All Phenotypes Pick1<tm1Rlh> MGI Mouse

absent long-term depression ( J:107573 )

• cerebellar LTD is absent in Pick1-deficient mice as measured in dissociated cerebellar cultures and in parasagittal brain slices from P20-25 mice; transfection of cultured neurons with full length wild-type Pick1 cDNA rescues LTD

oligozoospermia ( J:149846 )

• total number of sperm from the cauda epididymis of adult homozygotes is significantly lower than that of wild-type and heterozygous controls

teratozoospermia ( J:149846 )

• ~90% of mutant sperm from the cauda epididymis are round-headed with abnormal acrosomes, abnormal nuclear shapes, and abnormal mitochondrial sheaths

abnormal sperm mitochondrial sheath morphology ( J:149846 )

• mutant mitochondrial sheaths exhibit various defects, including abnormal sperm with aggregated mitochondrial sheaths, split mitochondrial sheaths, mitochondrial sheaths overlapping with the round nucleus, and in some cases wrapping around the round nucleus

abnormal acrosome morphology ( J:149846 , J:233657 )

• mutant acrosomes fail to acquire the crescent moon-shaped structure, are frequently fragmented, and abnormally positioned (J:149846)

• fragmentation of acrosomes occurs in the early stages of spermiogenesis (J:149846)

• fragmented (J:233657)

globozoospermia ( J:149846 )

• ~90% of mutant sperm lack the typical hook-shaped head of wild-type sperm; instead, they have round or irregular ball-like heads

• globozoospermia is likely a consequence of abnormal trafficking of proacrosomal granules resulting in impaired formation of acrosomes

abnormal sperm nucleus morphology ( J:149846 , J:233657 )

• ~90% of mutant sperm from the cauda epididymis are round-headed with deformed (round) nuclei (J:149846)

• elongation of the nuclei of mutant spermatids is impaired in the late stages of spermiogenesis resulting in round-shaped nuclei (J:149846)

• impaired nucleus formation (J:233657)

increased male germ cell apoptosis ( J:149846 )

• TUNEL staining indicates significantly more apoptotic germ cells, many of them spermatids, in mutant seminiferous tubules than in wild-type tubules (2.5% vs 0.4%, respectively), suggesting increased apoptosis during late stages of spermatogenesis

asthenozoospermia ( J:149846 )

• the number of motile mutant sperm is less than 4% of that from wild-type littermates

• none of the mutant sperm display rapid progressive linear motility

small seminiferous tubules ( J:149846 )

• the diameter of homozygous mutant seminiferous tubules is significantly smaller than that of wild-type and heterozygous tubules

small testis ( J:149846 )

• homozygous mutant testes are smaller than those of wild-type and heterozygous mice

decreased testis weight ( J:149846 )

• normalized testis weights of homozygous mutant mice are significantly lower than those of both wild-type and heterozygous mice

abnormal spermiogenesis ( J:149846 )

• sperm abnormalities are likely occur in the transition from round spermatids to mature sperm during spermiogenesis

male infertility ( J:149846 )

• male homozygotes copulate normally but are completely infertile