Phenotypes associated with this allele

• Allele Symbol: Zfp830^tm1Cota
• Allele Name: targeted mutation 1, Michel Cohen-Tannoudji
• Allele ID: MGI:3583779
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Zfp830^tm1Cota/Zfp830^tm1Cota	either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6N)	MGI:3584105
ht2	Zfp830^tm1Cota/Zfp830^tm2.1Cota	involves: 129S2/SvPas * BALB/c * C57BL/6	MGI:4950718

Genotype
MGI:3584105

hm1

• Allelic Composition: Zfp830^tm1Cota/Zfp830^tm1Cota
• Genetic Background: either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6N)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality at implantation, complete penetrance ( J:99352 )

• homozygous embryos are present at E3.5 but fail to implant and are absent at E6.5

embryo

decreased inner cell mass proliferation ( J:99352 )

• in vivo at E4.5 the blastocyst contains about 30% fewer nuclei

absent inner cell mass proliferation ( J:99352 )

• outgrowth of the inner cell mass fails in culture

absent trophectoderm cell proliferation ( J:99352 )

• outgrowth of the trophectoderm fails in culture

failure of blastocyst to hatch from the zona pellucida ( J:99352 )

• most E3.5 embryos (16 of 23) fail to hatch from the zona pellucida in culture and most of those (5 of 7) that hatch fail to attach to the dish

abnormal inner cell mass morphology ( J:99352 )

• at E4.5 the inner cell mass is small, globular, and not closely apposed to the trophectoderm

abnormal trophectoderm morphology ( J:99352 )

• at E4.5 trophectoderm cells appear larger compared to control cells

cellular

abnormal cell cycle ( J:99352 )

• at E4.5 the number of mitotic cells is increased and abnormal mitotic figures are seen; however cells are still able to exit mitosis and the mitotic spindle checkpoint is functional

decreased inner cell mass proliferation ( J:99352 )

• in vivo at E4.5 the blastocyst contains about 30% fewer nuclei

absent inner cell mass proliferation ( J:99352 )

• outgrowth of the inner cell mass fails in culture

absent trophectoderm cell proliferation ( J:99352 )

• outgrowth of the trophectoderm fails in culture

reproductive system

failure of embryo implantation ( J:99352 )

♀

Genotype
MGI:4950718

ht2

• Allelic Composition: Zfp830^tm1Cota/Zfp830^tm2.1Cota
• Genetic Background: involves: 129S2/SvPas * BALB/c * C57BL/6

normal phenotype

no abnormal phenotype detected ( J:171647 )

• mice exhibit no phenotype