Phenotypes associated with this allele

• Allele Symbol: Tg(Lck-FADD)1Hed
• Allele Name: transgene insertion 1, Stephen Hedrick
• Allele ID: MGI:3588560
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(Lck-FADD)1Hed/0 Tg(TcraH-Y,TcrbH-Y)71Vbo/0	involves: BALB/c * C57BL/6J * C57L/J * DBA/2J	MGI:3588980
tg2	Tg(Lck-FADD)1Hed/Tg(Lck-FADD)1Hed	involves: BALB/c * C57BL/6	MGI:3588669
tg3	Tg(Lck-FADD)1Hed/0	involves: BALB/c * C57BL/6	MGI:3588668
tg4	Tg(Lck-FADD)1Hed/?	involves: BALB/c * C57BL/6	MGI:3588670

Genotype
MGI:3588980

cx1

• Allelic Composition: Tg(Lck-FADD)1Hed/0; Tg(TcraH-Y,TcrbH-Y)71Vbo/0
• Genetic Background: involves: BALB/c * C57BL/6J * C57L/J * DBA/2J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:100451 )

N • HY mediated negative selection in this TCR transgenic model was not blocked or altered by the presence of Tg(Lck-FADD)1Hed

Genotype
MGI:3588669

tg2

• Allelic Composition: Tg(Lck-FADD)1Hed/Tg(Lck-FADD)1Hed
• Genetic Background: involves: BALB/c * C57BL/6

immune system

immune system phenotype ( J:100451 )

N • normal levels of TCR surface expression

abnormal T cell differentiation ( J:100451 )

• partial block of thymocyte development at the CD25+CD44- stage of development just prior to proliferation

decreased T cell number ( J:100451 )

• T cell levels in spleen only 3% of normal levels at 3 to 4 weeks of age

decreased thymocyte number ( J:100451 )

• total number of thymocytes is approximately 35% that of wildtype littermates

hematopoietic system

abnormal T cell differentiation ( J:100451 )

• partial block of thymocyte development at the CD25+CD44- stage of development just prior to proliferation

decreased T cell number ( J:100451 )

• T cell levels in spleen only 3% of normal levels at 3 to 4 weeks of age

decreased thymocyte number ( J:100451 )

• total number of thymocytes is approximately 35% that of wildtype littermates

endocrine/exocrine glands

decreased thymocyte number ( J:100451 )

• total number of thymocytes is approximately 35% that of wildtype littermates

Genotype
MGI:3588668

tg3

• Allelic Composition: Tg(Lck-FADD)1Hed/0
• Genetic Background: involves: BALB/c * C57BL/6

hematopoietic system

abnormal T cell proliferation ( J:100451 )

• lymph node T cells respond almost normally to plate-bound anti-CD3 and anti-CD28 but are deficient in response to anti-CD3 and PMA or anti-CD3 and calcium ionophore

• unlike wildtype lymph node T cells or heterozygous thymocytes heterozygous lymph node T cells proliferate in response to PMA alone

arrested T cell differentiation ( J:100451 )

• partial block of thymocytes development at the CD25+CD44- stage of development just prior to proliferation

decreased T cell number ( J:100451 )

• T cell levels in spleen only 20% of normal levels at 3 to 4 weeks of age

• CD4+ spleen and lymph node T cells increase with age to wild-type levels but CD8+ remain at low levels

decreased thymocyte number ( J:100451 )

• decrease in total thymocyte number

immune system

abnormal T cell proliferation ( J:100451 )

• lymph node T cells respond almost normally to plate-bound anti-CD3 and anti-CD28 but are deficient in response to anti-CD3 and PMA or anti-CD3 and calcium ionophore

• unlike wildtype lymph node T cells or heterozygous thymocytes heterozygous lymph node T cells proliferate in response to PMA alone

arrested T cell differentiation ( J:100451 )

• partial block of thymocytes development at the CD25+CD44- stage of development just prior to proliferation

decreased T cell number ( J:100451 )

• T cell levels in spleen only 20% of normal levels at 3 to 4 weeks of age

• CD4+ spleen and lymph node T cells increase with age to wild-type levels but CD8+ remain at low levels

decreased thymocyte number ( J:100451 )

• decrease in total thymocyte number

endocrine/exocrine glands

decreased thymocyte number ( J:100451 )

• decrease in total thymocyte number

cellular

abnormal T cell proliferation ( J:100451 )

• lymph node T cells respond almost normally to plate-bound anti-CD3 and anti-CD28 but are deficient in response to anti-CD3 and PMA or anti-CD3 and calcium ionophore

• unlike wildtype lymph node T cells or heterozygous thymocytes heterozygous lymph node T cells proliferate in response to PMA alone

Genotype
MGI:3588670

tg4

• Allelic Composition: Tg(Lck-FADD)1Hed/?
• Genetic Background: involves: BALB/c * C57BL/6

immune system

immune system phenotype ( J:100451 )

N • no indication of lymphoproliferative disease or development of CD3+B220+ population was observed out to 1.5 years of age

abnormal T cell physiology ( J:100451 )

• anti-CD95 plus cycloheximide fail to induce change in thymic mitochondrial potential

decreased thymocyte apoptosis ( J:100451 )

• CD95 induced apoptosis in thymocytes is blocked but thapsigargan, dexamethasone, and staurosporine each still induce thymocyte apoptosis

hematopoietic system

abnormal T cell physiology ( J:100451 )

• anti-CD95 plus cycloheximide fail to induce change in thymic mitochondrial potential

decreased thymocyte apoptosis ( J:100451 )

• CD95 induced apoptosis in thymocytes is blocked but thapsigargan, dexamethasone, and staurosporine each still induce thymocyte apoptosis

cellular

decreased thymocyte apoptosis ( J:100451 )

• CD95 induced apoptosis in thymocytes is blocked but thapsigargan, dexamethasone, and staurosporine each still induce thymocyte apoptosis

endocrine/exocrine glands

decreased thymocyte apoptosis ( J:100451 )

• CD95 induced apoptosis in thymocytes is blocked but thapsigargan, dexamethasone, and staurosporine each still induce thymocyte apoptosis