Phenotypes associated with this allele

• Allele Symbol: Onecut2^tm1Flem
• Allele Name: targeted mutation 1, Frederic P Lemaigre
• Allele ID: MGI:3588425
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Onecut2^tm1Flem/Onecut2^tm1Flem	involves: 129S1/Sv * 129X1/SvJ * Swiss	MGI:3608974
cx2	Onecut2^tm1Flem/Onecut2^tm1Flem Onecut3^tm1Jqmn/Onecut3^tm1Jqmn	involves: 129S1/Sv * 129X1/SvJ * Swiss	MGI:3711874
cx3	Onecut1^tm1Flem/Onecut1^tm1Flem Onecut2^tm1Flem/Onecut2^tm1Flem	involves: 129S1/Sv * 129X1/SvJ * Swiss	MGI:3608976

Genotype
MGI:3608974

hm1

• Allelic Composition: Onecut2^tm1Flem/Onecut2^tm1Flem
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * Swiss

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

liver/biliary system

abnormal bile duct development ( J:100352 )

• biliary cells persist in ductal plate configuration or delineate enlarged biliary structures instead of giving rise to the bile ducts

abnormal liver morphology ( J:100352 )

• premature dilations are seen within the ductal plates of embryonic livers

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:121610 )

N • at E10.5, dorsal and ventral pancreatic buds appear normal; similar results show no difference compared to controls at E14.5

N • at E14.5 and E18.5, expression of Neurog3, insulin, and glucagons is comparable to wild-type embryos; endocrine differentiation is normal

N • no enteroendocrine defect is observed; pyloric region is normal, Brunner's glands are present in duodenum, and crypt/villus architecture in small intestine is not altered

N • enteroendocrine cells differentiate normally

abnormal bile duct development ( J:100352 )

• biliary cells persist in ductal plate configuration or delineate enlarged biliary structures instead of giving rise to the bile ducts

Genotype
MGI:3711874

cx2

• Allelic Composition: Onecut2^tm1Flem/Onecut2^tm1Flem; Onecut3^tm1Jqmn/Onecut3^tm1Jqmn
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * Swiss

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:121610 )

N • pancreas organogenesis is normal at all stages in double homozygotes

N • at E14.5 and E18.5, expression of Neurog3, insulin, and glucagons is comparable to wild-type embryos; endocrine differentiation is normal

N • enteroendocrine cells differentiate normally

Genotype
MGI:3608976

cx3

• Allelic Composition: Onecut1^tm1Flem/Onecut1^tm1Flem; Onecut2^tm1Flem/Onecut2^tm1Flem
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * Swiss

liver/biliary system

abnormal hepatoblast differentiation ( J:100352 )

• hepatoblast differentiation is perturbed such that they differentiate into hybrid cells that display characteristics of both hepatocytes and biliary cells

abnormal hepatic cord morphology ( J:100352 )

• cord-like organization of the epithelial cells in the parenchyma is severely perturbed in E15.5 embryos with epithelial cells forming clusters surrounded by hematopoietic cells

endocrine/exocrine glands

abnormal pancreas development ( J:121610 )

• at E13.5, dorsal pancreas is smaller than in Onecut1-deficient embryos; no ventral bud is observed, instead consisting of two small cell clusters on the duodenum

• endocrine differentiation is more affected than in Onecut1-null embryos; pancreas sections almost completely lack Neurog3-expressing cells (~0.5 cells/section)

• insulin- and glucagon-expressing cells are almost absent

• endocrine differentiation initiates normally at E9.5 and progresses comparably to wild-type at E10.5, but becomes defective by E12.5