Phenotypes associated with this allele

• Allele Symbol: Tg(HBA1-GFP)1Ew
• Allele Name: transgene insertion 1, Emma Whitelaw
• Allele ID: MGI:3586533
• Summary: 13 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Cdca7^em1Ldax/Cdca7^em1Ldax Tg(HBA1-GFP)1Ew/0	FVB/NJ-Cdca7^em1Ldax Tg(HBA1-GFP)1Ew	MGI:7614332
cx2	Foxo3^mommeR1/Foxo3^mommeR1 Tg(HBA1-GFP)1Ew/Tg(HBA1-GFP)1Ew	FVB/NJ-Foxo3^mommeR1 Tg(HBA1-Gfp)1Ew	MGI:4949205
cx3	Foxo3^mommeR1/Foxo3^tm1.1Rdp Tg(HBA1-GFP)1Ew/0	involves: 129S6/SvEvTac * C57BL/6 * FVB/NJ	MGI:4949206
cx4	Rif1^MommeD18/Rif1^+ Tg(HBA1-GFP)1Ew/Tg(HBA1-GFP)1Ew	involves: C57BL/6J * FVB/N	MGI:5581680
cx5	Tg(HBA1-GFP)1Ew/Tg(HBA1-GFP)1Ew Wiz^MommeD30/Wiz^MommeD30	involves: C57BL/6J * FVB/N	MGI:5581678
cx6	Tg(HBA1-GFP)1Ew/Tg(HBA1-GFP)1Ew Wiz^MommeD30/Wiz^+	involves: C57BL/6J * FVB/N	MGI:5581679
cx7	Eif3h^MommeD12/Eif3h^MommeD12 Tg(HBA1-GFP)1Ew/0	involves: C57BL/6J * FVB/NJ	MGI:5514389
cx8	Eif3h^MommeD38/Eif3h^+ Tg(HBA1-GFP)1Ew/0	involves: C57BL/6J * FVB/NJ	MGI:5514390
cx9	Eif3h^MommeD38/Eif3h^MommeD38 Tg(HBA1-GFP)1Ew/0	involves: C57BL/6J * FVB/NJ	MGI:5514391
cx10	Eif3h^MommeD12/Eif3h^MommeD38 Tg(HBA1-GFP)1Ew/0	involves: C57BL/6J * FVB/NJ	MGI:5514392
cx11	Eif3h^MommeD12/Eif3h^+ Tg(HBA1-GFP)1Ew/0	involves: C57BL/6J * FVB/NJ	MGI:5514388
cx12	Dnmt3b^MommeD14/Dnmt3b^MommeD14 Tg(HBA1-GFP)1Ew/0	involves: FVB/N	MGI:5515788
cx13	Dnmt3b^MommeD14/Dnmt3b^tm1Enl Tg(HBA1-GFP)1Ew/0	involves: FVB/N	MGI:5515792

Genotype
MGI:7614332

cx1

• Allelic Composition: Cdca7^em1Ldax/Cdca7^em1Ldax; Tg(HBA1-GFP)1Ew/0
• Genetic Background: FVB/NJ-Cdca7^em1Ldax Tg(HBA1-GFP)1Ew

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal DNA methylation ( J:345314 )

• lower levels of chromatin-bound CDCA7 protein in E10.5 embryos

• higher percentage of erythrocytes expressing Tg(HBA1-GFP)1Ew transgene at age P21

• lower transgene Tg(HBA1-GFP)1Ew DNA methylation levels at age P21

• global DNA hypomethylation in spleen, thymus, cerebrum and liver at age P21

• DNA hypomethylation of minor satellite repeats and intracisternal A particle (IAP) retrotransposons in spleen, thymus and liver at age P21

• normal DNA methylation of major satellite repeats in spleen, thymus and liver at age P21

embryo

embryo phenotype ( J:345314 )

N • mice born at expected Mendelian ratios

growth/size/body

growth/size/body region phenotype ( J:345314 )

