Phenotypes associated with this allele

• Allele Symbol: Cbfb^tm1Lhc
• Allele Name: targeted mutation 1, Lucio H Castilla
• Allele ID: MGI:3579250
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Cbfb^tm1Lhc/Cbfb^tm1Lhc Tg(Mx1-cre)1Cgn/0	involves: 129S6/SvEvTac * C57BL/6 * CBA	MGI:3615391
cn2	Cbfb^tm1Lhc/Cbfb^+ Tg(EIIa-cre)C5379Lmgd/0	involves: 129S6/SvEvTac * C57BL/6 * FVB/N	MGI:3615451

Genotype
MGI:3615391

cn1

• Allelic Composition: Cbfb^tm1Lhc/Cbfb^tm1Lhc; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal megakaryocyte differentiation ( J:105040 )

• there is a 14-fold decrease in mature megakaryocytes and a 2.3 fold reduction in immature megakaryocytes after treatment

abnormal myelopoiesis ( J:105040 )

• myeloid progenitors increase 3- to 9-fold over littermate controls after polyinosinic-polycytidylic injection (pIpC)

• there is a predominant population of abnormal myeloid progenitors

• abnormal progenitors have myeloid-erythroid progentior immunophenotype

• in culture, 1.5- to 2.5 fold increase is observed in erythroid and myeloid colonies generated from bone marrow

• number of smaller colonies compared to control is significantly greater

• proliferative capacity of abnormal myeloid precursors is reduced

anemia ( J:105040 )

thrombocytopenia ( J:105040 )

• platelet counts are reduced 50% at 2 weeks after injection with pIpC

increased leukocyte cell number ( J:105040 )

increased hematopoietic stem cell number ( J:105040 )

• numbers of hematopoietic stem cells increases 2-to 3-fold over littermate controls

abnormal spleen morphology ( J:105040 )

• infiltration of leukemic cells in spleen

enlarged spleen ( J:105040 )

• mice with acute myeloid leukemia have splenomegaly

liver/biliary system

abnormal liver morphology ( J:105040 )

• infiltration of leukemic cells in liver

immune system

abnormal myelopoiesis ( J:105040 )

• myeloid progenitors increase 3- to 9-fold over littermate controls after polyinosinic-polycytidylic injection (pIpC)

• there is a predominant population of abnormal myeloid progenitors

• abnormal progenitors have myeloid-erythroid progentior immunophenotype

• in culture, 1.5- to 2.5 fold increase is observed in erythroid and myeloid colonies generated from bone marrow

• number of smaller colonies compared to control is significantly greater

• proliferative capacity of abnormal myeloid precursors is reduced

increased leukocyte cell number ( J:105040 )

abnormal spleen morphology ( J:105040 )

• infiltration of leukemic cells in spleen

enlarged spleen ( J:105040 )

• mice with acute myeloid leukemia have splenomegaly

cellular

abnormal megakaryocyte differentiation ( J:105040 )

• there is a 14-fold decrease in mature megakaryocytes and a 2.3 fold reduction in immature megakaryocytes after treatment

growth/size/body

enlarged spleen ( J:105040 )

• mice with acute myeloid leukemia have splenomegaly

Genotype
MGI:3615451

cn2

• Allelic Composition: Cbfb^tm1Lhc/Cbfb⁺; Tg(EIIa-cre)C5379Lmgd/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * FVB/N

mortality/aging

embryonic lethality, complete penetrance ( J:105040 )

• embryos die by midgestation

hematopoietic system

impaired hematopoiesis ( J:105040 )

• embryos display hematopoeitic deficiency