Phenotypes associated with this allele

• Allele Symbol: Tg(HBB-Gata1)G4Phi
• Allele Name: transgene insertion G4, Sjaak Philipsen
• Allele ID: MGI:3579105
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gata1^tm1Phi/Gata1^+ Tg(HBB-cre)1Phi/0 Tg(HBB-Gata1)G4Phi/0	involves: FVB	MGI:3579119
cx2	Gata1^tm1Phi/Gata1^+ Tg(HBB-Gata1)G4Phi/0	involves: FVB	MGI:3579126
tg3	Tg(HBB-Gata1)G4Phi/Y	involves: 129P2/OlaHsd * FVB	MGI:3579113
tg4	Tg(HBB-Gata1)G4Phi/Y	involves: FVB	MGI:3579122
tg5	Tg(HBB-Gata1)G4Phi/0	involves: 129P2/OlaHsd * FVB	MGI:3579114

Genotype
MGI:3579119

cn1

• Allelic Composition: Gata1^tm1Phi/Gata1⁺; Tg(HBB-cre)1Phi/0; Tg(HBB-Gata1)G4Phi/0
• Genetic Background: involves: FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:98277 )

♀ • some mutant female embryos die by E13.5 - E14.0 and all are dead before birth similar to males hemizygous for Tg(HBB-Gata1)G4Phi

hematopoietic system

abnormal erythropoiesis ( J:98277 )

♀ • definitive erythropoiesis is impaired with at least 2-fold more primitive cells compared to definitive cells present at E14.0

♀ • compound mutant females are more severely affect compared to males hemizygous for Tg(HBB-Gata1)G4Phi and females heterozygous Gata1^tm1Phi and hemizygous for Tg(HBB-cre)1Phi

♀ • at E14.0, fewer cells beyond the polychromatic erythroblast stage are seen are seen in the liver and in hanging drop assays erythroid progenitors fail to differentiate

anemia ( J:98277 )

♀ • most surviving mutant female embryos are anemic at E13.5 - E14.0 similar to males hemizygous for Tg(HBB-Gata1)G4Phi

♀ • at E13.5, about 4% of the total circulating blood cells are immature erythroid precursors

Genotype
MGI:3579126

cx2

• Allelic Composition: Gata1^tm1Phi/Gata1⁺; Tg(HBB-Gata1)G4Phi/0
• Genetic Background: involves: FVB

hematopoietic system

abnormal erythropoiesis ( J:98277 )

♀ • the onset of definitive erythropoiesis is delayed with a lower ratio of definitive to primitive erythroid cells at E13.5 and E14.0

♀ • differentiation of erythroid progenitors in the hanging drop assay is intermediate between wild-type mice and males hemizygous for Tg(HBB-Gata1)G4Phi

Genotype
MGI:3579113

tg3

• Allelic Composition: Tg(HBB-Gata1)G4Phi/Y
• Genetic Background: involves: 129P2/OlaHsd * FVB

mortality/aging

lethality throughout fetal growth and development, incomplete penetrance ( J:63773 )

♂ • hemizygous male embryos die by E14.5

hematopoietic system

abnormal erythropoiesis ( J:63773 )

♂ • at E13.5, the number of enucleated to nucleated erythrocytes is reduced more than 3-fold compared to wild-type males and more early basophilic erythoid precursors suggesting impairment of definitive erythropoiesis

anemia ( J:63773 )

♂ • hemizygous male embryos are anemic at E12.5

Genotype
MGI:3579122

tg4

• Allelic Composition: Tg(HBB-Gata1)G4Phi/Y
• Genetic Background: involves: FVB

mortality/aging

lethality throughout fetal growth and development, incomplete penetrance ( J:98277 )

♂ • some hemizygous male embryos die by E13.5 - E14.0 and all are dead before birth

hematopoietic system

abnormal erythropoiesis ( J:98277 )

♂ • definitive erythropoiesis is impaired

♂ • at E14.0, fewer erythroid cells in late in the differentiation process are seen in the liver and in hanging drop assays erythroid progenitors fail to differentiate

anemia ( J:98277 )

♂ • most surviving hemizygous male embryos are anemic at E13.5 - E14.0 with about 7-fold fewer cells in the blood

♂ • at E13.5, about 2% of the total circulating blood cells are immature erythroid precursors

Genotype
MGI:3579114

tg5

• Allelic Composition: Tg(HBB-Gata1)G4Phi/0
• Genetic Background: involves: 129P2/OlaHsd * FVB

normal phenotype

no abnormal phenotype detected ( J:63773 )

• hemizygous females are viable with normal definitive erythropoiesis