N • no obvious phenotype

mortality/aging

mortality/aging ( J:345314 )

N • viable

N • mice present at expected Mendelian ratios at age P21

Genotype
MGI:4949205

cx2

• Allelic Composition: Foxo3^mommeR1/Foxo3^mommeR1; Tg(HBA1-GFP)1Ew/Tg(HBA1-GFP)1Ew
• Genetic Background: FVB/NJ-Foxo3^mommeR1 Tg(HBA1-Gfp)1Ew

cellular

abnormal polar body morphology ( J:170710 )

♀ • oocytes are less likely to have a polar body on ovulation

abnormal oogenesis ( J:170710 )

♀ • apparent asynchronous development of the oocyte and follicle

abnormal oocyte morphology ( J:170710 )

♀ • at P3 some primordial follicles show an increase in the size of primary oocytes

reproductive system

abnormal polar body morphology ( J:170710 )

♀ • oocytes are less likely to have a polar body on ovulation

abnormal oogenesis ( J:170710 )

♀ • apparent asynchronous development of the oocyte and follicle

abnormal oocyte morphology ( J:170710 )

♀ • at P3 some primordial follicles show an increase in the size of primary oocytes

absent corpus luteum ( J:170710 )

♀ • at 18 weeks of age

increased corpora lutea number ( J:170710 )

♀ • at P30 compared to heterozygous controls

abnormal ovarian follicle morphology ( J:170710 )

♀ • in some ovaries a disproportionate increase in oocyte size relative to the follicular cells is seen at P30

abnormal ovarian follicle number ( J:170710 )

♀ • increase in the number of follicles at P30

decreased primary ovarian follicle number ( J:170710 )

♀ • marked decrease in the number of primary follicles at P60

increased secondary ovarian follicle number ( J:170710 )

♀ • increase in the number of secondary follicles at P30

absent ovarian follicles ( J:170710 )

♀ • at 18 weeks of age ovarian follicles are absent in 3 of 4 mice and the remaining mouse has a few degenerating primordial follicles

abnormal primordial ovarian follicle morphology ( J:170710 )

♀ • at P3 some primordial follicles show an increase in the size of primary oocytes

♀ • at P14 most of the remaining primordial follicles contain an enlarged primary oocyte surrounded by spindle-shaped/squamous follicular cells

decreased primordial ovarian follicle number ( J:170710 )

♀ • marked decrease in the number of primordial follicles at P60

♀ • at 18 weeks of age 1 of 4 mice has a few degenerating primordial follicles

enlarged ovary ( J:170710 )

♀ • at P30 compared to heterozygous controls

♀ • at 1 year of age occasional females have grossly enlarged ovaries

increased ovarian teratoma incidence ( J:170710 )

♀ • found in grossly enlarged ovaries at 1 year of age

♀ • Background Sensitivity: teratomas are seen in about 1 in 6 mice of aged up to 8 months in mice on an FVB/NJ background but not in mice on an inbred C57BL/6J background

abnormal ovary physiology ( J:170710 )

♀ • apparent asynchronous development of the oocyte and follicle

early reproductive senescence ( J:170710 )

• females lose fertility by about 8 weeks of age

• however, males display normal fertility

abnormal superovulation ( J:170710 )

♀ • oocytes from superovulated females are less likely to have a polar body on the day of ovulation

♀ • increased proportion of superovulated oocytes that are arrested at metaphase of meiosis I

♀ • oocytes cultured overnight are more likely to fragment than those from superovulated heterozygous females

neoplasm

increased ovarian teratoma incidence ( J:170710 )

♀ • found in grossly enlarged ovaries at 1 year of age

♀ • Background Sensitivity: teratomas are seen in about 1 in 6 mice of aged up to 8 months in mice on an FVB/NJ background but not in mice on an inbred C57BL/6J background

growth/size/body

enlarged ovary ( J:170710 )

