Phenotypes associated with this allele

• Allele Symbol: Ptprc^tm1Tyb
• Allele Name: targeted mutation 1, Victor L J Tybulewicz
• Allele ID: MGI:3579087
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptprc^tm1Tyb/Ptprc^tm1Tyb	129S2/SvPas-Ptprc^tm1Tyb	MGI:3583681
hm2	Ptprc^tm1Tyb/Ptprc^tm1Tyb	B6.129S2-Ptprc^tm1Tyb	MGI:3583694
hm3	Ptprc^tm1Tyb/Ptprc^tm1Tyb	C.129S2-Ptprc^tm1Tyb	MGI:3583682
hm4	Ptprc^tm1Tyb/Ptprc^tm1Tyb	involves: 129S2/SvPas * C57BL/6 * DBA/2	MGI:3583680

Genotype
MGI:3583681

hm1

• Allelic Composition: Ptprc^tm1Tyb/Ptprc^tm1Tyb
• Genetic Background: 129S2/SvPas-Ptprc^tm1Tyb

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality at weaning, incomplete penetrance ( J:57681 )

• Background Sensitivity: display decreased viability around weaning, such that less than 25% survive to adulthood, that was not observed on a BALB/c or a mixed 129SvPas, C57BL/6, and DBA/2 background

digestive/alimentary system

diarrhea ( J:57681 )

• Background Sensitivity: severe diarrhea around weaning is seen on the 129S2/SvPas background but not on a BALB/c or a mixed 129SvPas, C57BL/6, and DBA/2 background

Genotype
MGI:3583694

hm2

• Allelic Composition: Ptprc^tm1Tyb/Ptprc^tm1Tyb
• Genetic Background: B6.129S2-Ptprc^tm1Tyb

immune system

increased NK cell number ( J:98190 )

• four- and fivefold increase in the percentage of NK1.1⁺CD49b⁺TCR-Beta^- NK cells in the spleen and liver, respectively, however percentage of NK precursors was normal

• increase in the turnover of NK cells in the spleen but not liver

abnormal NK cell physiology ( J:98190 )

• NK cells secrete less cytokines and chemokines when immunoreceptor tyrosine-based activation motif (ITAM)-containing surface receptors are stimulated

• no calcium flux in NK cells after Ly49D stimulation

abnormal cytokine secretion ( J:98190 )

• Ly49D-stimulated NK cells secrete less IFN-gamma

abnormal chemokine secretion ( J:98190 )

• Ly49D-stimulated NK cells produce 100-1000-fold less MIP-1alpha and MIP-1beta than controls

hematopoietic system

increased NK cell number ( J:98190 )

• four- and fivefold increase in the percentage of NK1.1⁺CD49b⁺TCR-Beta^- NK cells in the spleen and liver, respectively, however percentage of NK precursors was normal

• increase in the turnover of NK cells in the spleen but not liver

abnormal NK cell physiology ( J:98190 )

• NK cells secrete less cytokines and chemokines when immunoreceptor tyrosine-based activation motif (ITAM)-containing surface receptors are stimulated

• no calcium flux in NK cells after Ly49D stimulation

Genotype
MGI:3583682

hm3

• Allelic Composition: Ptprc^tm1Tyb/Ptprc^tm1Tyb
• Genetic Background: C.129S2-Ptprc^tm1Tyb

mortality/aging

mortality/aging ( J:57681 )

N • Background Sensitivity: a decrease in viability is not observed unlike in mice on a 129S2/SvPas background

immune system

immune system phenotype ( J:57681 )

N • Background Sensitivity: associated phenotypes appear on the BALB/c background but not on a 129S2/SvPas or a mixed 129SvPas, C57BL/6, and DBA/2 background

thymus hypoplasia ( J:57681 )

• thymic cellularity is 40% that of controls

enlarged spleen ( J:57681 )

• often have splenomegaly

abnormal T cell differentiation ( J:57681 )

• partial block at the DN3 (CD44^-CD25⁺) stage of development and a second block between double-positive and single-positive thymocytes

• less susceptible to single-positive cell depletion by staphylococcal enterotoxin B and endogenous Mls superantigens than controls

abnormal positive T cell selection ( J:57681 )

• 2.6-fold reduction, containing fewer TCR^hi or CD69⁺ cells, indicating failure of positive selection

increased double-negative T cell number ( J:57681 )

• 5-fold increase in the numbers of double negative CD44^-CD25⁺ cells

decreased double-positive T cell number ( J:57681 )

• 2.6-fold reduction, containing fewer TCR^hi or CD69⁺ cells, indicating failure of positive selection

decreased CD4-positive, alpha-beta T cell number ( J:57681 )

• 7-13-fold reduction in CD4+ T cells of the thymus, spleen and lymph nodes

decreased CD8-positive, alpha-beta T cell number ( J:57681 )

• 7-13-fold reduction in CD8+ T cells of the thymus, spleen and lymph nodes

abnormal T cell physiology ( J:57681 )

• calcium fluxes are greatly reduced in thymocytes

hematopoietic system

hematopoietic system phenotype ( J:57681 )

N • Background Sensitivity: associated phenotypes appear on the BALB/c background but not on a 129S2/SvPas or a mixed 129SvPas, C57BL/6, and DBA/2 background

thymus hypoplasia ( J:57681 )

• thymic cellularity is 40% that of controls

enlarged spleen ( J:57681 )

• often have splenomegaly

abnormal T cell differentiation ( J:57681 )

• partial block at the DN3 (CD44^-CD25⁺) stage of development and a second block between double-positive and single-positive thymocytes

• less susceptible to single-positive cell depletion by staphylococcal enterotoxin B and endogenous Mls superantigens than controls

abnormal positive T cell selection ( J:57681 )

• 2.6-fold reduction, containing fewer TCR^hi or CD69⁺ cells, indicating failure of positive selection

increased double-negative T cell number ( J:57681 )

• 5-fold increase in the numbers of double negative CD44^-CD25⁺ cells

decreased double-positive T cell number ( J:57681 )

• 2.6-fold reduction, containing fewer TCR^hi or CD69⁺ cells, indicating failure of positive selection

decreased CD4-positive, alpha-beta T cell number ( J:57681 )

• 7-13-fold reduction in CD4+ T cells of the thymus, spleen and lymph nodes

decreased CD8-positive, alpha-beta T cell number ( J:57681 )

• 7-13-fold reduction in CD8+ T cells of the thymus, spleen and lymph nodes

abnormal T cell physiology ( J:57681 )

• calcium fluxes are greatly reduced in thymocytes

endocrine/exocrine glands

thymus hypoplasia ( J:57681 )

• thymic cellularity is 40% that of controls

growth/size/body

enlarged spleen ( J:57681 )

• often have splenomegaly

Genotype
MGI:3583680

hm4

• Allelic Composition: Ptprc^tm1Tyb/Ptprc^tm1Tyb
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * DBA/2

normal phenotype

no abnormal phenotype detected ( J:57681 )

• Background Sensitivity: grossly normal and fertile, unlike on a 129S2/SvPas or BALB/c background