Phenotypes associated with this allele

• Allele Symbol: Insr^tm1Shs
• Allele Name: targeted mutation 1, Takuji Shirasawa
• Allele ID: MGI:3580001
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Insr^tm1Shs/Insr^tm1Shs	involves: C57BL/6CrSlc	MGI:3580369
ht2	Insr^tm1Shs/Insr^+	involves: C57BL/6CrSlc	MGI:3580371

Genotype
MGI:3580369

hm1

• Allelic Composition: Insr^tm1Shs/Insr^tm1Shs
• Genetic Background: involves: C57BL/6CrSlc

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:98723 )

• homozygotes are born but die before 4 weeks of age from diabetic ketoacidosis

homeostasis/metabolism

ketoacidosis ( J:98723 )

• diabetic ketoacidosis

growth/size/body

postnatal growth retardation ( J:98723 )

Genotype
MGI:3580371

ht2

• Allelic Composition: Insr^tm1Shs/Insr⁺
• Genetic Background: involves: C57BL/6CrSlc

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:98723 )

• 4 month old heterozygous females and males survived 33.3% and 18.2% longer, respectively, when exposed to 80% oxygen compared to wild-type mice

• when exposed to 80% oxygen survival of ovariectomized heterozygous females is decreased and survival of estradiol (E2) treated heterozygous males is increased compared to untreated gender matched heterozygotes

• dietary restriction (65% of ad libitum) further increases the survival time of heterozygous males and females kept in 80% oxygen

decreased susceptibility to xenobiotic induced morbidity/mortality ( J:98723 )

• 4 month old heterozygous females and males began to die around 48 hours (similar to wild-type) after paraquat injection but survived longer overall compared to wild-type

homeostasis/metabolism

hyperglycemia ( J:98723 )

• seen in 6% of heterozygous males but not in females; however, no increase in urinary glucose is seen

increased circulating insulin level ( J:98723 )

• seen in males and females in the fed state

impaired glucose tolerance ( J:98723 )

• moderately impaired in heterozygous males but not in females

• estradiol (E2) treatment improved glucose tolerance in males

increased insulin sensitivity ( J:98723 )

• seen in heterozygous females

insulin resistance ( J:98723 )

• seen in heterozygous males

• estradiol (E2) treatment improved insulin sensitivity

decreased susceptibility to xenobiotic induced morbidity/mortality ( J:98723 )

• 4 month old heterozygous females and males began to die around 48 hours (similar to wild-type) after paraquat injection but survived longer overall compared to wild-type

adipose tissue

decreased total body fat amount ( J:98723 )

• fat mass is decreased by 41.3% and 57.5% in heterozygous females and males, respectively, compared to wild-type mice; however, no difference in food intake is seen

• when food intake is restricted to 65% of ad libitum, heterozygous females and males still have decreased fat mass compared to dietary restricted wild-type mice

liver/biliary system

abnormal liver physiology ( J:98723 )

• manganese superoxide dismutase activity is increased 39.9% and 22.9% in the livers of heterozygous females and males, respectively, compared to wild-type mice