♀ • at P30 compared to heterozygous controls

♀ • at 1 year of age occasional females have grossly enlarged ovaries

mortality/aging

early reproductive senescence ( J:170710 )

• females lose fertility by about 8 weeks of age

• however, males display normal fertility

endocrine/exocrine glands

absent corpus luteum ( J:170710 )

♀ • at 18 weeks of age

increased corpora lutea number ( J:170710 )

♀ • at P30 compared to heterozygous controls

abnormal ovarian follicle morphology ( J:170710 )

♀ • in some ovaries a disproportionate increase in oocyte size relative to the follicular cells is seen at P30

abnormal ovarian follicle number ( J:170710 )

♀ • increase in the number of follicles at P30

decreased primary ovarian follicle number ( J:170710 )

♀ • marked decrease in the number of primary follicles at P60

increased secondary ovarian follicle number ( J:170710 )

♀ • increase in the number of secondary follicles at P30

absent ovarian follicles ( J:170710 )

♀ • at 18 weeks of age ovarian follicles are absent in 3 of 4 mice and the remaining mouse has a few degenerating primordial follicles

abnormal primordial ovarian follicle morphology ( J:170710 )

♀ • at P3 some primordial follicles show an increase in the size of primary oocytes

♀ • at P14 most of the remaining primordial follicles contain an enlarged primary oocyte surrounded by spindle-shaped/squamous follicular cells

decreased primordial ovarian follicle number ( J:170710 )

♀ • marked decrease in the number of primordial follicles at P60

♀ • at 18 weeks of age 1 of 4 mice has a few degenerating primordial follicles

enlarged ovary ( J:170710 )

♀ • at P30 compared to heterozygous controls

♀ • at 1 year of age occasional females have grossly enlarged ovaries

increased ovarian teratoma incidence ( J:170710 )

♀ • found in grossly enlarged ovaries at 1 year of age

♀ • Background Sensitivity: teratomas are seen in about 1 in 6 mice of aged up to 8 months in mice on an FVB/NJ background but not in mice on an inbred C57BL/6J background

abnormal ovary physiology ( J:170710 )

♀ • apparent asynchronous development of the oocyte and follicle

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
dermoid cyst of ovary		DOID:5117	OMIM:166950	J:170710

Genotype
MGI:4949206

cx3

• Allelic Composition: Foxo3^mommeR1/Foxo3^tm1.1Rdp; Tg(HBA1-GFP)1Ew/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * FVB/NJ

reproductive system

decreased primary ovarian follicle number ( J:170710 )

♀ • marked reduction in the number of primary follicles in some mice at 12 weeks of age

decreased primordial ovarian follicle number ( J:170710 )

♀ • marked reduction in the number of primordial follicles in some mice at 12 weeks of age

absent ovarian follicles ( J:170710 )

♀ • in some mice at 12 weeks of age

neoplasm

neoplasm ( J:170710 )

N • unlike mice homozygous for Foxo3^mommeR1 no ovarian teratomas are detected (in 7 mice)

endocrine/exocrine glands

decreased primary ovarian follicle number ( J:170710 )

♀ • marked reduction in the number of primary follicles in some mice at 12 weeks of age

decreased primordial ovarian follicle number ( J:170710 )

♀ • marked reduction in the number of primordial follicles in some mice at 12 weeks of age

absent ovarian follicles ( J:170710 )

♀ • in some mice at 12 weeks of age

Genotype
MGI:5581680

cx4

• Allelic Composition: Rif1^MommeD18/Rif1⁺; Tg(HBA1-GFP)1Ew/Tg(HBA1-GFP)1Ew
• Genetic Background: involves: C57BL/6J * FVB/N

cellular

abnormal epigenetic regulation of gene expression ( J:201508 )

Genotype
MGI:5581678

cx5

• Allelic Composition: Tg(HBA1-GFP)1Ew/Tg(HBA1-GFP)1Ew; Wiz^MommeD30/Wiz^MommeD30
• Genetic Background: involves: C57BL/6J * FVB/N

