Phenotypes associated with this allele

• Allele Symbol: Chrm1^tm1Kano
• Allele Name: targeted mutation 1, Masanobu Kano
• Allele ID: MGI:3580391
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Chrm1^tm1Kano/Chrm1^tm1Kano	involves: 129X1/SvJ	MGI:3616715
hm2	Chrm1^tm1Kano/Chrm1^tm1Kano	involves: 129X1/SvJ * C57BL/6	MGI:3581874
hm3	Chrm1^tm1Kano/Chrm1^tm1Kano	involves: 129X1/SvJ * C57BL/6J	MGI:4443055
cx4	Chrm1^tm1Kano/Chrm1^tm1Kano Chrm3^tm1Mmt/Chrm3^tm1Mmt	involves: 129X1/SvJ * C57BL/6	MGI:3581877
cx5	Chrm1^tm1Kano/Chrm1^tm1Kano Chrm5^tm1Minm/Chrm5^tm1Minm	involves: 129X1/SvJ * C57BL/6	MGI:3616717

Genotype
MGI:3616715

hm1

• Allelic Composition: Chrm1^tm1Kano/Chrm1^tm1Kano
• Genetic Background: involves: 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:105126 )

N • carbachol-induced intracellular calcium concentration increases in submandibular gland cells are indistinguishable from wild-type and homozygotes do not show frequent prandial drinking, indicating normal salivation

Genotype
MGI:3581874

hm2

• Allelic Composition: Chrm1^tm1Kano/Chrm1^tm1Kano
• Genetic Background: involves: 129X1/SvJ * C57BL/6

nervous system

abnormal inhibitory postsynaptic currents ( J:98902 )

• hippocampal neuron pairs exhibit a reduction in the enhanced depolarization-induced suppression of inhibitory (DSI) postsynaptic currents in response to the muscarinic agonist oxotremorine-M

Genotype
MGI:4443055

hm3

• Allelic Composition: Chrm1^tm1Kano/Chrm1^tm1Kano
• Genetic Background: involves: 129X1/SvJ * C57BL/6J

digestive/alimentary system

digestive/alimentary phenotype ( J:104782 )

N • normal intragastric pH, normal carbachol-, histamine-, or gastrin-stimulated gastric acid secretion, and normal serum gastrin and gastric histamine levels

Genotype
MGI:3581877

cx4

• Allelic Composition: Chrm1^tm1Kano/Chrm1^tm1Kano; Chrm3^tm1Mmt/Chrm3^tm1Mmt
• Genetic Background: involves: 129X1/SvJ * C57BL/6

nervous system

abnormal inhibitory postsynaptic currents ( J:98902 )

• hippocampal neuron pairs do not exhibit an enhanced depolarization-induced suppression of inhibitory (DSI) postsynaptic currents in response to the muscarinic agonist oxotremorine-M as was seen in controls

digestive/alimentary system

abnormal submandibular gland physiology ( J:105126 )

• carbachol-induced intracellular calcium concentration increase is completely absent in submandibular gland cells, however phenylephrine, an alpha1-adrenergic agonist, induces similar calcium responses as in wild-type

decreased salivation ( J:105126 )

• a high dose of pilocarpine induces almost no salivation compared to wild-type

endocrine/exocrine glands

abnormal submandibular gland physiology ( J:105126 )

• carbachol-induced intracellular calcium concentration increase is completely absent in submandibular gland cells, however phenylephrine, an alpha1-adrenergic agonist, induces similar calcium responses as in wild-type

decreased salivation ( J:105126 )

• a high dose of pilocarpine induces almost no salivation compared to wild-type

homeostasis/metabolism

decreased salivation ( J:105126 )

• a high dose of pilocarpine induces almost no salivation compared to wild-type

Genotype
MGI:3616717

cx5

• Allelic Composition: Chrm1^tm1Kano/Chrm1^tm1Kano; Chrm5^tm1Minm/Chrm5^tm1Minm
• Genetic Background: involves: 129X1/SvJ * C57BL/6

digestive/alimentary system

decreased digestive secretion ( J:104782 )

• decrease in gastric acid secretion in response to carbachol, with a significant decrease in cumulative acid output compared to wild type

• however gastric mucosal histology is normal

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:105126 )

N • no difference in pilocarpine-induced salivation from wild-type or in the frequency of prandial drinking, indicating normal salivation