Phenotypes associated with this allele

• Allele Symbol: Fzd5^tm1Cle
• Allele Name: targeted mutation 1, Hans Clevers
• Allele ID: MGI:3583769
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Fzd5^tm1Cle/Fzd5^tm1Cle	involves: 129P2/OlaHsd	MGI:3716066
cn2	Fzd5^tm1Mmt/Fzd5^tm1Cle Tg(Six3-cre)69Frty/0	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * DBA/2	MGI:6379400
cn3	Fzd5^tm1Cle/Fzd5^tm1Cle Tg(Six3-cre)69Frty/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2	MGI:6379405

Genotype
MGI:3716066

cn1

• Allelic Composition: Fzd5^tm1Cle/Fzd5^tm1Cle
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

digestive/alimentary system

ectopic Paneth cells ( J:98680 )

• Paneth cells are randomly distributed in crypts and villi in mutants whereas they are localized to the base of crypts in wild-type mice

• highest number of mispositioned cells is found in the duodenum

• mispositioned cells contain no beta-catenin, and do not express cryptdin-1 mRNA, but contain lysozyme like normal Paneth cells

endocrine/exocrine glands

ectopic Paneth cells ( J:98680 )

• Paneth cells are randomly distributed in crypts and villi in mutants whereas they are localized to the base of crypts in wild-type mice

• highest number of mispositioned cells is found in the duodenum

• mispositioned cells contain no beta-catenin, and do not express cryptdin-1 mRNA, but contain lysozyme like normal Paneth cells

Genotype
MGI:6379400

cn2

• Allelic Composition: Fzd5^tm1Mmt/Fzd5^tm1Cle; Tg(Six3-cre)69Frty/0
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * DBA/2

vision/eye

vision/eye phenotype ( J:142601 )

N • mice exhibit normal optic fissure closure at E14.5

increased cornea thickness ( J:142601 )

• at E14.5

small lens ( J:142601 )

• variable and not highly significant reduction in diameter

microphthalmia ( J:142601 )

abnormal retina layer morphology ( J:142601 )

• abnormal lamination in some mice especially in the inner retinas and rosette structures

• however, retina distant from the hyperplastic vitreous vasculature is normal

disorganized retina ganglion layer ( J:142601 )

• adjacent to the hyperplastic vitreous vasculature mixed with neuroblast layer

increased total retina thickness ( J:142601 )

• at E14.5

retina detachment ( J:142601 )

• partial in some mice

• complete in some mice

retina fold ( J:142601 )

• retinal folds in some mice

vitreous body deposition ( J:142601 )

• retrolental mass at E12.5 and E17.5 that disrupts neuroblast lamination in the adjacent retinal area

abnormal eye physiology ( J:142601 )

• on going proliferation of hyperplastic vitreous vasculature

abnormal retina apoptosis ( J:142601 )

• increased apoptosis of ganglion cells adjacent to the hyperplastic vitreous vasculature, which fails to undergo apoptosis

cardiovascular system

abnormal vascular regression ( J:142601 )

• due to failure of vascular regression, the retrolental mass develops into pigmented hyperplastic primary vasculature that persists in postnatal stages (P0, P10 and P23) occasionally attached to the lens or the inner surface of the retina

cellular

abnormal vascular regression ( J:142601 )

• due to failure of vascular regression, the retrolental mass develops into pigmented hyperplastic primary vasculature that persists in postnatal stages (P0, P10 and P23) occasionally attached to the lens or the inner surface of the retina

abnormal retina apoptosis ( J:142601 )

• increased apoptosis of ganglion cells adjacent to the hyperplastic vitreous vasculature, which fails to undergo apoptosis

Genotype
MGI:6379405

cn3

• Allelic Composition: Fzd5^tm1Cle/Fzd5^tm1Cle; Tg(Six3-cre)69Frty/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * DBA/2

vision/eye

vision/eye phenotype ( J:136314 )

N • mice exhibit normal optic cup and lens development