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:201508 )

Genotype
MGI:5581679

cx6

• Allelic Composition: Tg(HBA1-GFP)1Ew/Tg(HBA1-GFP)1Ew; Wiz^MommeD30/Wiz⁺
• Genetic Background: involves: C57BL/6J * FVB/N

cellular

abnormal epigenetic regulation of gene expression ( J:201508 )

Genotype
MGI:5514389

cx7

• Allelic Composition: Eif3h^MommeD12/Eif3h^MommeD12; Tg(HBA1-GFP)1Ew/0
• Genetic Background: involves: C57BL/6J * FVB/NJ

mortality/aging

embryonic lethality, complete penetrance ( J:201509 )

• only empty deciduas are present at E9.5

reproductive system

empty decidua capsularis ( J:201509 )

• at E9.5

Genotype
MGI:5514390

cx8

• Allelic Composition: Eif3h^MommeD38/Eif3h⁺; Tg(HBA1-GFP)1Ew/0
• Genetic Background: involves: C57BL/6J * FVB/NJ

cellular

abnormal epigenetic regulation of gene expression ( J:201509 )

Genotype
MGI:5514391

cx9

• Allelic Composition: Eif3h^MommeD38/Eif3h^MommeD38; Tg(HBA1-GFP)1Ew/0
• Genetic Background: involves: C57BL/6J * FVB/NJ

mortality/aging

embryonic lethality, complete penetrance ( J:201509 )

• only empty deciduas are present at E9.5

reproductive system

empty decidua capsularis ( J:201509 )

• at E9.5

Genotype
MGI:5514392

cx10

• Allelic Composition: Eif3h^MommeD12/Eif3h^MommeD38; Tg(HBA1-GFP)1Ew/0
• Genetic Background: involves: C57BL/6J * FVB/NJ

mortality/aging

preweaning lethality, complete penetrance ( J:201509 )

• no mice are present at weaning

Genotype
MGI:5514388

cx11

• Allelic Composition: Eif3h^MommeD12/Eif3h⁺; Tg(HBA1-GFP)1Ew/0
• Genetic Background: involves: C57BL/6J * FVB/NJ

cellular

abnormal epigenetic regulation of gene expression ( J:201509 )

Genotype
MGI:5515788

cx12

• Allelic Composition: Dnmt3b^MommeD14/Dnmt3b^MommeD14; Tg(HBA1-GFP)1Ew/0
• Genetic Background: involves: FVB/N

cellular

abnormal epigenetic regulation of gene expression ( J:198944 )

• surviving homozygous mice displayed a higher percentage of GFP-expressing cells than did the heterozygotes

• significantly reduced methylation of transgene DNA in homozygous individuals (28 % of CpGs methylated) compared with wild type littermates (62 % of CpGs methylated), consistent with the change in transcriptional activity

mortality/aging

preweaning lethality, incomplete penetrance ( J:198944 )

• heterozygous intercrosses only resulted in 10% of homozygous offspring instead of the expected 25%

Genotype
MGI:5515792

cx13

• Allelic Composition: Dnmt3b^MommeD14/Dnmt3b^tm1Enl; Tg(HBA1-GFP)1Ew/0
• Genetic Background: involves: FVB/N

cellular

abnormal epigenetic regulation of gene expression ( J:198944 )

• compound heterozygous mice display a higher percentage of GFP-expressing cells than heterozygotes

• significantly reduced methylation of transgene DNA in homozygous individuals (28 % of CpGs methylated) compared with wild type littermates (62 % of CpGs methylated), consistent with the change in transcriptional activity

growth/size/body

abnormal head morphology ( J:198944 )

• hypotelorism and other cranial defects are seen, similar to phenotypes of other alleles of this gene

decreased body height ( J:198944 )

• small in stature