All Phenotypes Tg(TcraCl4,TcrbCl4)1Shrm MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(HA)Libl/Gt(ROSA)26Sor^tm1(HA)Libl Mog^tm1(cre)Gkl/? Tg(TcraCl4,TcrbCl4)1Shrm/0	involves: 129/Sv * BALB/c * C57BL/6	MGI:3805339
cx2	Tg(Ins2-HA)165Bri/? Tg(TcraCl4,TcrbCl4)1Shrm/?	involves: BALB/c * C57BL/6 * C57BL/10	MGI:3789209
cx3	Tg(Ins2-HA)165Bri/0 Tg(TcraCl4,TcrbCl4)1Shrm/0	involves: BALB/c * C57BL/6 * C57BL/10 * DBA/2	MGI:3622322
tg4	Tg(TcraCl4,TcrbCl4)1Shrm/?	B10.Cg-H2^d Tg(TcraCl4,TcrbCl4)1Shrm	MGI:3789208

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

weight loss ( J:137838 )

• 4 weeks after receiving transfer of HA-specific effector CD8 T cells, mice exhibit weight loss

behavior/neurological

impaired righting response ( J:137838 )

• 4 weeks after receiving transfer of HA-specific effector CD8 T cells, severely affected animals display difficulty to right themselves when overturned

tremors ( J:137838 )

• 4 weeks after receiving transfer of HA-specific effector CD8 T cells (Tc1 cells), severely affected animals exhibit tremors

decreased locomotor activity ( J:137838 )

• 4 weeks after receiving transfer of HA-specific effector CD8 T cells, severely affected animals show reduced mobility

nervous system

brain inflammation ( J:137838 )

• inflammation is also observed frequently in cerebellum, fornix and periventricular areas

spinal cord inflammation ( J:137838 )

• by 5 days following transfer of Tc1 cells, all mutants examined show spinal cord inflammation

• T cell infiltration into spinal cord starts on day 5 after transfer and peaks at day 28

optic nerve inflammation ( J:137838 )

• by 5 days following transfer of Tc1 cells, all mutants examined show optic nerve inflammation

abnormal oligodendrocyte morphology ( J:137838 )

• apoptosis in optic nerve and spinal cord is seen at early time points following Tc1 transfer

abnormal optic nerve morphology ( J:137838 )

• focal areas with reduced myelin density and thin myelin sheaths are observed in animals after transfer of Tc1 cells

• vacuolization is seen at day 5 after Tc1 cell transfer

demyelination ( J:137838 )

• by 5 days following transfer of Tc1 cells, demyelination is observed in the spinal cord and optic nerve

• from 9 days post-transfer, most mutants exhibit extensive demyelination in optic nerves

• by day 19, actively demyelinating lesions show severe axonal damage

vision/eye

abnormal optic nerve morphology ( J:137838 )

• focal areas with reduced myelin density and thin myelin sheaths are observed in animals after transfer of Tc1 cells

• vacuolization is seen at day 5 after Tc1 cell transfer

immune system

brain inflammation ( J:137838 )

• inflammation is also observed frequently in cerebellum, fornix and periventricular areas

spinal cord inflammation ( J:137838 )

• by 5 days following transfer of Tc1 cells, all mutants examined show spinal cord inflammation

• T cell infiltration into spinal cord starts on day 5 after transfer and peaks at day 28

optic nerve inflammation ( J:137838 )

• by 5 days following transfer of Tc1 cells, all mutants examined show optic nerve inflammation

Allelic Composition	Tg(Ins2-HA)165Bri/? Tg(TcraCl4,TcrbCl4)1Shrm/?
Genetic Background	involves: BALB/c * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ins2-HA)165Bri mutation (3 available)
Tg(TcraCl4,TcrbCl4)1Shrm mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:99756 )

• neonates die within 7 to 10 days of birth due to complications in glucose metabolism

homeostasis/metabolism

hyperglycemia ( J:99756 )

• one day old mice exhibit hyperglycemia with blood glucose levels averaging over 400 mg/dl

• neonates die within 7 to 10 days of birth due to complications in glucose metabolism

endocrine/exocrine glands

insulitis ( J:99756 )

• pancreas of 1-day old mice are infiltrated by transgenic CD8 T cells

• the presence of infiltrating cells disrupts the normal pancreatic architecture with loss of islet mass and dissemination of the remaining islet beta cell clusters

immune system

insulitis ( J:99756 )

• pancreas of 1-day old mice are infiltrated by transgenic CD8 T cells

• the presence of infiltrating cells disrupts the normal pancreatic architecture with loss of islet mass and dissemination of the remaining islet beta cell clusters

Allelic Composition	Tg(Ins2-HA)165Bri/0 Tg(TcraCl4,TcrbCl4)1Shrm/0
Genetic Background	involves: BALB/c * C57BL/6 * C57BL/10 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ins2-HA)165Bri mutation (3 available)
Tg(TcraCl4,TcrbCl4)1Shrm mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality ( J:99756 )

• neonates die within 7-10 days of birth

homeostasis/metabolism

hyperglycemia ( J:99756 )

• neonates exhibit hyperglycemia

endocrine/exocrine glands

abnormal pancreatic islet morphology ( J:99756 )

• animals display a loss of islet mass and dissemination of remaining islet beta-cell clusters

insulitis ( J:99756 )

• In 1-day-old animals, there are many infiltrating cells in the islet and peri-islet regions

immune system

insulitis ( J:99756 )

• In 1-day-old animals, there are many infiltrating cells in the islet and peri-islet regions

Allelic Composition	Tg(TcraCl4,TcrbCl4)1Shrm/?
Genetic Background	B10.Cg-H2^d Tg(TcraCl4,TcrbCl4)1Shrm

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcraCl4,TcrbCl4)1Shrm mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:99756 )

• the majority of mature CD8 T cells in the periphery express the transgenic T cell receptor (TCR) that is specific for influenza virus hemagglutinin (HA) peptide presented by MHC-Class I H2-K^d

• 40% of the CD8 T cells in the blood are reactive to HA peptide presented by H2-K^d

• most thymocytes also express the transgenic TCR

abnormal T cell subpopulation ratio ( J:99756 )

• skewing toward the development of single-positive CD8 T cells is evident in the thymus

• there are 4-fold more CD8 T cells than CD4 T cells found in the lymph nodes

hematopoietic system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:99756 )

• the majority of mature CD8 T cells in the periphery express the transgenic T cell receptor (TCR) that is specific for influenza virus hemagglutinin (HA) peptide presented by MHC-Class I H2-K^d

• 40% of the CD8 T cells in the blood are reactive to HA peptide presented by H2-K^d

• most thymocytes also express the transgenic TCR

abnormal T cell subpopulation ratio ( J:99756 )

• skewing toward the development of single-positive CD8 T cells is evident in the thymus

• there are 4-fold more CD8 T cells than CD4 T cells found in the lymph nodes

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