Analysis Tools
mortality/aging
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• all embryos that survived to term died shortly after birth
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• progressive mortality with 4/39 embryos nonviable at E13.5, 4/15 embryos nonviable at E15.5, 4/19 embryos nonviable at E18.5 and the rest dying shortly after birth
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growth/size/body
craniofacial
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• 1/3 of embryos displayed craniofacial lesions
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nervous system
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• improper neural tube closure in some embryos
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exencephaly
(
J:99099
)
|
• observed in some embryos
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respiratory system
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• alveolar spaces in the lungs of dead E18.5 and P1 homozygous null mice were not inflated, indicating respiratory failure
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homeostasis/metabolism
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• majority of E18.5 embryos without exencephaly were initially alive but quickly became cyanotic
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cardiovascular system
liver/biliary system
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• number of cells recovered from E15.5 livers was about 50% that of wild-type and this was not due to a developmental arrest since the onset of hematopoiesis occurred at the correct time
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embryo
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• improper neural tube closure in some embryos
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cellular
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• MEFs exhibit decreased proliferative potential when cultured under oxidative stress and senesced prematurely
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• MEFs exhibit decreased proliferative potential when cultured under oxidative stress and senesced prematurely
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• MEFs from E13.5 embryos displayed increased sensitivity to oxidative-induced stress (as well as genotoxic and oncogene-induced stresses), showing lower cell densities throughout early passages and entering complete growth arrest earlier than controls when grown in 20% oxygen
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integument
