tm1Crrmice
increased susceptibility to autoimmune diabetes ( J:122114 )
• mice injected with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG exhibit increased incidence of diabetes compared to mice receiving Tg(TcraTcrb)1100Mjb CD8+ T cells alone

hematopoietic system
abnormal leukocyte migration ( J:98589 )
• injection of OVA-specific CD8+ T cell from OT-1 mice into transgenic female mice results in a 2- to 6-fold higher proportion of OT-1 CD8+ T cells/total CD8+ T cells in the kidney and pancreatic lymph nodes compared with mesenteric, inguinal or cervical lymph nodes or the spleen, while no accumulation of OT-1 CD8+ T cells is seen in non transgenic animals
abnormal CD8-positive, alpha beta T cell morphology ( J:98589 )
• 3 days following injection of OT-1 cells into transgenic animal, OVA-specific CD*+ T cells show activation and 50% of the cells have proliferated in a BrdU assay; injected non-transgenic animals show considerably less activation or proliferation

cellular
abnormal leukocyte migration ( J:98589 )
• injection of OVA-specific CD8+ T cell from OT-1 mice into transgenic female mice results in a 2- to 6-fold higher proportion of OT-1 CD8+ T cells/total CD8+ T cells in the kidney and pancreatic lymph nodes compared with mesenteric, inguinal or cervical lymph nodes or the spleen, while no accumulation of OT-1 CD8+ T cells is seen in non transgenic animals




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory