All Phenotypes Tg(TcraCl4,TcrbCl4)1Shrm MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(HA)Libl/Gt(ROSA)26Sor^tm1(HA)Libl Mog^tm1(cre)Gkl/? Tg(TcraCl4,TcrbCl4)1Shrm/0	involves: 129/Sv * BALB/c * C57BL/6	MGI:3805339
cx2	Tg(Ins2-HA)165Bri/? Tg(TcraCl4,TcrbCl4)1Shrm/?	involves: BALB/c * C57BL/6 * C57BL/10	MGI:3789209
cx3	Tg(Ins2-HA)165Bri/0 Tg(TcraCl4,TcrbCl4)1Shrm/0	involves: BALB/c * C57BL/6 * C57BL/10 * DBA/2	MGI:3622322
tg4	Tg(TcraCl4,TcrbCl4)1Shrm/?	B10.Cg-H2^d Tg(TcraCl4,TcrbCl4)1Shrm	MGI:3789208

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

weight loss ( J:137838 )

• 4 weeks after receiving transfer of HA-specific effector CD8 T cells, mice exhibit weight loss

behavior/neurological

impaired righting response ( J:137838 )

• 4 weeks after receiving transfer of HA-specific effector CD8 T cells, severely affected animals display difficulty to right themselves when overturned

tremors ( J:137838 )

• 4 weeks after receiving transfer of HA-specific effector CD8 T cells (Tc1 cells), severely affected animals exhibit tremors

decreased locomotor activity ( J:137838 )

• 4 weeks after receiving transfer of HA-specific effector CD8 T cells, severely affected animals show reduced mobility

nervous system

brain inflammation ( J:137838 )

• inflammation is also observed frequently in cerebellum, fornix and periventricular areas

spinal cord inflammation ( J:137838 )

• by 5 days following transfer of Tc1 cells, all mutants examined show spinal cord inflammation

• T cell infiltration into spinal cord starts on day 5 after transfer and peaks at day 28

optic nerve inflammation ( J:137838 )

• by 5 days following transfer of Tc1 cells, all mutants examined show optic nerve inflammation

abnormal oligodendrocyte morphology ( J:137838 )

• apoptosis in optic nerve and spinal cord is seen at early time points following Tc1 transfer

abnormal optic nerve morphology ( J:137838 )

• focal areas with reduced myelin density and thin myelin sheaths are observed in animals after transfer of Tc1 cells

• vacuolization is seen at day 5 after Tc1 cell transfer

demyelination ( J:137838 )

• by 5 days following transfer of Tc1 cells, demyelination is observed in the spinal cord and optic nerve

• from 9 days post-transfer, most mutants exhibit extensive demyelination in optic nerves

• by day 19, actively demyelinating lesions show severe axonal damage

vision/eye

abnormal optic nerve morphology ( J:137838 )

• focal areas with reduced myelin density and thin myelin sheaths are observed in animals after transfer of Tc1 cells

• vacuolization is seen at day 5 after Tc1 cell transfer

immune system

brain inflammation ( J:137838 )

• inflammation is also observed frequently in cerebellum, fornix and periventricular areas

spinal cord inflammation ( J:137838 )

• by 5 days following transfer of Tc1 cells, all mutants examined show spinal cord inflammation

• T cell infiltration into spinal cord starts on day 5 after transfer and peaks at day 28

optic nerve inflammation ( J:137838 )

• by 5 days following transfer of Tc1 cells, all mutants examined show optic nerve inflammation

Allelic Composition	Tg(Ins2-HA)165Bri/? Tg(TcraCl4,TcrbCl4)1Shrm/?
Genetic Background	involves: BALB/c * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ins2-HA)165Bri mutation (3 available)
Tg(TcraCl4,TcrbCl4)1Shrm mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:99756 )

• neonates die within 7 to 10 days of birth due to complications in glucose metabolism

homeostasis/metabolism

hyperglycemia ( J:99756 )

• one day old mice exhibit hyperglycemia with blood glucose levels averaging over 400 mg/dl

• neonates die within 7 to 10 days of birth due to complications in glucose metabolism

endocrine/exocrine glands

insulitis ( J:99756 )

• pancreas of 1-day old mice are infiltrated by transgenic CD8 T cells

• the presence of infiltrating cells disrupts the normal pancreatic architecture with loss of islet mass and dissemination of the remaining islet beta cell clusters

immune system

insulitis ( J:99756 )

• pancreas of 1-day old mice are infiltrated by transgenic CD8 T cells

• the presence of infiltrating cells disrupts the normal pancreatic architecture with loss of islet mass and dissemination of the remaining islet beta cell clusters

Allelic Composition	Tg(Ins2-HA)165Bri/0 Tg(TcraCl4,TcrbCl4)1Shrm/0
Genetic Background	involves: BALB/c * C57BL/6 * C57BL/10 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ins2-HA)165Bri mutation (3 available)
Tg(TcraCl4,TcrbCl4)1Shrm mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality ( J:99756 )

• neonates die within 7-10 days of birth

homeostasis/metabolism

hyperglycemia ( J:99756 )

• neonates exhibit hyperglycemia

endocrine/exocrine glands

abnormal pancreatic islet morphology ( J:99756 )

• animals display a loss of islet mass and dissemination of remaining islet beta-cell clusters

insulitis ( J:99756 )

• In 1-day-old animals, there are many infiltrating cells in the islet and peri-islet regions

immune system

insulitis ( J:99756 )

• In 1-day-old animals, there are many infiltrating cells in the islet and peri-islet regions

Allelic Composition	Tg(TcraCl4,TcrbCl4)1Shrm/?
Genetic Background	B10.Cg-H2^d Tg(TcraCl4,TcrbCl4)1Shrm

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcraCl4,TcrbCl4)1Shrm mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:99756 )

• the majority of mature CD8 T cells in the periphery express the transgenic T cell receptor (TCR) that is specific for influenza virus hemagglutinin (HA) peptide presented by MHC-Class I H2-K^d

• 40% of the CD8 T cells in the blood are reactive to HA peptide presented by H2-K^d

• most thymocytes also express the transgenic TCR

abnormal T cell subpopulation ratio ( J:99756 )

• skewing toward the development of single-positive CD8 T cells is evident in the thymus

• there are 4-fold more CD8 T cells than CD4 T cells found in the lymph nodes

hematopoietic system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:99756 )

• the majority of mature CD8 T cells in the periphery express the transgenic T cell receptor (TCR) that is specific for influenza virus hemagglutinin (HA) peptide presented by MHC-Class I H2-K^d

• 40% of the CD8 T cells in the blood are reactive to HA peptide presented by H2-K^d

• most thymocytes also express the transgenic TCR

abnormal T cell subpopulation ratio ( J:99756 )

• skewing toward the development of single-positive CD8 T cells is evident in the thymus

• there are 4-fold more CD8 T cells than CD4 T cells found in the lymph nodes

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 11/12/2024 MGI 6.24